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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Nature communications
2024
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39496591/ |
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| author | Nou, Nancy O Covington, Jonathan K Lai, Dengxun Mayali, Xavier Seymour, Cale O Johnston, Juliet Jiao, Jian-Yu Buessecker, Steffen Mosier, Damon Muok, Alise R Torosian, Nicole Cook, Allison M Briegel, Ariane Woyke, Tanja Eloe-Fadrosh, Emiley Shapiro, Nicole Bryan, Scott G Sleezer, Savannah Dimapilis, Joshua Gonzalez, Cristina Gonzalez, Lizett Noriega, Marlene Hess, Matthias Carlson, Ross P Liu, Lan Li, Meng-Meng Lian, Zheng-Han Zhu, Siqi Liu, Fan Sun, Xian Gao, Beile Mewalal, Ritesh Harmon-Smith, Miranda Blaby, Ian K Cheng, Jan-Fang Weber, Peter K Grigorean, Gabriela Li, Wen-Jun Dekas, Anne E Pett-Ridge, Jennifer Dodsworth, Jeremy A Palmer, Marike Hedlund, Brian P |
| author_facet | Nou, Nancy O Covington, Jonathan K Lai, Dengxun Mayali, Xavier Seymour, Cale O Johnston, Juliet Jiao, Jian-Yu Buessecker, Steffen Mosier, Damon Muok, Alise R Torosian, Nicole Cook, Allison M Briegel, Ariane Woyke, Tanja Eloe-Fadrosh, Emiley Shapiro, Nicole Bryan, Scott G Sleezer, Savannah Dimapilis, Joshua Gonzalez, Cristina Gonzalez, Lizett Noriega, Marlene Hess, Matthias Carlson, Ross P Liu, Lan Li, Meng-Meng Lian, Zheng-Han Zhu, Siqi Liu, Fan Sun, Xian Gao, Beile Mewalal, Ritesh Harmon-Smith, Miranda Blaby, Ian K Cheng, Jan-Fang Weber, Peter K Grigorean, Gabriela Li, Wen-Jun Dekas, Anne E Pett-Ridge, Jennifer Dodsworth, Jeremy A Palmer, Marike Hedlund, Brian P Nou, Nancy O Covington, Jonathan K Lai, Dengxun Mayali, Xavier Seymour, Cale O Johnston, Juliet Jiao, Jian-Yu Buessecker, Steffen Mosier, Damon Muok, Alise R Torosian, Nicole Cook, Allison M Briegel, Ariane Woyke, Tanja Eloe-Fadrosh, Emiley Shapiro, Nicole Bryan, Scott G Sleezer, Savannah Dimapilis, Joshua Gonzalez, Cristina Gonzalez, Lizett Noriega, Marlene Hess, Matthias Carlson, Ross P Liu, Lan Li, Meng-Meng Lian, Zheng-Han Zhu, Siqi Liu, Fan Sun, Xian Gao, Beile Mewalal, Ritesh Harmon-Smith, Miranda Blaby, Ian K Cheng, Jan-Fang Weber, Peter K Grigorean, Gabriela Li, Wen-Jun Dekas, Anne E Pett-Ridge, Jennifer Dodsworth, Jeremy A Palmer, Marike Hedlund, Brian P |
| collection | PubMed - marine biology |
| contents | Genome-guided isolation of the hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota. Nou, Nancy O Covington, Jonathan K Lai, Dengxun Mayali, Xavier Seymour, Cale O Johnston, Juliet Jiao, Jian-Yu Buessecker, Steffen Mosier, Damon Muok, Alise R Torosian, Nicole Cook, Allison M Briegel, Ariane Woyke, Tanja Eloe-Fadrosh, Emiley Shapiro, Nicole Bryan, Scott G Sleezer, Savannah Dimapilis, Joshua Gonzalez, Cristina Gonzalez, Lizett Noriega, Marlene Hess, Matthias Carlson, Ross P Liu, Lan Li, Meng-Meng Lian, Zheng-Han Zhu, Siqi Liu, Fan Sun, Xian Gao, Beile Mewalal, Ritesh Harmon-Smith, Miranda Blaby, Ian K Cheng, Jan-Fang Weber, Peter K Grigorean, Gabriela Li, Wen-Jun Dekas, Anne E Pett-Ridge, Jennifer Dodsworth, Jeremy A Palmer, Marike Hedlund, Brian P Polysaccharides Genome, Bacterial Glycoside Hydrolases Phylogeny Bacterial Proteins Carbohydrate Metabolism Aerobiosis Hot Springs Bacteria Proteomics Few aerobic hyperthermophilic microorganisms degrade polysaccharides. Here, we describe the genome-enabled enrichment and optical tweezer-based isolation of an aerobic polysaccharide-degrading hyperthermophile, Fervidibacter sacchari, previously ascribed to candidate phylum Fervidibacteria. F. sacchari uses polysaccharides and monosaccharides for growth at 65-87.5 °C and expresses 191 carbohydrate-active enzymes (CAZymes) according to RNA-Seq and proteomics, including 31 with unusual glycoside hydrolase domains (GH109, GH177, GH179). Fluorescence in-situ hybridization and nanoscale secondary ion mass spectrometry confirmed rapid assimilation of C-starch in spring sediments. Purified GHs were optimally active at 80-100 °C on ten different polysaccharides. Finally, we propose reassigning Fervidibacteria as a class within phylum Armatimonadota, along with 18 other species, and show that a high number and diversity of CAZymes is a hallmark of the phylum, in both aerobic and anaerobic lineages. Our study establishes Fervidibacteria as hyperthermophilic polysaccharide degraders in terrestrial geothermal springs and suggests a broad role for Armatimonadota in polysaccharide catabolism. |
| format | Artículo científico |
| id | pubmed_39496591 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Nature communications |
| record_format | pubmed |
| spellingShingle | Genome-guided isolation of the hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota. Nou, Nancy O Covington, Jonathan K Lai, Dengxun Mayali, Xavier Seymour, Cale O Johnston, Juliet Jiao, Jian-Yu Buessecker, Steffen Mosier, Damon Muok, Alise R Torosian, Nicole Cook, Allison M Briegel, Ariane Woyke, Tanja Eloe-Fadrosh, Emiley Shapiro, Nicole Bryan, Scott G Sleezer, Savannah Dimapilis, Joshua Gonzalez, Cristina Gonzalez, Lizett Noriega, Marlene Hess, Matthias Carlson, Ross P Liu, Lan Li, Meng-Meng Lian, Zheng-Han Zhu, Siqi Liu, Fan Sun, Xian Gao, Beile Mewalal, Ritesh Harmon-Smith, Miranda Blaby, Ian K Cheng, Jan-Fang Weber, Peter K Grigorean, Gabriela Li, Wen-Jun Dekas, Anne E Pett-Ridge, Jennifer Dodsworth, Jeremy A Palmer, Marike Hedlund, Brian P Polysaccharides Genome, Bacterial Glycoside Hydrolases Phylogeny Bacterial Proteins Carbohydrate Metabolism Aerobiosis Hot Springs Bacteria Proteomics Genome-guided isolation of the hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota. Nou, Nancy O Covington, Jonathan K Lai, Dengxun Mayali, Xavier Seymour, Cale O Johnston, Juliet Jiao, Jian-Yu Buessecker, Steffen Mosier, Damon Muok, Alise R Torosian, Nicole Cook, Allison M Briegel, Ariane Woyke, Tanja Eloe-Fadrosh, Emiley Shapiro, Nicole Bryan, Scott G Sleezer, Savannah Dimapilis, Joshua Gonzalez, Cristina Gonzalez, Lizett Noriega, Marlene Hess, Matthias Carlson, Ross P Liu, Lan Li, Meng-Meng Lian, Zheng-Han Zhu, Siqi Liu, Fan Sun, Xian Gao, Beile Mewalal, Ritesh Harmon-Smith, Miranda Blaby, Ian K Cheng, Jan-Fang Weber, Peter K Grigorean, Gabriela Li, Wen-Jun Dekas, Anne E Pett-Ridge, Jennifer Dodsworth, Jeremy A Palmer, Marike Hedlund, Brian P Polysaccharides Genome, Bacterial Glycoside Hydrolases Phylogeny Bacterial Proteins Carbohydrate Metabolism Aerobiosis Hot Springs Bacteria Proteomics Few aerobic hyperthermophilic microorganisms degrade polysaccharides. Here, we describe the genome-enabled enrichment and optical tweezer-based isolation of an aerobic polysaccharide-degrading hyperthermophile, Fervidibacter sacchari, previously ascribed to candidate phylum Fervidibacteria. F. sacchari uses polysaccharides and monosaccharides for growth at 65-87.5 °C and expresses 191 carbohydrate-active enzymes (CAZymes) according to RNA-Seq and proteomics, including 31 with unusual glycoside hydrolase domains (GH109, GH177, GH179). Fluorescence in-situ hybridization and nanoscale secondary ion mass spectrometry confirmed rapid assimilation of C-starch in spring sediments. Purified GHs were optimally active at 80-100 °C on ten different polysaccharides. Finally, we propose reassigning Fervidibacteria as a class within phylum Armatimonadota, along with 18 other species, and show that a high number and diversity of CAZymes is a hallmark of the phylum, in both aerobic and anaerobic lineages. Our study establishes Fervidibacteria as hyperthermophilic polysaccharide degraders in terrestrial geothermal springs and suggests a broad role for Armatimonadota in polysaccharide catabolism. |
| title | Genome-guided isolation of the hyperthermophilic aerobe Fervidibacter sacchari reveals conserved polysaccharide metabolism in the Armatimonadota. |
| topic | Polysaccharides Genome, Bacterial Glycoside Hydrolases Phylogeny Bacterial Proteins Carbohydrate Metabolism Aerobiosis Hot Springs Bacteria Proteomics |
| url | https://pubmed.ncbi.nlm.nih.gov/39496591/ |