Oligonol, an Oligomerized Polyphenol from , Enhances Branched-Chain Amino Acid Transportation and Catabolism to Alleviate Sarcopenia.
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| Autori principali: | , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
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International journal of molecular sciences
2024
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| _version_ | 1868266282462216192 |
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| author | Chang, Yun-Ching Chen, Yu-Chi Chan, Yin-Ching Liu, Cheng Chang, Sue-Joan |
| author_facet | Chang, Yun-Ching Chen, Yu-Chi Chan, Yin-Ching Liu, Cheng Chang, Sue-Joan Chang, Yun-Ching Chen, Yu-Chi Chan, Yin-Ching Liu, Cheng Chang, Sue-Joan |
| collection | PubMed - marine biology |
| contents | Oligonol, an Oligomerized Polyphenol from , Enhances Branched-Chain Amino Acid Transportation and Catabolism to Alleviate Sarcopenia. Chang, Yun-Ching Chen, Yu-Chi Chan, Yin-Ching Liu, Cheng Chang, Sue-Joan Animals Litchi Amino Acids, Branched-Chain Sarcopenia Mice Muscle, Skeletal Mice, Inbred C57BL Transaminases Male Polyphenols Catechin TOR Serine-Threonine Kinases Cell Line Phenols Branched-chain amino acids (BCAAs) are essential for muscle protein synthesis and are widely acknowledged for mitigating sarcopenia. Oligonol (Olg), a low-molecular-weight polyphenol from , has also been found to attenuate sarcopenia by improving mitochondrial quality and positive protein turnover. This study aims to investigate the effect of Olg on BCAA-stimulated protein synthesis in sarcopenia. In sarcopenic C57BL/6 mice and senescence-accelerated mouse-prone 8 (SAMP8) mice, BCAAs were significantly decreased in skeletal muscle but increased in blood serum. Furthermore, the expressions of membrane L-type amino acid transporter 1 (LAT1) and branched-chain amino acid transaminase 2 (BCAT2) in skeletal muscle were lower in aged mice than in young mice. The administration of Olg for 8 weeks significantly increased the expressions of membrane LAT1 and BCAT2 in the skeletal muscle when compared with non-treated SAMP8 mice. We further found that BCAA deprivation via LAT1-siRNA in C2C12 myotubes inhibited the signaling of protein synthesis and facilitated ubiquitination degradation of BCAT2. In C2C12 cells mimicking sarcopenia, Olg combined with BCAA supplementation enhanced mTOR/p70S6K activity more than BCAA alone. However, blocked LAT1 by JPH203 reversed the synergistic effect of the combination of Olg and BCAAs. Taken together, changes in LAT1 and BCAT2 during aging profoundly alter BCAA availability and nutrient signaling in aged mice. Olg increases BCAA-stimulated protein synthesis via modulating BCAA transportation and BCAA catabolism. Combining Olg and BCAAs may be a useful nutritional strategy for alleviating sarcopenia. |
| format | Artículo científico |
| id | pubmed_39519101 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | International journal of molecular sciences |
| record_format | pubmed |
| spellingShingle | Oligonol, an Oligomerized Polyphenol from , Enhances Branched-Chain Amino Acid Transportation and Catabolism to Alleviate Sarcopenia. Chang, Yun-Ching Chen, Yu-Chi Chan, Yin-Ching Liu, Cheng Chang, Sue-Joan Animals Litchi Amino Acids, Branched-Chain Sarcopenia Mice Muscle, Skeletal Mice, Inbred C57BL Transaminases Male Polyphenols Catechin TOR Serine-Threonine Kinases Cell Line Phenols Oligonol, an Oligomerized Polyphenol from , Enhances Branched-Chain Amino Acid Transportation and Catabolism to Alleviate Sarcopenia. Chang, Yun-Ching Chen, Yu-Chi Chan, Yin-Ching Liu, Cheng Chang, Sue-Joan Animals Litchi Amino Acids, Branched-Chain Sarcopenia Mice Muscle, Skeletal Mice, Inbred C57BL Transaminases Male Polyphenols Catechin TOR Serine-Threonine Kinases Cell Line Phenols Branched-chain amino acids (BCAAs) are essential for muscle protein synthesis and are widely acknowledged for mitigating sarcopenia. Oligonol (Olg), a low-molecular-weight polyphenol from , has also been found to attenuate sarcopenia by improving mitochondrial quality and positive protein turnover. This study aims to investigate the effect of Olg on BCAA-stimulated protein synthesis in sarcopenia. In sarcopenic C57BL/6 mice and senescence-accelerated mouse-prone 8 (SAMP8) mice, BCAAs were significantly decreased in skeletal muscle but increased in blood serum. Furthermore, the expressions of membrane L-type amino acid transporter 1 (LAT1) and branched-chain amino acid transaminase 2 (BCAT2) in skeletal muscle were lower in aged mice than in young mice. The administration of Olg for 8 weeks significantly increased the expressions of membrane LAT1 and BCAT2 in the skeletal muscle when compared with non-treated SAMP8 mice. We further found that BCAA deprivation via LAT1-siRNA in C2C12 myotubes inhibited the signaling of protein synthesis and facilitated ubiquitination degradation of BCAT2. In C2C12 cells mimicking sarcopenia, Olg combined with BCAA supplementation enhanced mTOR/p70S6K activity more than BCAA alone. However, blocked LAT1 by JPH203 reversed the synergistic effect of the combination of Olg and BCAAs. Taken together, changes in LAT1 and BCAT2 during aging profoundly alter BCAA availability and nutrient signaling in aged mice. Olg increases BCAA-stimulated protein synthesis via modulating BCAA transportation and BCAA catabolism. Combining Olg and BCAAs may be a useful nutritional strategy for alleviating sarcopenia. |
| title | Oligonol, an Oligomerized Polyphenol from , Enhances Branched-Chain Amino Acid Transportation and Catabolism to Alleviate Sarcopenia. |
| topic | Animals Litchi Amino Acids, Branched-Chain Sarcopenia Mice Muscle, Skeletal Mice, Inbred C57BL Transaminases Male Polyphenols Catechin TOR Serine-Threonine Kinases Cell Line Phenols |
| url | https://pubmed.ncbi.nlm.nih.gov/39519101/ |