Overexpression of PtNRPS1 enhances diatom-mediated bioremediation of salicylate pollution.
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| Format: | Artículo científico |
| Langue: | en |
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Bioresource technology
2025
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| _version_ | 1868266282456973314 |
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| author | Zhao, Dong-Sheng Chen, Yu-Ting Xu, Jia-Min Liu, Xiao-Li Xu, Yi-Cheng Cao, Peng Li, Junliang Wang, Shengqin Li, Nan Li, Yong Li, Shu-Ming Yan, Xiufeng Pang, Qiuying Zou, Hui-Xi |
| author_facet | Zhao, Dong-Sheng Chen, Yu-Ting Xu, Jia-Min Liu, Xiao-Li Xu, Yi-Cheng Cao, Peng Li, Junliang Wang, Shengqin Li, Nan Li, Yong Li, Shu-Ming Yan, Xiufeng Pang, Qiuying Zou, Hui-Xi Zhao, Dong-Sheng Chen, Yu-Ting Xu, Jia-Min Liu, Xiao-Li Xu, Yi-Cheng Cao, Peng Li, Junliang Wang, Shengqin Li, Nan Li, Yong Li, Shu-Ming Yan, Xiufeng Pang, Qiuying Zou, Hui-Xi |
| collection | PubMed - marine biology |
| contents | Overexpression of PtNRPS1 enhances diatom-mediated bioremediation of salicylate pollution. Zhao, Dong-Sheng Chen, Yu-Ting Xu, Jia-Min Liu, Xiao-Li Xu, Yi-Cheng Cao, Peng Li, Junliang Wang, Shengqin Li, Nan Li, Yong Li, Shu-Ming Yan, Xiufeng Pang, Qiuying Zou, Hui-Xi Diatoms Biodegradation, Environmental Salicylic Acid Water Pollutants, Chemical Peptide Synthases Salicylates The accumulation of the emerging pollutant salicylic acid (SA) in the environment has gained much attention. In this study, overexpression of the non-ribosomal peptide synthase (NRPS) gene, PtNRPS1 in the marine diatom Phaeodactylum tricornutum (PtNRPS1-OE) increased resistance to SA pollutants. It was assumed that the enhanced tolerance was due to the high binding affinity between recombinant PtNRPS1 (rNRPS1) and SA pollutants. Moreover, tandem mass spectrometry analysis determined the amino acids that participated in the covalently binding of SA. The removal efficiency of SA pollutants by PtNRPS1-OE cells was found to be markedly elevated. The mechanism underlying the removal of SA and 5-substituted SA (5-sSA) was proposed, following the co-localization analysis of rNRPS1 and SA. The purpose of this study was not about using PtNRPS1 as an enzyme to catalyze the synthesis of metabolite. Rather, it explored the possibility of using PtNRPS1 to remove pollutants, which further improves practical feasibility of microalgae-mediated bioremediation. |
| format | Artículo científico |
| id | pubmed_39521186 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Bioresource technology |
| record_format | pubmed |
| spellingShingle | Overexpression of PtNRPS1 enhances diatom-mediated bioremediation of salicylate pollution. Zhao, Dong-Sheng Chen, Yu-Ting Xu, Jia-Min Liu, Xiao-Li Xu, Yi-Cheng Cao, Peng Li, Junliang Wang, Shengqin Li, Nan Li, Yong Li, Shu-Ming Yan, Xiufeng Pang, Qiuying Zou, Hui-Xi Diatoms Biodegradation, Environmental Salicylic Acid Water Pollutants, Chemical Peptide Synthases Salicylates Overexpression of PtNRPS1 enhances diatom-mediated bioremediation of salicylate pollution. Zhao, Dong-Sheng Chen, Yu-Ting Xu, Jia-Min Liu, Xiao-Li Xu, Yi-Cheng Cao, Peng Li, Junliang Wang, Shengqin Li, Nan Li, Yong Li, Shu-Ming Yan, Xiufeng Pang, Qiuying Zou, Hui-Xi Diatoms Biodegradation, Environmental Salicylic Acid Water Pollutants, Chemical Peptide Synthases Salicylates The accumulation of the emerging pollutant salicylic acid (SA) in the environment has gained much attention. In this study, overexpression of the non-ribosomal peptide synthase (NRPS) gene, PtNRPS1 in the marine diatom Phaeodactylum tricornutum (PtNRPS1-OE) increased resistance to SA pollutants. It was assumed that the enhanced tolerance was due to the high binding affinity between recombinant PtNRPS1 (rNRPS1) and SA pollutants. Moreover, tandem mass spectrometry analysis determined the amino acids that participated in the covalently binding of SA. The removal efficiency of SA pollutants by PtNRPS1-OE cells was found to be markedly elevated. The mechanism underlying the removal of SA and 5-substituted SA (5-sSA) was proposed, following the co-localization analysis of rNRPS1 and SA. The purpose of this study was not about using PtNRPS1 as an enzyme to catalyze the synthesis of metabolite. Rather, it explored the possibility of using PtNRPS1 to remove pollutants, which further improves practical feasibility of microalgae-mediated bioremediation. |
| title | Overexpression of PtNRPS1 enhances diatom-mediated bioremediation of salicylate pollution. |
| topic | Diatoms Biodegradation, Environmental Salicylic Acid Water Pollutants, Chemical Peptide Synthases Salicylates |
| url | https://pubmed.ncbi.nlm.nih.gov/39521186/ |