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| Auteurs principaux: | , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Analytical chemistry
2024
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| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/39531253/ |
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Table des matières:
- Ultrafast Evolution of Bacterial Antimicrobial Resistance by Picoliter-Scale Centrifugal Microfluidics. Xu, Teng Dai, Yajie Ge, Anle Chen, Xueqian Gong, Yanhai Lam, Tze Hau Lee, Kelvin Han, Xiao Ji, Yuetong Shen, Wei Liu, Jiquan Sun, Luyang Xu, Jian Ma, Bo Escherichia coli Microbial Sensitivity Tests Anti-Bacterial Agents Drug Resistance, Bacterial Ciprofloxacin Centrifugation Amikacin Triclosan Microfluidic Analytical Techniques Microfluidics Experimental evolution is a powerful approach for scrutinizing and dissecting the development of antimicrobial resistance; nevertheless, it typically demands an extended duration to detect evolutionary changes. Here, a centrifugal microfluidics system is designed to accelerate the process. Through a simple step of on-chip centrifugation, a highly condensed bacterial matrix of ∼10 cells/mL at the enrichment tip of the chip channel is derived, enabling bacteria encapsulated to survive in antimicrobial concentrations several times higher than the minimum inhibitory concentration (MIC) and rapidly develop resistance in the first 10 h. After 48 h of on-chip evolution, the strain demonstrated a 64 to 128-fold reduction in sensitivity to disinfectants (triclosan) as well as antibiotics (ciprofloxacin and amikacin), a rate substantially swifter compared to conventional continuous inoculation-based experimental evolution. The speed and simplicity of this microfluidic system suggest its broad application for uncovering resistance mechanisms and identifying targets of biocides and antibiotics.