Exosome-mediated viral nucleic acid presentation in a crustacean expounds innate immunity from a novel perspective.
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| Autori principali: | , , , , , , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Journal of virology
2024
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| _version_ | 1868266280136474626 |
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| author | Gong, Yi Hu, Hang Zhao, Xinshan Wei, Weiqian Zhang, Ming Tran, Ngoc Tuan Ma, Hongyu Zhang, Yueling Chan, Kok-Gan Li, Shengkang |
| author_facet | Gong, Yi Hu, Hang Zhao, Xinshan Wei, Weiqian Zhang, Ming Tran, Ngoc Tuan Ma, Hongyu Zhang, Yueling Chan, Kok-Gan Li, Shengkang Gong, Yi Hu, Hang Zhao, Xinshan Wei, Weiqian Zhang, Ming Tran, Ngoc Tuan Ma, Hongyu Zhang, Yueling Chan, Kok-Gan Li, Shengkang |
| collection | PubMed - marine biology |
| contents | Exosome-mediated viral nucleic acid presentation in a crustacean expounds innate immunity from a novel perspective. Gong, Yi Hu, Hang Zhao, Xinshan Wei, Weiqian Zhang, Ming Tran, Ngoc Tuan Ma, Hongyu Zhang, Yueling Chan, Kok-Gan Li, Shengkang Animals Exosomes Immunity, Innate White spot syndrome virus 1 Hemocytes Brachyura Apoptosis Host-Pathogen Interactions DNA, Viral As an enduring hot topic in the field of innate immunity, apoptosis is widely considered an effective approach to eliminate pathogenic microbes and plays a crucial role during host-pathogen interactions. Recently, researchers have found that the virus-containing host cells could transmit apoptotic signals to the surrounding uninfected cells during infection, but the mechanism remains unclear. Here, we found that exosomes secreted by WSSV-infected mud crab hemocytes contain viral nucleic acid wsv277, which could be transported to the recipient cells and further expressed viral protein with phosphokinase activity. Besides, by using transcriptome, proteome, ChIP-seq, and coIP techniques, the results revealed that wsv277 could activate the transcription and translation of apoptotic genes via interacting with CBF and EF-1α so as to suppress the spread of virus infection by inducing apoptosis of the surrounding cells. Therefore, for the first time, our study proved that the components of DNA virus could be encapsulated into exosomes and elucidated the mechanism of apoptotic signal transduction between cells from the perspective of exosomes. Our study revealed that the components of DNA virus could be packaged and transmitted through the exosomes of lower invertebrates, which strongly demonstrated the diversity of exosome-mediated viral immunity and its universality in animals. Furthermore, we elucidated the mechanism of apoptotic signal transduction between cells from the perspective of exosomes and revealed a novel strategy for the host to cope with viral infection. |
| format | Artículo científico |
| id | pubmed_39545727 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Journal of virology |
| record_format | pubmed |
| spellingShingle | Exosome-mediated viral nucleic acid presentation in a crustacean expounds innate immunity from a novel perspective. Gong, Yi Hu, Hang Zhao, Xinshan Wei, Weiqian Zhang, Ming Tran, Ngoc Tuan Ma, Hongyu Zhang, Yueling Chan, Kok-Gan Li, Shengkang Animals Exosomes Immunity, Innate White spot syndrome virus 1 Hemocytes Brachyura Apoptosis Host-Pathogen Interactions DNA, Viral Exosome-mediated viral nucleic acid presentation in a crustacean expounds innate immunity from a novel perspective. Gong, Yi Hu, Hang Zhao, Xinshan Wei, Weiqian Zhang, Ming Tran, Ngoc Tuan Ma, Hongyu Zhang, Yueling Chan, Kok-Gan Li, Shengkang Animals Exosomes Immunity, Innate White spot syndrome virus 1 Hemocytes Brachyura Apoptosis Host-Pathogen Interactions DNA, Viral As an enduring hot topic in the field of innate immunity, apoptosis is widely considered an effective approach to eliminate pathogenic microbes and plays a crucial role during host-pathogen interactions. Recently, researchers have found that the virus-containing host cells could transmit apoptotic signals to the surrounding uninfected cells during infection, but the mechanism remains unclear. Here, we found that exosomes secreted by WSSV-infected mud crab hemocytes contain viral nucleic acid wsv277, which could be transported to the recipient cells and further expressed viral protein with phosphokinase activity. Besides, by using transcriptome, proteome, ChIP-seq, and coIP techniques, the results revealed that wsv277 could activate the transcription and translation of apoptotic genes via interacting with CBF and EF-1α so as to suppress the spread of virus infection by inducing apoptosis of the surrounding cells. Therefore, for the first time, our study proved that the components of DNA virus could be encapsulated into exosomes and elucidated the mechanism of apoptotic signal transduction between cells from the perspective of exosomes. Our study revealed that the components of DNA virus could be packaged and transmitted through the exosomes of lower invertebrates, which strongly demonstrated the diversity of exosome-mediated viral immunity and its universality in animals. Furthermore, we elucidated the mechanism of apoptotic signal transduction between cells from the perspective of exosomes and revealed a novel strategy for the host to cope with viral infection. |
| title | Exosome-mediated viral nucleic acid presentation in a crustacean expounds innate immunity from a novel perspective. |
| topic | Animals Exosomes Immunity, Innate White spot syndrome virus 1 Hemocytes Brachyura Apoptosis Host-Pathogen Interactions DNA, Viral |
| url | https://pubmed.ncbi.nlm.nih.gov/39545727/ |