sp. nov. and gen. nov., sp. nov., isolated from freshwater environments in China and re-examining the taxonomic status of genera and .
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| Format: | Artículo científico |
| Sprache: | en |
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International journal of systematic and evolutionary microbiology
2024
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| _version_ | 1868266280120745984 |
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| author | Lu, Huibin Chen, Li Wang, Yujing Xing, Peng Wu, Qinglong |
| author_facet | Lu, Huibin Chen, Li Wang, Yujing Xing, Peng Wu, Qinglong Lu, Huibin Chen, Li Wang, Yujing Xing, Peng Wu, Qinglong |
| collection | PubMed - marine biology |
| contents | sp. nov. and gen. nov., sp. nov., isolated from freshwater environments in China and re-examining the taxonomic status of genera and . Lu, Huibin Chen, Li Wang, Yujing Xing, Peng Wu, Qinglong Phylogeny China RNA, Ribosomal, 16S Fresh Water DNA, Bacterial Bacterial Typing Techniques Sequence Analysis, DNA Nucleic Acid Hybridization Lysobacter Fatty Acids Base Composition Xanthomonadaceae Three Gram-stain-negative, aerobic, short rod-shaped and motile strains (FXH3W, SHGZ20W and SMYT11W) were isolated from freshwater environments in China. Comparisons based on the 16S rRNA gene sequences indicated that strains FXH3W and SHGZ20W showed the highest 16S rRNA gene sequence similarity of about 99.6% to '' MIC 1.5, and strain SMYT11W showed the highest 16S rRNA gene sequence similarity of 99.8% to D4P002, respectively. Observing the phylogenetic trees reconstructed based on the 16S rRNA gene sequences, the species of genera and were not monophyletic and often mixed together. The further reconstructed phylogenomic tree and Genome Taxonomy Database also showed that the species of both genera were polyphyletic, implying that the current taxonomic status of the species of both genera was questionable. The calculated OrthoANIu, digital DNA-DNA hybridization and average amino acid sequence identity (AAI) values supported that strains FXH3W and SHGZ20W should belong to the same novel species and strain SMYT11W should also represent an independent novel species. Combining the AAI values and phylogenomic analyses, the species of genera and should be reassigned to 12 genera (, , , , , , , , , , and ). The AAI values 69.5-76.0% were also proposed as the -specific thresholds for genus delineation. Strain SMYT11W should represent a novel species of the genus , for which the name sp. nov. (type strains SMYT11W=GDMCC 1.4275=KCTC 8304) is proposed. Strains FXH3W and SHGZ20W should represent a novel species of a new genus gen. nov., sp. nov. The type strain of the type species is FXH3W (=GDMCC 1.4096=KCTC 8154). |
| format | Artículo científico |
| id | pubmed_39556494 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | International journal of systematic and evolutionary microbiology |
| record_format | pubmed |
| spellingShingle | sp. nov. and gen. nov., sp. nov., isolated from freshwater environments in China and re-examining the taxonomic status of genera and . Lu, Huibin Chen, Li Wang, Yujing Xing, Peng Wu, Qinglong Phylogeny China RNA, Ribosomal, 16S Fresh Water DNA, Bacterial Bacterial Typing Techniques Sequence Analysis, DNA Nucleic Acid Hybridization Lysobacter Fatty Acids Base Composition Xanthomonadaceae sp. nov. and gen. nov., sp. nov., isolated from freshwater environments in China and re-examining the taxonomic status of genera and . Lu, Huibin Chen, Li Wang, Yujing Xing, Peng Wu, Qinglong Phylogeny China RNA, Ribosomal, 16S Fresh Water DNA, Bacterial Bacterial Typing Techniques Sequence Analysis, DNA Nucleic Acid Hybridization Lysobacter Fatty Acids Base Composition Xanthomonadaceae Three Gram-stain-negative, aerobic, short rod-shaped and motile strains (FXH3W, SHGZ20W and SMYT11W) were isolated from freshwater environments in China. Comparisons based on the 16S rRNA gene sequences indicated that strains FXH3W and SHGZ20W showed the highest 16S rRNA gene sequence similarity of about 99.6% to '' MIC 1.5, and strain SMYT11W showed the highest 16S rRNA gene sequence similarity of 99.8% to D4P002, respectively. Observing the phylogenetic trees reconstructed based on the 16S rRNA gene sequences, the species of genera and were not monophyletic and often mixed together. The further reconstructed phylogenomic tree and Genome Taxonomy Database also showed that the species of both genera were polyphyletic, implying that the current taxonomic status of the species of both genera was questionable. The calculated OrthoANIu, digital DNA-DNA hybridization and average amino acid sequence identity (AAI) values supported that strains FXH3W and SHGZ20W should belong to the same novel species and strain SMYT11W should also represent an independent novel species. Combining the AAI values and phylogenomic analyses, the species of genera and should be reassigned to 12 genera (, , , , , , , , , , and ). The AAI values 69.5-76.0% were also proposed as the -specific thresholds for genus delineation. Strain SMYT11W should represent a novel species of the genus , for which the name sp. nov. (type strains SMYT11W=GDMCC 1.4275=KCTC 8304) is proposed. Strains FXH3W and SHGZ20W should represent a novel species of a new genus gen. nov., sp. nov. The type strain of the type species is FXH3W (=GDMCC 1.4096=KCTC 8154). |
| title | sp. nov. and gen. nov., sp. nov., isolated from freshwater environments in China and re-examining the taxonomic status of genera and . |
| topic | Phylogeny China RNA, Ribosomal, 16S Fresh Water DNA, Bacterial Bacterial Typing Techniques Sequence Analysis, DNA Nucleic Acid Hybridization Lysobacter Fatty Acids Base Composition Xanthomonadaceae |
| url | https://pubmed.ncbi.nlm.nih.gov/39556494/ |