Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model.
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| Format: | Artículo científico |
| Sprache: | en |
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Nature communications
2024
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| _version_ | 1868266280118648834 |
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| author | Stockinger, Alexander W Adelmann, Leonie Fahrenberger, Martin Ruta, Christine Özpolat, B Duygu Milivojev, Nadja Balavoine, Guillaume Raible, Florian |
| author_facet | Stockinger, Alexander W Adelmann, Leonie Fahrenberger, Martin Ruta, Christine Özpolat, B Duygu Milivojev, Nadja Balavoine, Guillaume Raible, Florian Stockinger, Alexander W Adelmann, Leonie Fahrenberger, Martin Ruta, Christine Özpolat, B Duygu Milivojev, Nadja Balavoine, Guillaume Raible, Florian |
| collection | PubMed - marine biology |
| contents | Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model. Stockinger, Alexander W Adelmann, Leonie Fahrenberger, Martin Ruta, Christine Özpolat, B Duygu Milivojev, Nadja Balavoine, Guillaume Raible, Florian Animals Regeneration Stem Cells Cell Lineage Polychaeta Single-Cell Analysis Regeneration of missing body parts can be observed in diverse animal phyla, but it remains unclear to which extent these capacities rely on shared or divergent principles. Research into this question requires detailed knowledge about the involved molecular and cellular principles in suitable reference models. By combining single-cell RNA sequencing and mosaic transgenesis in the marine annelid Platynereis dumerilii, we map cellular profiles and lineage restrictions during posterior regeneration. Our data reveal cell-type specific injury responses, re-expression of positional identity factors, and the re-emergence of stem cell signatures in multiple cell populations. Epidermis and mesodermal coelomic tissue produce distinct putative posterior stem cells (PSCs) in the emerging blastema. A novel mosaic transgenesis strategy reveals both developmental compartments and lineage restrictions during regenerative growth. Our work supports the notion that posterior regeneration involves dedifferentiation, and reveals molecular and mechanistic parallels between annelid and vertebrate regeneration. |
| format | Artículo científico |
| id | pubmed_39557833 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Nature communications |
| record_format | pubmed |
| spellingShingle | Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model. Stockinger, Alexander W Adelmann, Leonie Fahrenberger, Martin Ruta, Christine Özpolat, B Duygu Milivojev, Nadja Balavoine, Guillaume Raible, Florian Animals Regeneration Stem Cells Cell Lineage Polychaeta Single-Cell Analysis Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model. Stockinger, Alexander W Adelmann, Leonie Fahrenberger, Martin Ruta, Christine Özpolat, B Duygu Milivojev, Nadja Balavoine, Guillaume Raible, Florian Animals Regeneration Stem Cells Cell Lineage Polychaeta Single-Cell Analysis Regeneration of missing body parts can be observed in diverse animal phyla, but it remains unclear to which extent these capacities rely on shared or divergent principles. Research into this question requires detailed knowledge about the involved molecular and cellular principles in suitable reference models. By combining single-cell RNA sequencing and mosaic transgenesis in the marine annelid Platynereis dumerilii, we map cellular profiles and lineage restrictions during posterior regeneration. Our data reveal cell-type specific injury responses, re-expression of positional identity factors, and the re-emergence of stem cell signatures in multiple cell populations. Epidermis and mesodermal coelomic tissue produce distinct putative posterior stem cells (PSCs) in the emerging blastema. A novel mosaic transgenesis strategy reveals both developmental compartments and lineage restrictions during regenerative growth. Our work supports the notion that posterior regeneration involves dedifferentiation, and reveals molecular and mechanistic parallels between annelid and vertebrate regeneration. |
| title | Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model. |
| topic | Animals Regeneration Stem Cells Cell Lineage Polychaeta Single-Cell Analysis |
| url | https://pubmed.ncbi.nlm.nih.gov/39557833/ |