Single-cell transcriptomes reveal spermatogonial stem cells and the dynamic heterogeneity of spermatogenesis in a seasonal breeding teleost.
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| Autori principali: | , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
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Development (Cambridge, England)
2024
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| _version_ | 1868266280106065920 |
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| author | Yang, Yang Zhou, Yinan Wessel, Gary Hu, Weihua Xu, Dongdong |
| author_facet | Yang, Yang Zhou, Yinan Wessel, Gary Hu, Weihua Xu, Dongdong Yang, Yang Zhou, Yinan Wessel, Gary Hu, Weihua Xu, Dongdong |
| collection | PubMed - marine biology |
| contents | Single-cell transcriptomes reveal spermatogonial stem cells and the dynamic heterogeneity of spermatogenesis in a seasonal breeding teleost. Yang, Yang Zhou, Yinan Wessel, Gary Hu, Weihua Xu, Dongdong Animals Male Spermatogenesis Single-Cell Analysis Seasons Spermatogonia Transcriptome Testis Perciformes Adult Germline Stem Cells Stem Cells Cell Differentiation Seasonal spermatogenesis in fish is driven by spermatogonial stem cells (SSCs), which undergo a complex cellular process to differentiate into mature sperm. In this study, we characterized spermatogenesis in the large yellow croaker (Larimichthys crocea), a marine fish of significant commercial value, based on a high-resolution single-cell RNA-sequencing atlas of testicular cells from three distinct developmental stages: juvenile, adult differentiating and regressed testes. We detailed a continuous developmental trajectory of spermatogenic cells, from spermatogonia to spermatids, elucidating the molecular events involved in spermatogenesis. We uncovered dynamic heterogeneity in cellular compositions throughout the annual reproductive cycle, accompanied by strong molecular signatures within specific testicular cells. Notably, we identified a distinct population of SSCs and observed a critical metabolic transition from glycolysis to oxidative phosphorylation, enhancing our understanding of the biochemical and molecular characteristics of SSCs. Additionally, we elucidated the interactions between somatic cells and spermatogonia, illuminating the mechanisms that regulate SSC development. Overall, this work enhances our understanding of spermatogenesis in seasonal breeding teleosts and provides essential insights for the further conservation and culture of SSCs. |
| format | Artículo científico |
| id | pubmed_39565695 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Development (Cambridge, England) |
| record_format | pubmed |
| spellingShingle | Single-cell transcriptomes reveal spermatogonial stem cells and the dynamic heterogeneity of spermatogenesis in a seasonal breeding teleost. Yang, Yang Zhou, Yinan Wessel, Gary Hu, Weihua Xu, Dongdong Animals Male Spermatogenesis Single-Cell Analysis Seasons Spermatogonia Transcriptome Testis Perciformes Adult Germline Stem Cells Stem Cells Cell Differentiation Single-cell transcriptomes reveal spermatogonial stem cells and the dynamic heterogeneity of spermatogenesis in a seasonal breeding teleost. Yang, Yang Zhou, Yinan Wessel, Gary Hu, Weihua Xu, Dongdong Animals Male Spermatogenesis Single-Cell Analysis Seasons Spermatogonia Transcriptome Testis Perciformes Adult Germline Stem Cells Stem Cells Cell Differentiation Seasonal spermatogenesis in fish is driven by spermatogonial stem cells (SSCs), which undergo a complex cellular process to differentiate into mature sperm. In this study, we characterized spermatogenesis in the large yellow croaker (Larimichthys crocea), a marine fish of significant commercial value, based on a high-resolution single-cell RNA-sequencing atlas of testicular cells from three distinct developmental stages: juvenile, adult differentiating and regressed testes. We detailed a continuous developmental trajectory of spermatogenic cells, from spermatogonia to spermatids, elucidating the molecular events involved in spermatogenesis. We uncovered dynamic heterogeneity in cellular compositions throughout the annual reproductive cycle, accompanied by strong molecular signatures within specific testicular cells. Notably, we identified a distinct population of SSCs and observed a critical metabolic transition from glycolysis to oxidative phosphorylation, enhancing our understanding of the biochemical and molecular characteristics of SSCs. Additionally, we elucidated the interactions between somatic cells and spermatogonia, illuminating the mechanisms that regulate SSC development. Overall, this work enhances our understanding of spermatogenesis in seasonal breeding teleosts and provides essential insights for the further conservation and culture of SSCs. |
| title | Single-cell transcriptomes reveal spermatogonial stem cells and the dynamic heterogeneity of spermatogenesis in a seasonal breeding teleost. |
| topic | Animals Male Spermatogenesis Single-Cell Analysis Seasons Spermatogonia Transcriptome Testis Perciformes Adult Germline Stem Cells Stem Cells Cell Differentiation |
| url | https://pubmed.ncbi.nlm.nih.gov/39565695/ |