Monitoring Fish Bacterial Pathogens of Wild Fish Species From the South China Sea by Applying Next-Generation Sequencing on Gill Tissue.
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| Autores principales: | , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Journal of fish diseases
2025
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| Acceso en línea: | |
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| _version_ | 1868266277677563904 |
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| author | Zrihan, Shlomi Itay, Peleg Kroin, Yael Davidovich, Nadav Wosnick, Natascha Tchernov, Dan Koh, Xiu Pei Lau, Stanley C K Morick, Danny |
| author_facet | Zrihan, Shlomi Itay, Peleg Kroin, Yael Davidovich, Nadav Wosnick, Natascha Tchernov, Dan Koh, Xiu Pei Lau, Stanley C K Morick, Danny Zrihan, Shlomi Itay, Peleg Kroin, Yael Davidovich, Nadav Wosnick, Natascha Tchernov, Dan Koh, Xiu Pei Lau, Stanley C K Morick, Danny |
| collection | PubMed - marine biology |
| contents | Monitoring Fish Bacterial Pathogens of Wild Fish Species From the South China Sea by Applying Next-Generation Sequencing on Gill Tissue. Zrihan, Shlomi Itay, Peleg Kroin, Yael Davidovich, Nadav Wosnick, Natascha Tchernov, Dan Koh, Xiu Pei Lau, Stanley C K Morick, Danny Animals Gills High-Throughput Nucleotide Sequencing Fish Diseases Bacteria RNA, Ribosomal, 16S Microbiota China DNA, Bacterial Perciformes Fishes The classic epidemiological triangle model of host-environment-pathogen is recently being reshaped into a tetrahedron, with the growing understanding of the importance of the microbiome in this array. The gills, being a gateway into the fish body, bearing an important role in fish homeostasis, host a complex microbiome that reflects the ambient water, while also showing resemblance to gut microbiome. Next-generation sequencing (NGS) and improvements in data analysis tools enable researchers to gather and analyse a lot more data than ever before, take a closer, more detailed look at microbiota, and gain a much better understanding of the biological processes at work in these complex relations. Here, 16S rRNA amplicons of bacterial DNA extracted from the gills of 36 asymptomatic specimens of three wild fish species from the South China Sea (Nemipterus japonicus, Alepes djebaba, and Saurida tumbil) were sequenced using NGS. Data analyses revealed the presence of 20 potentially pathogenic species, including several zoonotic agents. Gill microbiota exhibited host species-specificity, and expressed a significant difference between demersal and pelagic-amphidromous fish. It is suggested that this method be more widely implemented, in order to gain more insight on ocean ecosystems' health status, as well as fish stocks of commercial importance. |
| format | Artículo científico |
| id | pubmed_39575841 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Journal of fish diseases |
| record_format | pubmed |
| spellingShingle | Monitoring Fish Bacterial Pathogens of Wild Fish Species From the South China Sea by Applying Next-Generation Sequencing on Gill Tissue. Zrihan, Shlomi Itay, Peleg Kroin, Yael Davidovich, Nadav Wosnick, Natascha Tchernov, Dan Koh, Xiu Pei Lau, Stanley C K Morick, Danny Animals Gills High-Throughput Nucleotide Sequencing Fish Diseases Bacteria RNA, Ribosomal, 16S Microbiota China DNA, Bacterial Perciformes Fishes Monitoring Fish Bacterial Pathogens of Wild Fish Species From the South China Sea by Applying Next-Generation Sequencing on Gill Tissue. Zrihan, Shlomi Itay, Peleg Kroin, Yael Davidovich, Nadav Wosnick, Natascha Tchernov, Dan Koh, Xiu Pei Lau, Stanley C K Morick, Danny Animals Gills High-Throughput Nucleotide Sequencing Fish Diseases Bacteria RNA, Ribosomal, 16S Microbiota China DNA, Bacterial Perciformes Fishes The classic epidemiological triangle model of host-environment-pathogen is recently being reshaped into a tetrahedron, with the growing understanding of the importance of the microbiome in this array. The gills, being a gateway into the fish body, bearing an important role in fish homeostasis, host a complex microbiome that reflects the ambient water, while also showing resemblance to gut microbiome. Next-generation sequencing (NGS) and improvements in data analysis tools enable researchers to gather and analyse a lot more data than ever before, take a closer, more detailed look at microbiota, and gain a much better understanding of the biological processes at work in these complex relations. Here, 16S rRNA amplicons of bacterial DNA extracted from the gills of 36 asymptomatic specimens of three wild fish species from the South China Sea (Nemipterus japonicus, Alepes djebaba, and Saurida tumbil) were sequenced using NGS. Data analyses revealed the presence of 20 potentially pathogenic species, including several zoonotic agents. Gill microbiota exhibited host species-specificity, and expressed a significant difference between demersal and pelagic-amphidromous fish. It is suggested that this method be more widely implemented, in order to gain more insight on ocean ecosystems' health status, as well as fish stocks of commercial importance. |
| title | Monitoring Fish Bacterial Pathogens of Wild Fish Species From the South China Sea by Applying Next-Generation Sequencing on Gill Tissue. |
| topic | Animals Gills High-Throughput Nucleotide Sequencing Fish Diseases Bacteria RNA, Ribosomal, 16S Microbiota China DNA, Bacterial Perciformes Fishes |
| url | https://pubmed.ncbi.nlm.nih.gov/39575841/ |