Antigenic epitopes and cross-reactivity analysis of tropomyosin from .
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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Food & function
2024
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| _version_ | 1868266277655543810 |
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| author | Ni, Ling-Na Huan, Fei Gao, Shuai Liu, Meng Wu, Ming-Xuan Gu, Yi Lai, Dong Liu, Qing-Mei Liu, Guang-Ming |
| author_facet | Ni, Ling-Na Huan, Fei Gao, Shuai Liu, Meng Wu, Ming-Xuan Gu, Yi Lai, Dong Liu, Qing-Mei Liu, Guang-Ming Ni, Ling-Na Huan, Fei Gao, Shuai Liu, Meng Wu, Ming-Xuan Gu, Yi Lai, Dong Liu, Qing-Mei Liu, Guang-Ming |
| collection | PubMed - marine biology |
| contents | Antigenic epitopes and cross-reactivity analysis of tropomyosin from . Ni, Ling-Na Huan, Fei Gao, Shuai Liu, Meng Wu, Ming-Xuan Gu, Yi Lai, Dong Liu, Qing-Mei Liu, Guang-Ming Tropomyosin Animals Cross Reactions Mice Allergens Immunoglobulin E Shellfish Hypersensitivity Epitopes, T-Lymphocyte Mice, Inbred BALB C Humans Female Epitopes, B-Lymphocyte Shellfish Epitopes Amino Acid Sequence Tropomyosin (TM) has been determined as the major allergen in . However, little information is available about its antigenic epitopes and cross-reactivity. In this study, recombinant TM was obtained through genetic engineering and its IgG-/IgE-binding activity was similar to native TM. Subsequently, four T-cell epitopes were identified by using bioinformatics technology combined with CD4 T-cell proliferation assay, and seven B-cell epitopes were identified by bioinformatics technology combined with serological assay. Moreover, dot blot analysis showed that TM had cross-reactivity among and other shellfish by using IgE polycolonal antibody from sera of shrimp-sensitive individuals or TM-immunized mice. Interestingly, three T-cell epitopes (AA, AA, and AA) and four B-cell epitopes (AA, AA, AA, and AA) of TM in were conserved between and other shellfish, which may be the main reason for the cross-reactivity between them. In summary, these findings could serve for the diagnosis and prevention of shrimp allergy. |
| format | Artículo científico |
| id | pubmed_39587923 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Food & function |
| record_format | pubmed |
| spellingShingle | Antigenic epitopes and cross-reactivity analysis of tropomyosin from . Ni, Ling-Na Huan, Fei Gao, Shuai Liu, Meng Wu, Ming-Xuan Gu, Yi Lai, Dong Liu, Qing-Mei Liu, Guang-Ming Tropomyosin Animals Cross Reactions Mice Allergens Immunoglobulin E Shellfish Hypersensitivity Epitopes, T-Lymphocyte Mice, Inbred BALB C Humans Female Epitopes, B-Lymphocyte Shellfish Epitopes Amino Acid Sequence Antigenic epitopes and cross-reactivity analysis of tropomyosin from . Ni, Ling-Na Huan, Fei Gao, Shuai Liu, Meng Wu, Ming-Xuan Gu, Yi Lai, Dong Liu, Qing-Mei Liu, Guang-Ming Tropomyosin Animals Cross Reactions Mice Allergens Immunoglobulin E Shellfish Hypersensitivity Epitopes, T-Lymphocyte Mice, Inbred BALB C Humans Female Epitopes, B-Lymphocyte Shellfish Epitopes Amino Acid Sequence Tropomyosin (TM) has been determined as the major allergen in . However, little information is available about its antigenic epitopes and cross-reactivity. In this study, recombinant TM was obtained through genetic engineering and its IgG-/IgE-binding activity was similar to native TM. Subsequently, four T-cell epitopes were identified by using bioinformatics technology combined with CD4 T-cell proliferation assay, and seven B-cell epitopes were identified by bioinformatics technology combined with serological assay. Moreover, dot blot analysis showed that TM had cross-reactivity among and other shellfish by using IgE polycolonal antibody from sera of shrimp-sensitive individuals or TM-immunized mice. Interestingly, three T-cell epitopes (AA, AA, and AA) and four B-cell epitopes (AA, AA, AA, and AA) of TM in were conserved between and other shellfish, which may be the main reason for the cross-reactivity between them. In summary, these findings could serve for the diagnosis and prevention of shrimp allergy. |
| title | Antigenic epitopes and cross-reactivity analysis of tropomyosin from . |
| topic | Tropomyosin Animals Cross Reactions Mice Allergens Immunoglobulin E Shellfish Hypersensitivity Epitopes, T-Lymphocyte Mice, Inbred BALB C Humans Female Epitopes, B-Lymphocyte Shellfish Epitopes Amino Acid Sequence |
| url | https://pubmed.ncbi.nlm.nih.gov/39587923/ |