Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Biosensors
2024
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39589997/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266277653446657 |
|---|---|
| author | Abreu, Isabel Oliveira Teixeira, Cláudia Vilarinho, Rui Rocha, A Cristina S Moreira, Joaquim Agostinho Oliva-Teles, Luís Guimarães, Laura Carvalho, António Paulo |
| author_facet | Abreu, Isabel Oliveira Teixeira, Cláudia Vilarinho, Rui Rocha, A Cristina S Moreira, Joaquim Agostinho Oliva-Teles, Luís Guimarães, Laura Carvalho, António Paulo Abreu, Isabel Oliveira Teixeira, Cláudia Vilarinho, Rui Rocha, A Cristina S Moreira, Joaquim Agostinho Oliva-Teles, Luís Guimarães, Laura Carvalho, António Paulo |
| collection | PubMed - marine biology |
| contents | Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae. Abreu, Isabel Oliveira Teixeira, Cláudia Vilarinho, Rui Rocha, A Cristina S Moreira, Joaquim Agostinho Oliva-Teles, Luís Guimarães, Laura Carvalho, António Paulo Animals Zebrafish Spectrum Analysis, Raman Larva Embryo, Nonmammalian Discriminant Analysis As a highly sensitive vibrational technique, Raman spectroscopy (RS) can provide valuable chemical and molecular data useful to characterise animal cell types, tissues and organs. As a label-free, rapid detection method, RS has been considered a valuable asset in forensics, biology and medicine. The technique has been applied to zebrafish for various purposes, including physiological, biochemical or bioaccumulation analyses. The available data point out its potential for the early diagnosis of detrimental effects elicited by toxicant exposure. Nevertheless, no baseline spectra are available for zebrafish embryos and larvae that could allow for suitable planning of toxicological assessments, comparison with toxicant-elicited spectra or mechanistic understanding of biochemical and physiological responses to the exposures. With this in mind, this work carried out a baseline characterisation of Raman spectra of zebrafish embryos and larvae throughout early development. Raman spectra were recorded from the iris, forebrain, melanocytes, heart, muscle and swim bladder between 24 and 168 h post-fertilisation. A chemometrics approach, based on partial least-squares discriminant analysis (PLS-DA), was used to obtain a Raman characterisation of each tissue or organ. In total, 117 Raman bands were identified, of which 24 were well represented and, thus, retained in the data analysed. Only three bands were found to be common to all organs and tissues. The PLS-DA provided a tentative Raman spectral fingerprint typical of each tissue or organ, reflecting the ongoing developmental dynamics. The bands showed frequencies previously assigned to collagen, cholesterol, various essential amino acids, carbohydrates and nucleic acids. |
| format | Artículo científico |
| id | pubmed_39589997 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Biosensors |
| record_format | pubmed |
| spellingShingle | Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae. Abreu, Isabel Oliveira Teixeira, Cláudia Vilarinho, Rui Rocha, A Cristina S Moreira, Joaquim Agostinho Oliva-Teles, Luís Guimarães, Laura Carvalho, António Paulo Animals Zebrafish Spectrum Analysis, Raman Larva Embryo, Nonmammalian Discriminant Analysis Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae. Abreu, Isabel Oliveira Teixeira, Cláudia Vilarinho, Rui Rocha, A Cristina S Moreira, Joaquim Agostinho Oliva-Teles, Luís Guimarães, Laura Carvalho, António Paulo Animals Zebrafish Spectrum Analysis, Raman Larva Embryo, Nonmammalian Discriminant Analysis As a highly sensitive vibrational technique, Raman spectroscopy (RS) can provide valuable chemical and molecular data useful to characterise animal cell types, tissues and organs. As a label-free, rapid detection method, RS has been considered a valuable asset in forensics, biology and medicine. The technique has been applied to zebrafish for various purposes, including physiological, biochemical or bioaccumulation analyses. The available data point out its potential for the early diagnosis of detrimental effects elicited by toxicant exposure. Nevertheless, no baseline spectra are available for zebrafish embryos and larvae that could allow for suitable planning of toxicological assessments, comparison with toxicant-elicited spectra or mechanistic understanding of biochemical and physiological responses to the exposures. With this in mind, this work carried out a baseline characterisation of Raman spectra of zebrafish embryos and larvae throughout early development. Raman spectra were recorded from the iris, forebrain, melanocytes, heart, muscle and swim bladder between 24 and 168 h post-fertilisation. A chemometrics approach, based on partial least-squares discriminant analysis (PLS-DA), was used to obtain a Raman characterisation of each tissue or organ. In total, 117 Raman bands were identified, of which 24 were well represented and, thus, retained in the data analysed. Only three bands were found to be common to all organs and tissues. The PLS-DA provided a tentative Raman spectral fingerprint typical of each tissue or organ, reflecting the ongoing developmental dynamics. The bands showed frequencies previously assigned to collagen, cholesterol, various essential amino acids, carbohydrates and nucleic acids. |
| title | Baseline Raman Spectral Fingerprints of Zebrafish Embryos and Larvae. |
| topic | Animals Zebrafish Spectrum Analysis, Raman Larva Embryo, Nonmammalian Discriminant Analysis |
| url | https://pubmed.ncbi.nlm.nih.gov/39589997/ |