Protective Effect of Marine Peptide from Against Gentamicin-Induced Hair Cell Damage in Zebrafish.
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| Natura: | Artículo científico |
| Lingua: | en |
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Marine drugs
2024
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| _version_ | 1868266275390619648 |
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| author | Zheng, Hongbao Zhu, Ranran Zhang, Yun Liu, Kechun Xia, Qing Li, Peihai Sun, Xiaoyue Sun, Chen Zhang, Shanshan |
| author_facet | Zheng, Hongbao Zhu, Ranran Zhang, Yun Liu, Kechun Xia, Qing Li, Peihai Sun, Xiaoyue Sun, Chen Zhang, Shanshan Zheng, Hongbao Zhu, Ranran Zhang, Yun Liu, Kechun Xia, Qing Li, Peihai Sun, Xiaoyue Sun, Chen Zhang, Shanshan |
| collection | PubMed - marine biology |
| contents | Protective Effect of Marine Peptide from Against Gentamicin-Induced Hair Cell Damage in Zebrafish. Zheng, Hongbao Zhu, Ranran Zhang, Yun Liu, Kechun Xia, Qing Li, Peihai Sun, Xiaoyue Sun, Chen Zhang, Shanshan Animals Zebrafish Hair Cells, Auditory Gentamicins Reactive Oxygen Species Peptides Anti-Bacterial Agents Apoptosis Hearing Loss Protective Agents Mitochondrial Permeability Transition Pore Disease Models, Animal Auditory hair cell damage induced by aminoglycoside antibiotics (AmAn) leads to hearing loss, which has a serious effect on people's mental and physical health. This ototoxicity is thought to be related with the excessive accumulation of reactive oxygen species (ROS) in hair cells. However, therapeutic agents that protect hair cells are limited. Marine peptides have been shown to have excellent potential applications in disease prevention and treatment. Therefore, this study investigated the protective effects of an active peptide from against AmAn-induced hair cell damage using the model of hair cell damage zebrafish. We identified the number, ultrastructure, and function of hair cells using fluorescence probes and scanning electron microscopy. The uptake of AmAn, ROS level, mitochondrial permeability transition pore, and apoptosis in hair cells were also tested by fluorescence labeling and TUNEL assay. The molecular mechanism for hair cell protection exerted by the peptide was detected by a real-time quantitative PCR assay. The results indicated that the peptide suppressed the uptake of AmAn but did not damage the function of hair cells mediating hearing. It also prevented ROS accumulation, decreased the occurrence of apoptosis, and rescued the abnormal opening and expressions of mitochondrial permeability transition pore and genes related to antioxidants. The peptide may be an effective therapeutic agent for AmAn-induced ototoxicity. In the future, we plan to use mammalian models to further investigate the otoprotective effect of the peptide. |
| format | Artículo científico |
| id | pubmed_39590799 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Marine drugs |
| record_format | pubmed |
| spellingShingle | Protective Effect of Marine Peptide from Against Gentamicin-Induced Hair Cell Damage in Zebrafish. Zheng, Hongbao Zhu, Ranran Zhang, Yun Liu, Kechun Xia, Qing Li, Peihai Sun, Xiaoyue Sun, Chen Zhang, Shanshan Animals Zebrafish Hair Cells, Auditory Gentamicins Reactive Oxygen Species Peptides Anti-Bacterial Agents Apoptosis Hearing Loss Protective Agents Mitochondrial Permeability Transition Pore Disease Models, Animal Protective Effect of Marine Peptide from Against Gentamicin-Induced Hair Cell Damage in Zebrafish. Zheng, Hongbao Zhu, Ranran Zhang, Yun Liu, Kechun Xia, Qing Li, Peihai Sun, Xiaoyue Sun, Chen Zhang, Shanshan Animals Zebrafish Hair Cells, Auditory Gentamicins Reactive Oxygen Species Peptides Anti-Bacterial Agents Apoptosis Hearing Loss Protective Agents Mitochondrial Permeability Transition Pore Disease Models, Animal Auditory hair cell damage induced by aminoglycoside antibiotics (AmAn) leads to hearing loss, which has a serious effect on people's mental and physical health. This ototoxicity is thought to be related with the excessive accumulation of reactive oxygen species (ROS) in hair cells. However, therapeutic agents that protect hair cells are limited. Marine peptides have been shown to have excellent potential applications in disease prevention and treatment. Therefore, this study investigated the protective effects of an active peptide from against AmAn-induced hair cell damage using the model of hair cell damage zebrafish. We identified the number, ultrastructure, and function of hair cells using fluorescence probes and scanning electron microscopy. The uptake of AmAn, ROS level, mitochondrial permeability transition pore, and apoptosis in hair cells were also tested by fluorescence labeling and TUNEL assay. The molecular mechanism for hair cell protection exerted by the peptide was detected by a real-time quantitative PCR assay. The results indicated that the peptide suppressed the uptake of AmAn but did not damage the function of hair cells mediating hearing. It also prevented ROS accumulation, decreased the occurrence of apoptosis, and rescued the abnormal opening and expressions of mitochondrial permeability transition pore and genes related to antioxidants. The peptide may be an effective therapeutic agent for AmAn-induced ototoxicity. In the future, we plan to use mammalian models to further investigate the otoprotective effect of the peptide. |
| title | Protective Effect of Marine Peptide from Against Gentamicin-Induced Hair Cell Damage in Zebrafish. |
| topic | Animals Zebrafish Hair Cells, Auditory Gentamicins Reactive Oxygen Species Peptides Anti-Bacterial Agents Apoptosis Hearing Loss Protective Agents Mitochondrial Permeability Transition Pore Disease Models, Animal |
| url | https://pubmed.ncbi.nlm.nih.gov/39590799/ |