Fungal Methane Production Under High Hydrostatic Pressure in Deep Subseafloor Sediments.
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| Main Authors: | , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Microorganisms
2024
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| _version_ | 1868266275374891010 |
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| author | Zhao, Mengshi Li, Dongxu Liu, Jie Fang, Jiasong Liu, Changhong |
| author_facet | Zhao, Mengshi Li, Dongxu Liu, Jie Fang, Jiasong Liu, Changhong Zhao, Mengshi Li, Dongxu Liu, Jie Fang, Jiasong Liu, Changhong |
| collection | PubMed - marine biology |
| contents | Fungal Methane Production Under High Hydrostatic Pressure in Deep Subseafloor Sediments. Zhao, Mengshi Li, Dongxu Liu, Jie Fang, Jiasong Liu, Changhong Fungi inhabiting deep subseafloor sediments have been shown to possess anaerobic methane (CH) production capabilities under atmospheric conditions. However, their ability to produce CH under in situ conditions with high hydrostatic pressure (HHP) remains unclear. Here, 20R-7-F01, isolated from ~2 km below the seafloor, was cultured in Seawater Medium (SM) in culture bottles fitted with sterile syringes for pressure equilibration. Subsequently, these culture bottles were transferred into 1 L stainless steel pressure vessels at 30 °C for 5 days to simulate in situ HHP and anaerobic environments. Our comprehensive analysis of bioactivity, biomass, and transcriptomics revealed that the not only survived but significantly enhanced CH production, reaching approximately 2.5 times higher levels under 35 MPa HHP compared to 0.1 MPa standard atmospheric pressure. Pathways associated with carbohydrate metabolism, methylation, hydrolase activity, cysteine and methionine metabolism, and oxidoreductase activity were notably activated under HHP. Specifically, key genes involved in fungal anaerobic CH synthesis, including methyltransferase mct1 and dehalogenase dh3, were upregulated 7.9- and 12.5-fold, respectively, under HHP. Enhanced CH production under HHP was primarily attributed to oxidative stress induced by pressure, supported by intracellular reactive oxygen species (ROS) levels and comparative treatments with cadmium chloride and hydrogen peroxide. These results may provide a strong theoretical basis and practical guidance for future studies on the contribution of fungi to global CH flux. |
| format | Artículo científico |
| id | pubmed_39597547 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Microorganisms |
| record_format | pubmed |
| spellingShingle | Fungal Methane Production Under High Hydrostatic Pressure in Deep Subseafloor Sediments. Zhao, Mengshi Li, Dongxu Liu, Jie Fang, Jiasong Liu, Changhong Fungal Methane Production Under High Hydrostatic Pressure in Deep Subseafloor Sediments. Zhao, Mengshi Li, Dongxu Liu, Jie Fang, Jiasong Liu, Changhong Fungi inhabiting deep subseafloor sediments have been shown to possess anaerobic methane (CH) production capabilities under atmospheric conditions. However, their ability to produce CH under in situ conditions with high hydrostatic pressure (HHP) remains unclear. Here, 20R-7-F01, isolated from ~2 km below the seafloor, was cultured in Seawater Medium (SM) in culture bottles fitted with sterile syringes for pressure equilibration. Subsequently, these culture bottles were transferred into 1 L stainless steel pressure vessels at 30 °C for 5 days to simulate in situ HHP and anaerobic environments. Our comprehensive analysis of bioactivity, biomass, and transcriptomics revealed that the not only survived but significantly enhanced CH production, reaching approximately 2.5 times higher levels under 35 MPa HHP compared to 0.1 MPa standard atmospheric pressure. Pathways associated with carbohydrate metabolism, methylation, hydrolase activity, cysteine and methionine metabolism, and oxidoreductase activity were notably activated under HHP. Specifically, key genes involved in fungal anaerobic CH synthesis, including methyltransferase mct1 and dehalogenase dh3, were upregulated 7.9- and 12.5-fold, respectively, under HHP. Enhanced CH production under HHP was primarily attributed to oxidative stress induced by pressure, supported by intracellular reactive oxygen species (ROS) levels and comparative treatments with cadmium chloride and hydrogen peroxide. These results may provide a strong theoretical basis and practical guidance for future studies on the contribution of fungi to global CH flux. |
| title | Fungal Methane Production Under High Hydrostatic Pressure in Deep Subseafloor Sediments. |
| url | https://pubmed.ncbi.nlm.nih.gov/39597547/ |