Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain sp. DH-2.
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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Microorganisms
2024
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| _version_ | 1868266275374891008 |
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| author | Luo, Zetian Ma, Jiajun Huang, Lei Li, Dahui Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong |
| author_facet | Luo, Zetian Ma, Jiajun Huang, Lei Li, Dahui Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong Luo, Zetian Ma, Jiajun Huang, Lei Li, Dahui Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong |
| collection | PubMed - marine biology |
| contents | Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain sp. DH-2. Luo, Zetian Ma, Jiajun Huang, Lei Li, Dahui Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong Aniline and crude oil are common environmental pollutants that present a significant risk to both the ecological and human health environments. The implementation of efficacious bioremediation strategies is imperative for the elimination of these contaminants. In this study, a bacterial strain designated DH-2 was isolated from soil contaminated with aniline. The strain was identified as belonging to the genus . The optimal conditions for the growth and aniline degradation by strain DH-2 were determined to be pH 8.0 and 35 °C, respectively. Under these conditions, the degradation rate of aniline at a concentration of 1000 mg/L exceeded 90% within 36 h. Even in the presence of 4% NaCl, the degradation rate remained above 60%. HPLC-MS analysis revealed that the aniline degradation pathway of strain DH-2 follows the catechol pathway. Additionally, strain DH-2 is capable of utilizing crude oil as the sole carbon source, achieving a degradation rate of 91.0% for 2% crude oil concentration within 4 days. In soil modeling experiments, strain DH-2 was observed to degrade aniline and crude oil under triple stress conditions, including 1000 mg/L aniline, 2% crude oil, and 20 mg/L Fe(II) or Pb(II). Complete degradation of aniline and crude oil was achieved after 3 days and 12 days, respectively. The addition of Fe(II) or Pb(II) ions was found to enhance the degradation ability of DH-2. These results demonstrate that strain DH-2 is an extremely effective biodegradable strain, with potential applications in the remediation of environments contaminated with aniline and crude oil, even in the presence of heavy metals. |
| format | Artículo científico |
| id | pubmed_39597682 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Microorganisms |
| record_format | pubmed |
| spellingShingle | Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain sp. DH-2. Luo, Zetian Ma, Jiajun Huang, Lei Li, Dahui Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain sp. DH-2. Luo, Zetian Ma, Jiajun Huang, Lei Li, Dahui Gao, Guohui Zhao, Yihe Antunes, Agostinho Li, Meitong Aniline and crude oil are common environmental pollutants that present a significant risk to both the ecological and human health environments. The implementation of efficacious bioremediation strategies is imperative for the elimination of these contaminants. In this study, a bacterial strain designated DH-2 was isolated from soil contaminated with aniline. The strain was identified as belonging to the genus . The optimal conditions for the growth and aniline degradation by strain DH-2 were determined to be pH 8.0 and 35 °C, respectively. Under these conditions, the degradation rate of aniline at a concentration of 1000 mg/L exceeded 90% within 36 h. Even in the presence of 4% NaCl, the degradation rate remained above 60%. HPLC-MS analysis revealed that the aniline degradation pathway of strain DH-2 follows the catechol pathway. Additionally, strain DH-2 is capable of utilizing crude oil as the sole carbon source, achieving a degradation rate of 91.0% for 2% crude oil concentration within 4 days. In soil modeling experiments, strain DH-2 was observed to degrade aniline and crude oil under triple stress conditions, including 1000 mg/L aniline, 2% crude oil, and 20 mg/L Fe(II) or Pb(II). Complete degradation of aniline and crude oil was achieved after 3 days and 12 days, respectively. The addition of Fe(II) or Pb(II) ions was found to enhance the degradation ability of DH-2. These results demonstrate that strain DH-2 is an extremely effective biodegradable strain, with potential applications in the remediation of environments contaminated with aniline and crude oil, even in the presence of heavy metals. |
| title | Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain sp. DH-2. |
| url | https://pubmed.ncbi.nlm.nih.gov/39597682/ |