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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
bioRxiv : the preprint server for biology
2024
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39605611/ |
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| author | Zahnow, Fynn Jäger, Chiara Mohamed, Yassmin Vogelhuber, Gianluca May, Fabian Ciocan, Alexandra Maria Manieri, Arianna Maxeiner, Stephan Krasteva-Christ, Gabriela Cobain, Matthew R D Podsiadlowski, Lars Crespo-Picazo, José Luis García-Párraga, Daniel Althaus, Mike |
| author_facet | Zahnow, Fynn Jäger, Chiara Mohamed, Yassmin Vogelhuber, Gianluca May, Fabian Ciocan, Alexandra Maria Manieri, Arianna Maxeiner, Stephan Krasteva-Christ, Gabriela Cobain, Matthew R D Podsiadlowski, Lars Crespo-Picazo, José Luis García-Párraga, Daniel Althaus, Mike Zahnow, Fynn Jäger, Chiara Mohamed, Yassmin Vogelhuber, Gianluca May, Fabian Ciocan, Alexandra Maria Manieri, Arianna Maxeiner, Stephan Krasteva-Christ, Gabriela Cobain, Matthew R D Podsiadlowski, Lars Crespo-Picazo, José Luis García-Párraga, Daniel Althaus, Mike |
| collection | PubMed - marine biology |
| contents | The evolutionary path of the epithelial sodium channel δ-subunit in Cetartiodactyla points to a role in sodium sensing. Zahnow, Fynn Jäger, Chiara Mohamed, Yassmin Vogelhuber, Gianluca May, Fabian Ciocan, Alexandra Maria Manieri, Arianna Maxeiner, Stephan Krasteva-Christ, Gabriela Cobain, Matthew R D Podsiadlowski, Lars Crespo-Picazo, José Luis García-Párraga, Daniel Althaus, Mike The epithelial sodium channel (ENaC) plays a key role in osmoregulation in tetrapod vertebrates and is a candidate receptor for salt taste sensation. There are four ENaC subunits (α, β, γ, δ) which form αβγ- or δβγ ENaCs. While αβγ-ENaC is a 'maintenance protein' controlling sodium and potassium homeostasis, δβγ-ENaC might represent a 'stress protein' monitoring high sodium concentrations. The δ-subunit emerged with water-to-land transition of tetrapod vertebrate ancestors. We investigated the evolutionary path of ENaC-coding genes in Cetartiodactyla, a group comprising even-toed ungulates and the cetaceans (whales/dolphins) which transitioned from terrestrial to marine environments in the Eocene. The genes (α-ENaC), (β-ENaC) and (γ-ENaC) are intact in all 22 investigated cetartiodactylan families. While (δ-ENaC) is intact in terrestrial Artiodactyla, it is a pseudogene in 12 cetacean families. A fusion of exons 11 and 12 under preservation of the open reading frame was observed in the Antilopinae, representing a new feature of this clade. Transcripts of and were present in kidney and lung tissues of Bottlenose dolphins, highlighting αβγ-ENaC's role as a maintenance protein. Consistent with loss, Bottlenose dolphins and Beluga whales did not show behavioural differences to stimuli with or without sodium in seawater-equivalent concentrations. These data suggest a function of δ-ENaC as a sodium sensing protein which might have become obsolete in cetaceans after the migration to high-salinity marine environments. Consistently, there is reduced selection pressure or pseudogenisation of in other marine mammals, including sirenians, pinnipeds and sea otter. |
| format | Artículo científico |
| id | pubmed_39605611 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | bioRxiv : the preprint server for biology |
| record_format | pubmed |
| spellingShingle | The evolutionary path of the epithelial sodium channel δ-subunit in Cetartiodactyla points to a role in sodium sensing. Zahnow, Fynn Jäger, Chiara Mohamed, Yassmin Vogelhuber, Gianluca May, Fabian Ciocan, Alexandra Maria Manieri, Arianna Maxeiner, Stephan Krasteva-Christ, Gabriela Cobain, Matthew R D Podsiadlowski, Lars Crespo-Picazo, José Luis García-Párraga, Daniel Althaus, Mike The evolutionary path of the epithelial sodium channel δ-subunit in Cetartiodactyla points to a role in sodium sensing. Zahnow, Fynn Jäger, Chiara Mohamed, Yassmin Vogelhuber, Gianluca May, Fabian Ciocan, Alexandra Maria Manieri, Arianna Maxeiner, Stephan Krasteva-Christ, Gabriela Cobain, Matthew R D Podsiadlowski, Lars Crespo-Picazo, José Luis García-Párraga, Daniel Althaus, Mike The epithelial sodium channel (ENaC) plays a key role in osmoregulation in tetrapod vertebrates and is a candidate receptor for salt taste sensation. There are four ENaC subunits (α, β, γ, δ) which form αβγ- or δβγ ENaCs. While αβγ-ENaC is a 'maintenance protein' controlling sodium and potassium homeostasis, δβγ-ENaC might represent a 'stress protein' monitoring high sodium concentrations. The δ-subunit emerged with water-to-land transition of tetrapod vertebrate ancestors. We investigated the evolutionary path of ENaC-coding genes in Cetartiodactyla, a group comprising even-toed ungulates and the cetaceans (whales/dolphins) which transitioned from terrestrial to marine environments in the Eocene. The genes (α-ENaC), (β-ENaC) and (γ-ENaC) are intact in all 22 investigated cetartiodactylan families. While (δ-ENaC) is intact in terrestrial Artiodactyla, it is a pseudogene in 12 cetacean families. A fusion of exons 11 and 12 under preservation of the open reading frame was observed in the Antilopinae, representing a new feature of this clade. Transcripts of and were present in kidney and lung tissues of Bottlenose dolphins, highlighting αβγ-ENaC's role as a maintenance protein. Consistent with loss, Bottlenose dolphins and Beluga whales did not show behavioural differences to stimuli with or without sodium in seawater-equivalent concentrations. These data suggest a function of δ-ENaC as a sodium sensing protein which might have become obsolete in cetaceans after the migration to high-salinity marine environments. Consistently, there is reduced selection pressure or pseudogenisation of in other marine mammals, including sirenians, pinnipeds and sea otter. |
| title | The evolutionary path of the epithelial sodium channel δ-subunit in Cetartiodactyla points to a role in sodium sensing. |
| url | https://pubmed.ncbi.nlm.nih.gov/39605611/ |