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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Food chemistry
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39612832/ |
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| _version_ | 1868266275352870913 |
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| author | Wei, Kaiyue Zhang, Ling Li, Nan Gao, Kunpeng Li, Xuehan Li, Jiao Wang, Sai Mao, Xiangzhao |
| author_facet | Wei, Kaiyue Zhang, Ling Li, Nan Gao, Kunpeng Li, Xuehan Li, Jiao Wang, Sai Mao, Xiangzhao Wei, Kaiyue Zhang, Ling Li, Nan Gao, Kunpeng Li, Xuehan Li, Jiao Wang, Sai Mao, Xiangzhao |
| collection | PubMed - marine biology |
| contents | A colorimetric biosensor composed of split aptamers and mannan oligosaccharide nanozyme to monitor synthetic His-tagged food biomolecules. Wei, Kaiyue Zhang, Ling Li, Nan Gao, Kunpeng Li, Xuehan Li, Jiao Wang, Sai Mao, Xiangzhao Biosensing Techniques Colorimetry Mannans Aptamers, Nucleotide Oligosaccharides Gold Metal Nanoparticles Histidine Food synthetic biology is garnering increasing attention for its potential to generate bioactive components. His-tag is one of the most popular tags used in food synthetic biology. Herein, His-tag, His-tagged proteins, and His-tagged peptides were adopted as the model targets, and a commonly used biosensor was developed to monitor His-tagged food biomolecules, using split aptamers as specific recognition probes and nanozyme as the transduction element. A strategy to generate high-affinity split aptamers was proposed, obtaining a pair of split aptamers for His-tag (K = 132 nM). AuNPs-mannan oligosaccharide nanozyme was fabricated and combined with the split aptamers to develop the biosensor. The functional mechanism of the probes and the nanozyme was revealed. The biosensor demonstrated good sensitivity, selectivity, and practicability when analyzing synthetic His-tagged proteins and peptides in real-world samples, with a limit of detection of 12.44 nM. The strategies provide robust reference for developing analytical methods for synthetic biomolecules. |
| format | Artículo científico |
| id | pubmed_39612832 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Food chemistry |
| record_format | pubmed |
| spellingShingle | A colorimetric biosensor composed of split aptamers and mannan oligosaccharide nanozyme to monitor synthetic His-tagged food biomolecules. Wei, Kaiyue Zhang, Ling Li, Nan Gao, Kunpeng Li, Xuehan Li, Jiao Wang, Sai Mao, Xiangzhao Biosensing Techniques Colorimetry Mannans Aptamers, Nucleotide Oligosaccharides Gold Metal Nanoparticles Histidine A colorimetric biosensor composed of split aptamers and mannan oligosaccharide nanozyme to monitor synthetic His-tagged food biomolecules. Wei, Kaiyue Zhang, Ling Li, Nan Gao, Kunpeng Li, Xuehan Li, Jiao Wang, Sai Mao, Xiangzhao Biosensing Techniques Colorimetry Mannans Aptamers, Nucleotide Oligosaccharides Gold Metal Nanoparticles Histidine Food synthetic biology is garnering increasing attention for its potential to generate bioactive components. His-tag is one of the most popular tags used in food synthetic biology. Herein, His-tag, His-tagged proteins, and His-tagged peptides were adopted as the model targets, and a commonly used biosensor was developed to monitor His-tagged food biomolecules, using split aptamers as specific recognition probes and nanozyme as the transduction element. A strategy to generate high-affinity split aptamers was proposed, obtaining a pair of split aptamers for His-tag (K = 132 nM). AuNPs-mannan oligosaccharide nanozyme was fabricated and combined with the split aptamers to develop the biosensor. The functional mechanism of the probes and the nanozyme was revealed. The biosensor demonstrated good sensitivity, selectivity, and practicability when analyzing synthetic His-tagged proteins and peptides in real-world samples, with a limit of detection of 12.44 nM. The strategies provide robust reference for developing analytical methods for synthetic biomolecules. |
| title | A colorimetric biosensor composed of split aptamers and mannan oligosaccharide nanozyme to monitor synthetic His-tagged food biomolecules. |
| topic | Biosensing Techniques Colorimetry Mannans Aptamers, Nucleotide Oligosaccharides Gold Metal Nanoparticles Histidine |
| url | https://pubmed.ncbi.nlm.nih.gov/39612832/ |