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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Microbial pathogenesis
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39615708/ |
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| _version_ | 1868266272982040576 |
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| author | Diya, D V Dominic Muneer, A Linu, Eldho Sajeevan, T P Jayesh, Puthumana Joseph, Valsamma Philip, Rosamma Singh, I S Bright |
| author_facet | Diya, D V Dominic Muneer, A Linu, Eldho Sajeevan, T P Jayesh, Puthumana Joseph, Valsamma Philip, Rosamma Singh, I S Bright Diya, D V Dominic Muneer, A Linu, Eldho Sajeevan, T P Jayesh, Puthumana Joseph, Valsamma Philip, Rosamma Singh, I S Bright |
| collection | PubMed - marine biology |
| contents | Replication kinetics, morphogenesis and interaction of shrimp parvovirus Penaeus stylirostris penstyldensovirus (PstDV1) in PmLyO-Sf9 cells. Diya, D V Dominic Muneer, A Linu, Eldho Sajeevan, T P Jayesh, Puthumana Joseph, Valsamma Philip, Rosamma Singh, I S Bright Animals Virus Replication Penaeidae Sf9 Cells Virus Internalization Capsid Proteins Kinetics Cell Line Host-Pathogen Interactions Cell Cycle Penaeus stylirostris penstyldensovirus (PstDV1) is one of the significant shrimp parvovirus which causes runt deformity syndrome in shrimps. In the current study, we attempted to elucidate the replication cycle of the virus in PmLyO-Sf9 cells. PstDV1 needs 4-5 h to complete replication in the cell line and release. Viral capsid gene expression leads to three peaks during the time course of the study at 4hpi, 12hpi and 72hpi. Immediate release of virus from the cell and reinfection in the cells observed. Calveloe mediated entry of virus to cells was noted through endosomal inhibition assay. Electron micrographs supported the findings which also depicted the receptor mediated entry, endosomal transport, viroplasm showing active virus replication and assembly of virus inside the nucleus to be released through cell lysis and occlusion bodies. Also, changes in cell cycle to make the cells to enter S phase was observed in cell cycle related genes and flow cytometric analysis. This reveal the potency of the cell line to study host-pathogen interaction, viral morphogenesis which could promotes successful development of antiviral formulations against the virus while preserving the environment for future generations. |
| format | Artículo científico |
| id | pubmed_39615708 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Microbial pathogenesis |
| record_format | pubmed |
| spellingShingle | Replication kinetics, morphogenesis and interaction of shrimp parvovirus Penaeus stylirostris penstyldensovirus (PstDV1) in PmLyO-Sf9 cells. Diya, D V Dominic Muneer, A Linu, Eldho Sajeevan, T P Jayesh, Puthumana Joseph, Valsamma Philip, Rosamma Singh, I S Bright Animals Virus Replication Penaeidae Sf9 Cells Virus Internalization Capsid Proteins Kinetics Cell Line Host-Pathogen Interactions Cell Cycle Replication kinetics, morphogenesis and interaction of shrimp parvovirus Penaeus stylirostris penstyldensovirus (PstDV1) in PmLyO-Sf9 cells. Diya, D V Dominic Muneer, A Linu, Eldho Sajeevan, T P Jayesh, Puthumana Joseph, Valsamma Philip, Rosamma Singh, I S Bright Animals Virus Replication Penaeidae Sf9 Cells Virus Internalization Capsid Proteins Kinetics Cell Line Host-Pathogen Interactions Cell Cycle Penaeus stylirostris penstyldensovirus (PstDV1) is one of the significant shrimp parvovirus which causes runt deformity syndrome in shrimps. In the current study, we attempted to elucidate the replication cycle of the virus in PmLyO-Sf9 cells. PstDV1 needs 4-5 h to complete replication in the cell line and release. Viral capsid gene expression leads to three peaks during the time course of the study at 4hpi, 12hpi and 72hpi. Immediate release of virus from the cell and reinfection in the cells observed. Calveloe mediated entry of virus to cells was noted through endosomal inhibition assay. Electron micrographs supported the findings which also depicted the receptor mediated entry, endosomal transport, viroplasm showing active virus replication and assembly of virus inside the nucleus to be released through cell lysis and occlusion bodies. Also, changes in cell cycle to make the cells to enter S phase was observed in cell cycle related genes and flow cytometric analysis. This reveal the potency of the cell line to study host-pathogen interaction, viral morphogenesis which could promotes successful development of antiviral formulations against the virus while preserving the environment for future generations. |
| title | Replication kinetics, morphogenesis and interaction of shrimp parvovirus Penaeus stylirostris penstyldensovirus (PstDV1) in PmLyO-Sf9 cells. |
| topic | Animals Virus Replication Penaeidae Sf9 Cells Virus Internalization Capsid Proteins Kinetics Cell Line Host-Pathogen Interactions Cell Cycle |
| url | https://pubmed.ncbi.nlm.nih.gov/39615708/ |