Carboxymethylated yeast β-glucan: Biological activity screening in zebrafish, sprayable hydrogel preparation, and wound healing study in diabetic mice.
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| Autori principali: | , , , , , , , , , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
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International journal of biological macromolecules
2025
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| _version_ | 1868266272980992002 |
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| author | Xu, Chunhua Zhang, Yongchun Li, Hui Zhang, Zhihan Sheng, Wenlong Zhang, Shanshan Li, Peihai Zhang, Xuanming Li, Xiaobin Lin, Houwen Duan, Hongdong Guan, Shibing Wang, Lizhen |
| author_facet | Xu, Chunhua Zhang, Yongchun Li, Hui Zhang, Zhihan Sheng, Wenlong Zhang, Shanshan Li, Peihai Zhang, Xuanming Li, Xiaobin Lin, Houwen Duan, Hongdong Guan, Shibing Wang, Lizhen Xu, Chunhua Zhang, Yongchun Li, Hui Zhang, Zhihan Sheng, Wenlong Zhang, Shanshan Li, Peihai Zhang, Xuanming Li, Xiaobin Lin, Houwen Duan, Hongdong Guan, Shibing Wang, Lizhen |
| collection | PubMed - marine biology |
| contents | Carboxymethylated yeast β-glucan: Biological activity screening in zebrafish, sprayable hydrogel preparation, and wound healing study in diabetic mice. Xu, Chunhua Zhang, Yongchun Li, Hui Zhang, Zhihan Sheng, Wenlong Zhang, Shanshan Li, Peihai Zhang, Xuanming Li, Xiaobin Lin, Houwen Duan, Hongdong Guan, Shibing Wang, Lizhen Animals Wound Healing Zebrafish beta-Glucans Mice Diabetes Mellitus, Experimental Hydrogels Antioxidants Anti-Inflammatory Agents Cell Movement Male Saccharomyces cerevisiae Yeast β-glucan exhibits dramatic potential as wound healing regent owing to its various biological properties including immunomodulatory, anti-inflammatory, and antioxidant. But, the poor water solubility of yeast β-glucan limits its application. In this study, some carboxymethylated yeast β-glucans (CMGs) with different substitution degree was prepared. The effect of substitution degree on biological activities of carboxymethylated yeast β-glucan was investigated using zebrafish model. CMG with substitution degree 0.55 showed anti-inflammatory, antioxidant, and immunomodulatory activities in zebrafish. CMG also showed potential ability to promote angiogenesis and caudal fin regeneration. The cytotoxicity of CMG was investigated on L929 cells and the effect of CMG on cell migration was studied by scratch test. The hemolysis ratio of CMG was determined, and the in vitro antioxidant activity was studied. Next, CMG was used to prepare a sprayable hydrogel through a simple method, and the wound healing ability was studied using a streptozotocin-induced diabetic mice model. The results indicated that CMG-based sprayable hydrogel could accelerate wound healing in a diabetic mice and influence the expression of biomarkers related to inflammatory, macrophage polarization, and angiogenesis. |
| format | Artículo científico |
| id | pubmed_39615714 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of biological macromolecules |
| record_format | pubmed |
| spellingShingle | Carboxymethylated yeast β-glucan: Biological activity screening in zebrafish, sprayable hydrogel preparation, and wound healing study in diabetic mice. Xu, Chunhua Zhang, Yongchun Li, Hui Zhang, Zhihan Sheng, Wenlong Zhang, Shanshan Li, Peihai Zhang, Xuanming Li, Xiaobin Lin, Houwen Duan, Hongdong Guan, Shibing Wang, Lizhen Animals Wound Healing Zebrafish beta-Glucans Mice Diabetes Mellitus, Experimental Hydrogels Antioxidants Anti-Inflammatory Agents Cell Movement Male Saccharomyces cerevisiae Carboxymethylated yeast β-glucan: Biological activity screening in zebrafish, sprayable hydrogel preparation, and wound healing study in diabetic mice. Xu, Chunhua Zhang, Yongchun Li, Hui Zhang, Zhihan Sheng, Wenlong Zhang, Shanshan Li, Peihai Zhang, Xuanming Li, Xiaobin Lin, Houwen Duan, Hongdong Guan, Shibing Wang, Lizhen Animals Wound Healing Zebrafish beta-Glucans Mice Diabetes Mellitus, Experimental Hydrogels Antioxidants Anti-Inflammatory Agents Cell Movement Male Saccharomyces cerevisiae Yeast β-glucan exhibits dramatic potential as wound healing regent owing to its various biological properties including immunomodulatory, anti-inflammatory, and antioxidant. But, the poor water solubility of yeast β-glucan limits its application. In this study, some carboxymethylated yeast β-glucans (CMGs) with different substitution degree was prepared. The effect of substitution degree on biological activities of carboxymethylated yeast β-glucan was investigated using zebrafish model. CMG with substitution degree 0.55 showed anti-inflammatory, antioxidant, and immunomodulatory activities in zebrafish. CMG also showed potential ability to promote angiogenesis and caudal fin regeneration. The cytotoxicity of CMG was investigated on L929 cells and the effect of CMG on cell migration was studied by scratch test. The hemolysis ratio of CMG was determined, and the in vitro antioxidant activity was studied. Next, CMG was used to prepare a sprayable hydrogel through a simple method, and the wound healing ability was studied using a streptozotocin-induced diabetic mice model. The results indicated that CMG-based sprayable hydrogel could accelerate wound healing in a diabetic mice and influence the expression of biomarkers related to inflammatory, macrophage polarization, and angiogenesis. |
| title | Carboxymethylated yeast β-glucan: Biological activity screening in zebrafish, sprayable hydrogel preparation, and wound healing study in diabetic mice. |
| topic | Animals Wound Healing Zebrafish beta-Glucans Mice Diabetes Mellitus, Experimental Hydrogels Antioxidants Anti-Inflammatory Agents Cell Movement Male Saccharomyces cerevisiae |
| url | https://pubmed.ncbi.nlm.nih.gov/39615714/ |