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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Free radical biology & medicine
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39617215/ |
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| _version_ | 1868266272978894848 |
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| author | Zuccarotto, Annalisa Sollitto, Marco Leclère, Lucas Panzella, Lucia Gerdol, Marco Leone, Serena Castellano, Immacolata |
| author_facet | Zuccarotto, Annalisa Sollitto, Marco Leclère, Lucas Panzella, Lucia Gerdol, Marco Leone, Serena Castellano, Immacolata Zuccarotto, Annalisa Sollitto, Marco Leclère, Lucas Panzella, Lucia Gerdol, Marco Leone, Serena Castellano, Immacolata |
| collection | PubMed - marine biology |
| contents | Molecular evolution of ovothiol biosynthesis in animal life reveals diversity of the natural antioxidant ovothiols in Cnidaria. Zuccarotto, Annalisa Sollitto, Marco Leclère, Lucas Panzella, Lucia Gerdol, Marco Leone, Serena Castellano, Immacolata Animals Antioxidants Evolution, Molecular Cnidaria Phylogeny Transcriptome Sulfoxide synthase OvoA is the key enzyme involved in the biosynthesis of ovothiols (OSHs), secondary metabolites endowed with unique antioxidant properties. Understanding the evolution of such enzymes and the diversity of their metabolites should reveal fundamental mechanisms governing redox signaling and environmental adaptation. "Early-branching" animals such as Cnidaria display unique molecular diversity and symbiotic relationships responsible for the biosynthesis of natural products, however, they have been neglected in previous research on antioxidants and OSHs. In this work, we have integrated genome and transcriptome mining with biochemical analyses to study the evolution and diversification of OSHs biosynthesis in cnidarians. By tracing the history of the ovoA gene, we inferred its loss in the latest common ancestor of Medusozoa, followed by the acquisition of a unique ovoB/ovoA chimaeric gene in Hydrozoa, likely through a horizontal gene transfer from dinoflagellate donors. While Anthozoa (corals and anemones), bearing canonical ovoA genes, produced a striking variety of OSHs (A, B, and C), the multifunctional enzyme in Hydrozoa was related to OSH B biosynthesis, as shown in Clytia hemisphaerica. Surprisingly, the ovoA-lacking jellyfish Aurelia aurita and Pelagia noctiluca also displayed OSHs, and we provided evidence of their incorporation from external sources. Finally, transcriptome mining revealed ovoA conserved expression pattern during larval development from Cnidaria to more evolved organisms and its regulation by external stimuli, such as UV exposure. The results of our study shed light on the origin and diversification of OSH biosynthesis in basal animals and highlight the importance of redox-active molecules from ancient metazoans as cnidarians to vertebrates. |
| format | Artículo científico |
| id | pubmed_39617215 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Free radical biology & medicine |
| record_format | pubmed |
| spellingShingle | Molecular evolution of ovothiol biosynthesis in animal life reveals diversity of the natural antioxidant ovothiols in Cnidaria. Zuccarotto, Annalisa Sollitto, Marco Leclère, Lucas Panzella, Lucia Gerdol, Marco Leone, Serena Castellano, Immacolata Animals Antioxidants Evolution, Molecular Cnidaria Phylogeny Transcriptome Molecular evolution of ovothiol biosynthesis in animal life reveals diversity of the natural antioxidant ovothiols in Cnidaria. Zuccarotto, Annalisa Sollitto, Marco Leclère, Lucas Panzella, Lucia Gerdol, Marco Leone, Serena Castellano, Immacolata Animals Antioxidants Evolution, Molecular Cnidaria Phylogeny Transcriptome Sulfoxide synthase OvoA is the key enzyme involved in the biosynthesis of ovothiols (OSHs), secondary metabolites endowed with unique antioxidant properties. Understanding the evolution of such enzymes and the diversity of their metabolites should reveal fundamental mechanisms governing redox signaling and environmental adaptation. "Early-branching" animals such as Cnidaria display unique molecular diversity and symbiotic relationships responsible for the biosynthesis of natural products, however, they have been neglected in previous research on antioxidants and OSHs. In this work, we have integrated genome and transcriptome mining with biochemical analyses to study the evolution and diversification of OSHs biosynthesis in cnidarians. By tracing the history of the ovoA gene, we inferred its loss in the latest common ancestor of Medusozoa, followed by the acquisition of a unique ovoB/ovoA chimaeric gene in Hydrozoa, likely through a horizontal gene transfer from dinoflagellate donors. While Anthozoa (corals and anemones), bearing canonical ovoA genes, produced a striking variety of OSHs (A, B, and C), the multifunctional enzyme in Hydrozoa was related to OSH B biosynthesis, as shown in Clytia hemisphaerica. Surprisingly, the ovoA-lacking jellyfish Aurelia aurita and Pelagia noctiluca also displayed OSHs, and we provided evidence of their incorporation from external sources. Finally, transcriptome mining revealed ovoA conserved expression pattern during larval development from Cnidaria to more evolved organisms and its regulation by external stimuli, such as UV exposure. The results of our study shed light on the origin and diversification of OSH biosynthesis in basal animals and highlight the importance of redox-active molecules from ancient metazoans as cnidarians to vertebrates. |
| title | Molecular evolution of ovothiol biosynthesis in animal life reveals diversity of the natural antioxidant ovothiols in Cnidaria. |
| topic | Animals Antioxidants Evolution, Molecular Cnidaria Phylogeny Transcriptome |
| url | https://pubmed.ncbi.nlm.nih.gov/39617215/ |