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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
PloS one
2024
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39637050/ |
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| _version_ | 1868266272951631874 |
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| author | Wilson, Elizabeth J Barts, Nick Coffin, John L Johnson, James B Rodríguez Peña, Carlos M Kelley, Joanna L Tobler, Michael Greenway, Ryan |
| author_facet | Wilson, Elizabeth J Barts, Nick Coffin, John L Johnson, James B Rodríguez Peña, Carlos M Kelley, Joanna L Tobler, Michael Greenway, Ryan Wilson, Elizabeth J Barts, Nick Coffin, John L Johnson, James B Rodríguez Peña, Carlos M Kelley, Joanna L Tobler, Michael Greenway, Ryan |
| collection | PubMed - marine biology |
| contents | Gene expression signatures between Limia perugiae (Poeciliidae) populations from freshwater and hypersaline habitats, with comparisons to other teleosts. Wilson, Elizabeth J Barts, Nick Coffin, John L Johnson, James B Rodríguez Peña, Carlos M Kelley, Joanna L Tobler, Michael Greenway, Ryan Fishes Fresh Water Salinity Saline Waters Animal Distribution RNA-Seq Salt Tolerance Homeostasis Ion Transport Protein Folding Transcriptome Evolution, Molecular Gene Expression Regulation Osmoregulation Female Animals Gills Ecosystem Fish Proteins Salinity gradients act as strong environmental barriers that limit the distribution of aquatic organisms. Changes in gene expression associated with transitions between freshwater and saltwater environments can provide insights into organismal responses to variation in salinity. We used RNA-sequencing (RNA-seq) to investigate genome-wide variation in gene expression between a hypersaline population and a freshwater population of the livebearing fish species Limia perugiae (Poeciliidae). Our analyses of gill gene expression revealed potential molecular mechanisms underlying salinity tolerance in this species, including the enrichment of genes involved in ion transport, maintenance of chemical homeostasis, and cell signaling in the hypersaline population. We also found differences in gene expression patterns associated with cell-cycle and protein-folding processes between the hypersaline and freshwater L. perugiae. Bidirectional freshwater-saltwater transitions have occurred repeatedly during the diversification of fishes, allowing for broad-scale examination of repeatable patterns in evolution. Therefore, we compared transcriptomic variation in L. perugiae with other teleosts that have made freshwater-saltwater transitions to test for convergence in gene expression. Among the four distantly related population pairs from high- and low-salinity environments that we included in our analysis, we found only ten shared differentially expressed genes, indicating little evidence for convergence. However, we found that differentially expressed genes shared among three or more lineages were functionally enriched for ion transport and immune functioning. Overall, our results-in conjunction with other recent studies-suggest that different genes are involved in salinity transitions across disparate lineages of teleost fishes. |
| format | Artículo científico |
| id | pubmed_39637050 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | PloS one |
| record_format | pubmed |
| spellingShingle | Gene expression signatures between Limia perugiae (Poeciliidae) populations from freshwater and hypersaline habitats, with comparisons to other teleosts. Wilson, Elizabeth J Barts, Nick Coffin, John L Johnson, James B Rodríguez Peña, Carlos M Kelley, Joanna L Tobler, Michael Greenway, Ryan Fishes Fresh Water Salinity Saline Waters Animal Distribution RNA-Seq Salt Tolerance Homeostasis Ion Transport Protein Folding Transcriptome Evolution, Molecular Gene Expression Regulation Osmoregulation Female Animals Gills Ecosystem Fish Proteins Gene expression signatures between Limia perugiae (Poeciliidae) populations from freshwater and hypersaline habitats, with comparisons to other teleosts. Wilson, Elizabeth J Barts, Nick Coffin, John L Johnson, James B Rodríguez Peña, Carlos M Kelley, Joanna L Tobler, Michael Greenway, Ryan Fishes Fresh Water Salinity Saline Waters Animal Distribution RNA-Seq Salt Tolerance Homeostasis Ion Transport Protein Folding Transcriptome Evolution, Molecular Gene Expression Regulation Osmoregulation Female Animals Gills Ecosystem Fish Proteins Salinity gradients act as strong environmental barriers that limit the distribution of aquatic organisms. Changes in gene expression associated with transitions between freshwater and saltwater environments can provide insights into organismal responses to variation in salinity. We used RNA-sequencing (RNA-seq) to investigate genome-wide variation in gene expression between a hypersaline population and a freshwater population of the livebearing fish species Limia perugiae (Poeciliidae). Our analyses of gill gene expression revealed potential molecular mechanisms underlying salinity tolerance in this species, including the enrichment of genes involved in ion transport, maintenance of chemical homeostasis, and cell signaling in the hypersaline population. We also found differences in gene expression patterns associated with cell-cycle and protein-folding processes between the hypersaline and freshwater L. perugiae. Bidirectional freshwater-saltwater transitions have occurred repeatedly during the diversification of fishes, allowing for broad-scale examination of repeatable patterns in evolution. Therefore, we compared transcriptomic variation in L. perugiae with other teleosts that have made freshwater-saltwater transitions to test for convergence in gene expression. Among the four distantly related population pairs from high- and low-salinity environments that we included in our analysis, we found only ten shared differentially expressed genes, indicating little evidence for convergence. However, we found that differentially expressed genes shared among three or more lineages were functionally enriched for ion transport and immune functioning. Overall, our results-in conjunction with other recent studies-suggest that different genes are involved in salinity transitions across disparate lineages of teleost fishes. |
| title | Gene expression signatures between Limia perugiae (Poeciliidae) populations from freshwater and hypersaline habitats, with comparisons to other teleosts. |
| topic | Fishes Fresh Water Salinity Saline Waters Animal Distribution RNA-Seq Salt Tolerance Homeostasis Ion Transport Protein Folding Transcriptome Evolution, Molecular Gene Expression Regulation Osmoregulation Female Animals Gills Ecosystem Fish Proteins |
| url | https://pubmed.ncbi.nlm.nih.gov/39637050/ |