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| Main Authors: | , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Environmental science & technology
2024
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39655750/ |
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| _version_ | 1868266270322851841 |
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| author | Li, Meizhen Kong, Liangliang Price, Neil M |
| author_facet | Li, Meizhen Kong, Liangliang Price, Neil M Li, Meizhen Kong, Liangliang Price, Neil M |
| collection | PubMed - marine biology |
| contents | The Effect of Copper Limitation on the Bioavailability of Cu-Organic Complexes. Li, Meizhen Kong, Liangliang Price, Neil M Copper Biological Availability Phytoplankton Diatoms Photosynthesis We examined the bioavailability of synthetic organic Cu complexes (Cu-L) and inorganic Cu species (Cu') to growing under Cu-limiting and Cu-inhibiting conditions. Copper bioavailability depended on the phytoplankton Cu nutritional state and whether Cu' diffusion to the cell surface was sufficient to meet the cellular demands for growth. Under Cu-limiting conditions when [Cu'] was less than the diffusion concentration threshold (DCT: 10 M), growth rate was a hyperbolic function of [Cu-L]. Increasing [Cu'] above the DCT caused growth rate to increase proportionally, but growth rate also increased with increasing [Cu-L] so that both Cu' and Cu-L were bioavailable. Short-term photosynthesis assays conducted under Cu-limiting conditions showed a similar response to Cu speciation. In contrast, the growth rate of Cu-inhibited cells at high [Cu'] varied inversely with [Cu] and was independent of [Cu-L], as previously reported. The change in Cu-L bioavailability correlated with expression levels of genes encoding the reduction-dependent, high-affinity Cu uptake pathway, which was regulated by [Cu']. Our analysis shows that at [Cu'] typical of the open sea, -like phytoplankton are diffusion limited and must rely on Cu-organic complexes to fulfill their Cu requirements for growth. |
| format | Artículo científico |
| id | pubmed_39655750 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Environmental science & technology |
| record_format | pubmed |
| spellingShingle | The Effect of Copper Limitation on the Bioavailability of Cu-Organic Complexes. Li, Meizhen Kong, Liangliang Price, Neil M Copper Biological Availability Phytoplankton Diatoms Photosynthesis The Effect of Copper Limitation on the Bioavailability of Cu-Organic Complexes. Li, Meizhen Kong, Liangliang Price, Neil M Copper Biological Availability Phytoplankton Diatoms Photosynthesis We examined the bioavailability of synthetic organic Cu complexes (Cu-L) and inorganic Cu species (Cu') to growing under Cu-limiting and Cu-inhibiting conditions. Copper bioavailability depended on the phytoplankton Cu nutritional state and whether Cu' diffusion to the cell surface was sufficient to meet the cellular demands for growth. Under Cu-limiting conditions when [Cu'] was less than the diffusion concentration threshold (DCT: 10 M), growth rate was a hyperbolic function of [Cu-L]. Increasing [Cu'] above the DCT caused growth rate to increase proportionally, but growth rate also increased with increasing [Cu-L] so that both Cu' and Cu-L were bioavailable. Short-term photosynthesis assays conducted under Cu-limiting conditions showed a similar response to Cu speciation. In contrast, the growth rate of Cu-inhibited cells at high [Cu'] varied inversely with [Cu] and was independent of [Cu-L], as previously reported. The change in Cu-L bioavailability correlated with expression levels of genes encoding the reduction-dependent, high-affinity Cu uptake pathway, which was regulated by [Cu']. Our analysis shows that at [Cu'] typical of the open sea, -like phytoplankton are diffusion limited and must rely on Cu-organic complexes to fulfill their Cu requirements for growth. |
| title | The Effect of Copper Limitation on the Bioavailability of Cu-Organic Complexes. |
| topic | Copper Biological Availability Phytoplankton Diatoms Photosynthesis |
| url | https://pubmed.ncbi.nlm.nih.gov/39655750/ |