A Crystal Structure of Pro c 2 Provides Insights into Cross-Reactivity of Aquatic Allergens from the Phosphagen Kinase Family.
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| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Journal of agricultural and food chemistry
2024
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| Acceso en línea: | |
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| _version_ | 1868266270309220353 |
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| author | Yang, Yang He, Xin-Rong Huan, Fei Bai, Tian-Liang Zhang, Qi-Hui Li, Fa-Jie Chen, Gui-Xia Zheng, Pei-Yi Xu, Li-Mei Liu, Guang-Ming |
| author_facet | Yang, Yang He, Xin-Rong Huan, Fei Bai, Tian-Liang Zhang, Qi-Hui Li, Fa-Jie Chen, Gui-Xia Zheng, Pei-Yi Xu, Li-Mei Liu, Guang-Ming Yang, Yang He, Xin-Rong Huan, Fei Bai, Tian-Liang Zhang, Qi-Hui Li, Fa-Jie Chen, Gui-Xia Zheng, Pei-Yi Xu, Li-Mei Liu, Guang-Ming |
| collection | PubMed - marine biology |
| contents | A Crystal Structure of Pro c 2 Provides Insights into Cross-Reactivity of Aquatic Allergens from the Phosphagen Kinase Family. Yang, Yang He, Xin-Rong Huan, Fei Bai, Tian-Liang Zhang, Qi-Hui Li, Fa-Jie Chen, Gui-Xia Zheng, Pei-Yi Xu, Li-Mei Liu, Guang-Ming Animals Allergens Cross Reactions Arginine Kinase Humans Fish Proteins Immunoglobulin E Amino Acid Sequence Creatine Kinase Shellfish Immunoglobulin G Arginine kinase (AK) from the phosphagen kinase family is a cross-reactive shellfish allergen. Structurally related cross-reactive allergens are involved in the pathogenesis of allergic symptoms. This study aimed to unravel the cross-reactivity of AK from a structural perspective. The crystal structure of AK (Pro c 2) was resolved at 1.57 Å resolution, which showed a well-conserved structure not only to shellfish AKs but also to fish creatine kinase (CK), another allergen from the phosphagen kinase family. In Western blot, the CK corresponding protein in fish muscles was found to be reactive with AK-specific immunoglobulin (Ig) G. Recombinant Pro c 2 (rPro c 2) and CKs from (rCK-) and (rCK-) were then produced, and the IgE reactivity of rCK- and rCK-, as well as their IgG/IgE cross-reactivity with rPro c 2, was confirmed by immunological assays. This study demonstrated the cross-reactivity among aquatic allergens from the phosphagen kinase family due to their structural similarity. |
| format | Artículo científico |
| id | pubmed_39661082 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Journal of agricultural and food chemistry |
| record_format | pubmed |
| spellingShingle | A Crystal Structure of Pro c 2 Provides Insights into Cross-Reactivity of Aquatic Allergens from the Phosphagen Kinase Family. Yang, Yang He, Xin-Rong Huan, Fei Bai, Tian-Liang Zhang, Qi-Hui Li, Fa-Jie Chen, Gui-Xia Zheng, Pei-Yi Xu, Li-Mei Liu, Guang-Ming Animals Allergens Cross Reactions Arginine Kinase Humans Fish Proteins Immunoglobulin E Amino Acid Sequence Creatine Kinase Shellfish Immunoglobulin G A Crystal Structure of Pro c 2 Provides Insights into Cross-Reactivity of Aquatic Allergens from the Phosphagen Kinase Family. Yang, Yang He, Xin-Rong Huan, Fei Bai, Tian-Liang Zhang, Qi-Hui Li, Fa-Jie Chen, Gui-Xia Zheng, Pei-Yi Xu, Li-Mei Liu, Guang-Ming Animals Allergens Cross Reactions Arginine Kinase Humans Fish Proteins Immunoglobulin E Amino Acid Sequence Creatine Kinase Shellfish Immunoglobulin G Arginine kinase (AK) from the phosphagen kinase family is a cross-reactive shellfish allergen. Structurally related cross-reactive allergens are involved in the pathogenesis of allergic symptoms. This study aimed to unravel the cross-reactivity of AK from a structural perspective. The crystal structure of AK (Pro c 2) was resolved at 1.57 Å resolution, which showed a well-conserved structure not only to shellfish AKs but also to fish creatine kinase (CK), another allergen from the phosphagen kinase family. In Western blot, the CK corresponding protein in fish muscles was found to be reactive with AK-specific immunoglobulin (Ig) G. Recombinant Pro c 2 (rPro c 2) and CKs from (rCK-) and (rCK-) were then produced, and the IgE reactivity of rCK- and rCK-, as well as their IgG/IgE cross-reactivity with rPro c 2, was confirmed by immunological assays. This study demonstrated the cross-reactivity among aquatic allergens from the phosphagen kinase family due to their structural similarity. |
| title | A Crystal Structure of Pro c 2 Provides Insights into Cross-Reactivity of Aquatic Allergens from the Phosphagen Kinase Family. |
| topic | Animals Allergens Cross Reactions Arginine Kinase Humans Fish Proteins Immunoglobulin E Amino Acid Sequence Creatine Kinase Shellfish Immunoglobulin G |
| url | https://pubmed.ncbi.nlm.nih.gov/39661082/ |