Multimodule Synthetic Redesign of Intracellular Metabolisms for the High-Titer de Novo Production of Sakuranetin in .
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| Autori principali: | , , , , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
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Journal of agricultural and food chemistry
2024
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| _version_ | 1868266270308171777 |
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| author | Ge, Jianyue Lu, Xueyao Liu, Cancan Liu, Wei Wu, Na Feng, Bingxuan Sun, Xiaoman Gu, Yang |
| author_facet | Ge, Jianyue Lu, Xueyao Liu, Cancan Liu, Wei Wu, Na Feng, Bingxuan Sun, Xiaoman Gu, Yang Ge, Jianyue Lu, Xueyao Liu, Cancan Liu, Wei Wu, Na Feng, Bingxuan Sun, Xiaoman Gu, Yang |
| collection | PubMed - marine biology |
| contents | Multimodule Synthetic Redesign of Intracellular Metabolisms for the High-Titer de Novo Production of Sakuranetin in . Ge, Jianyue Lu, Xueyao Liu, Cancan Liu, Wei Wu, Na Feng, Bingxuan Sun, Xiaoman Gu, Yang Yarrowia Metabolic Engineering Glucose Flavonoids Biosynthetic Pathways Phytoalexins Sakuranetin, a flavonoid phytoalexin, has demonstrated neuroprotective properties and exhibits tyrosinase inhibitory activities, making it highly valuable in the cosmetics and pharmaceutical industries. In this study, we engineered a strain for the high-titer de novo production of sakuranetin using glucose as a substrate. To effectively enhance sakuranetin production, we implemented a multimodule engineering strategy that included optimizing the sakuranetin synthesis pathway, designing a regeneration system for the methyl donor S-adenosyl methionine, increasing the malonyl-CoA precursor supplement, and constructing the feedback inhibition-relieved shikimate pathway. Moreover, a transcriptomic analysis was conducted to identify potential targets for further improving sakuranetin synthesis. As a result, the titer of de novo synthesized sakuranetin reached 344.0 mg/L from glucose in a 5 L bioreactor. These achievements hold significant promise for the sustainable and large-scale production of sakuranetin through industrial biomanufacturing. |
| format | Artículo científico |
| id | pubmed_39661494 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Journal of agricultural and food chemistry |
| record_format | pubmed |
| spellingShingle | Multimodule Synthetic Redesign of Intracellular Metabolisms for the High-Titer de Novo Production of Sakuranetin in . Ge, Jianyue Lu, Xueyao Liu, Cancan Liu, Wei Wu, Na Feng, Bingxuan Sun, Xiaoman Gu, Yang Yarrowia Metabolic Engineering Glucose Flavonoids Biosynthetic Pathways Phytoalexins Multimodule Synthetic Redesign of Intracellular Metabolisms for the High-Titer de Novo Production of Sakuranetin in . Ge, Jianyue Lu, Xueyao Liu, Cancan Liu, Wei Wu, Na Feng, Bingxuan Sun, Xiaoman Gu, Yang Yarrowia Metabolic Engineering Glucose Flavonoids Biosynthetic Pathways Phytoalexins Sakuranetin, a flavonoid phytoalexin, has demonstrated neuroprotective properties and exhibits tyrosinase inhibitory activities, making it highly valuable in the cosmetics and pharmaceutical industries. In this study, we engineered a strain for the high-titer de novo production of sakuranetin using glucose as a substrate. To effectively enhance sakuranetin production, we implemented a multimodule engineering strategy that included optimizing the sakuranetin synthesis pathway, designing a regeneration system for the methyl donor S-adenosyl methionine, increasing the malonyl-CoA precursor supplement, and constructing the feedback inhibition-relieved shikimate pathway. Moreover, a transcriptomic analysis was conducted to identify potential targets for further improving sakuranetin synthesis. As a result, the titer of de novo synthesized sakuranetin reached 344.0 mg/L from glucose in a 5 L bioreactor. These achievements hold significant promise for the sustainable and large-scale production of sakuranetin through industrial biomanufacturing. |
| title | Multimodule Synthetic Redesign of Intracellular Metabolisms for the High-Titer de Novo Production of Sakuranetin in . |
| topic | Yarrowia Metabolic Engineering Glucose Flavonoids Biosynthetic Pathways Phytoalexins |
| url | https://pubmed.ncbi.nlm.nih.gov/39661494/ |