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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of agricultural and food chemistry
2024
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39661494/ |
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Table of Contents:
- Multimodule Synthetic Redesign of Intracellular Metabolisms for the High-Titer de Novo Production of Sakuranetin in . Ge, Jianyue Lu, Xueyao Liu, Cancan Liu, Wei Wu, Na Feng, Bingxuan Sun, Xiaoman Gu, Yang Yarrowia Metabolic Engineering Glucose Flavonoids Biosynthetic Pathways Phytoalexins Sakuranetin, a flavonoid phytoalexin, has demonstrated neuroprotective properties and exhibits tyrosinase inhibitory activities, making it highly valuable in the cosmetics and pharmaceutical industries. In this study, we engineered a strain for the high-titer de novo production of sakuranetin using glucose as a substrate. To effectively enhance sakuranetin production, we implemented a multimodule engineering strategy that included optimizing the sakuranetin synthesis pathway, designing a regeneration system for the methyl donor S-adenosyl methionine, increasing the malonyl-CoA precursor supplement, and constructing the feedback inhibition-relieved shikimate pathway. Moreover, a transcriptomic analysis was conducted to identify potential targets for further improving sakuranetin synthesis. As a result, the titer of de novo synthesized sakuranetin reached 344.0 mg/L from glucose in a 5 L bioreactor. These achievements hold significant promise for the sustainable and large-scale production of sakuranetin through industrial biomanufacturing.