Neuroendocrine regulatory network underlying physiological shifts between reproduction and aestivation of sea cucumber Apostichopus japonicus: Insights from transcriptome analysis.
Fuente:
PubMed
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Comparative biochemistry and physiology. Part D, Genomics & proteomics
2025
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266268231991297 |
|---|---|
| author | Ge, Lifei Wang, Jixiu Jiang, Zhijing Ye, Zhiqing Wei, Ying Wang, Yibo Liu, Yuting Wu, Chenqian Xu, Xiuwen Yang, Jingwen Sun, Lina Wang, Tianming |
| author_facet | Ge, Lifei Wang, Jixiu Jiang, Zhijing Ye, Zhiqing Wei, Ying Wang, Yibo Liu, Yuting Wu, Chenqian Xu, Xiuwen Yang, Jingwen Sun, Lina Wang, Tianming Ge, Lifei Wang, Jixiu Jiang, Zhijing Ye, Zhiqing Wei, Ying Wang, Yibo Liu, Yuting Wu, Chenqian Xu, Xiuwen Yang, Jingwen Sun, Lina Wang, Tianming |
| collection | PubMed - marine biology |
| contents | Neuroendocrine regulatory network underlying physiological shifts between reproduction and aestivation of sea cucumber Apostichopus japonicus: Insights from transcriptome analysis. Ge, Lifei Wang, Jixiu Jiang, Zhijing Ye, Zhiqing Wei, Ying Wang, Yibo Liu, Yuting Wu, Chenqian Xu, Xiuwen Yang, Jingwen Sun, Lina Wang, Tianming Animals Reproduction Stichopus Transcriptome Gene Expression Profiling Estivation Neurosecretory Systems Sea Cucumbers Gene Regulatory Networks The sea cucumber Apostichopus japonicus is an economically significant marine species in China, with numerous studies focusing on its diverse physiological processes, including seasonal reproduction and aestivation. The neuroendocrine system plays a critical role in regulating these physiological transitions. In this study, we utilized transcriptomic techniques and bioinformatics tools to identify key functional genes in the nerve ring of A. japonicus during four distinct physiological phases: pre-reproduction, post-reproduction, aestivation, and recovery from aestivation. Totally 33 neuropeptide precursors and 521 G protein-coupled receptors (GPCRs) were identified. Highlighted key genes, such as AjCRZP, AjPDFP1b, AjSSP1, AjSSP2, and AjSSTR4, were proposed to contribute to transitions of reproduction to aestivation. The temporal trends and functions of differentially expressed genes (DEGs) were validated through qRT-PCR assays. Additionally, we constructed a preliminary neuroendocrine regulatory network, with AjGALR2 and AjCHRM5 identified as central hub genes. These findings offer valuable insights into the neuroendocrine regulation of reproduction and aestivation in A. japonicus, providing a foundation for further mechanistic studies and enhancing our understanding of sea cucumber biology. |
| format | Artículo científico |
| id | pubmed_39675199 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Comparative biochemistry and physiology. Part D, Genomics & proteomics |
| record_format | pubmed |
| spellingShingle | Neuroendocrine regulatory network underlying physiological shifts between reproduction and aestivation of sea cucumber Apostichopus japonicus: Insights from transcriptome analysis. Ge, Lifei Wang, Jixiu Jiang, Zhijing Ye, Zhiqing Wei, Ying Wang, Yibo Liu, Yuting Wu, Chenqian Xu, Xiuwen Yang, Jingwen Sun, Lina Wang, Tianming Animals Reproduction Stichopus Transcriptome Gene Expression Profiling Estivation Neurosecretory Systems Sea Cucumbers Gene Regulatory Networks Neuroendocrine regulatory network underlying physiological shifts between reproduction and aestivation of sea cucumber Apostichopus japonicus: Insights from transcriptome analysis. Ge, Lifei Wang, Jixiu Jiang, Zhijing Ye, Zhiqing Wei, Ying Wang, Yibo Liu, Yuting Wu, Chenqian Xu, Xiuwen Yang, Jingwen Sun, Lina Wang, Tianming Animals Reproduction Stichopus Transcriptome Gene Expression Profiling Estivation Neurosecretory Systems Sea Cucumbers Gene Regulatory Networks The sea cucumber Apostichopus japonicus is an economically significant marine species in China, with numerous studies focusing on its diverse physiological processes, including seasonal reproduction and aestivation. The neuroendocrine system plays a critical role in regulating these physiological transitions. In this study, we utilized transcriptomic techniques and bioinformatics tools to identify key functional genes in the nerve ring of A. japonicus during four distinct physiological phases: pre-reproduction, post-reproduction, aestivation, and recovery from aestivation. Totally 33 neuropeptide precursors and 521 G protein-coupled receptors (GPCRs) were identified. Highlighted key genes, such as AjCRZP, AjPDFP1b, AjSSP1, AjSSP2, and AjSSTR4, were proposed to contribute to transitions of reproduction to aestivation. The temporal trends and functions of differentially expressed genes (DEGs) were validated through qRT-PCR assays. Additionally, we constructed a preliminary neuroendocrine regulatory network, with AjGALR2 and AjCHRM5 identified as central hub genes. These findings offer valuable insights into the neuroendocrine regulation of reproduction and aestivation in A. japonicus, providing a foundation for further mechanistic studies and enhancing our understanding of sea cucumber biology. |
| title | Neuroendocrine regulatory network underlying physiological shifts between reproduction and aestivation of sea cucumber Apostichopus japonicus: Insights from transcriptome analysis. |
| topic | Animals Reproduction Stichopus Transcriptome Gene Expression Profiling Estivation Neurosecretory Systems Sea Cucumbers Gene Regulatory Networks |
| url | https://pubmed.ncbi.nlm.nih.gov/39675199/ |