Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Glycoconjugate journal
2024
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39680336/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266268221505538 |
|---|---|
| author | Yang, Jiyuan Datta, Payel Xia, Ke Pomin, Vitor H Wang, Chunyu Qiao, Mingqiang Linhardt, Robert J Dordick, Jonathan S Zhang, Fuming |
| author_facet | Yang, Jiyuan Datta, Payel Xia, Ke Pomin, Vitor H Wang, Chunyu Qiao, Mingqiang Linhardt, Robert J Dordick, Jonathan S Zhang, Fuming Yang, Jiyuan Datta, Payel Xia, Ke Pomin, Vitor H Wang, Chunyu Qiao, Mingqiang Linhardt, Robert J Dordick, Jonathan S Zhang, Fuming |
| collection | PubMed - marine biology |
| contents | Inhibition of sulfated glycans on the binding of dengue virus envelope protein to heparin. Yang, Jiyuan Datta, Payel Xia, Ke Pomin, Vitor H Wang, Chunyu Qiao, Mingqiang Linhardt, Robert J Dordick, Jonathan S Zhang, Fuming Heparin Viral Envelope Proteins Dengue Virus Protein Binding Polysaccharides Humans Heparan Sulfate Dengue viruses (DENV) are transmitted to humans through mosquito bites and infect millions globally. DENV uses heparan sulfate (HS) for attachment and cell entry by binding the envelope protein to highly sulfated HS on target cells. Therefore, inhibiting the binding between DENV and HS could be a promising strategy for preventing DENV infection. In the current study, the interactions between DENV envelope protein (from Type 2 DENV) and heparin (a surrogate for HS) were analyzed using competition solution SPR. Results demonstrate that heparin binds to DENV envelope protein with high affinity (K = 8.83 nM). Competitive Solution SPR assays using surface-immobilized heparin and a series of naturally-sourced and semi-synthetic sulfated glycans demonstrated significant inhibitory activity against the binding of DENV envelope proteins to heparin. This study of molecular interactions could provide insights into the development of therapeutics for DENV infection. |
| format | Artículo científico |
| id | pubmed_39680336 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Glycoconjugate journal |
| record_format | pubmed |
| spellingShingle | Inhibition of sulfated glycans on the binding of dengue virus envelope protein to heparin. Yang, Jiyuan Datta, Payel Xia, Ke Pomin, Vitor H Wang, Chunyu Qiao, Mingqiang Linhardt, Robert J Dordick, Jonathan S Zhang, Fuming Heparin Viral Envelope Proteins Dengue Virus Protein Binding Polysaccharides Humans Heparan Sulfate Inhibition of sulfated glycans on the binding of dengue virus envelope protein to heparin. Yang, Jiyuan Datta, Payel Xia, Ke Pomin, Vitor H Wang, Chunyu Qiao, Mingqiang Linhardt, Robert J Dordick, Jonathan S Zhang, Fuming Heparin Viral Envelope Proteins Dengue Virus Protein Binding Polysaccharides Humans Heparan Sulfate Dengue viruses (DENV) are transmitted to humans through mosquito bites and infect millions globally. DENV uses heparan sulfate (HS) for attachment and cell entry by binding the envelope protein to highly sulfated HS on target cells. Therefore, inhibiting the binding between DENV and HS could be a promising strategy for preventing DENV infection. In the current study, the interactions between DENV envelope protein (from Type 2 DENV) and heparin (a surrogate for HS) were analyzed using competition solution SPR. Results demonstrate that heparin binds to DENV envelope protein with high affinity (K = 8.83 nM). Competitive Solution SPR assays using surface-immobilized heparin and a series of naturally-sourced and semi-synthetic sulfated glycans demonstrated significant inhibitory activity against the binding of DENV envelope proteins to heparin. This study of molecular interactions could provide insights into the development of therapeutics for DENV infection. |
| title | Inhibition of sulfated glycans on the binding of dengue virus envelope protein to heparin. |
| topic | Heparin Viral Envelope Proteins Dengue Virus Protein Binding Polysaccharides Humans Heparan Sulfate |
| url | https://pubmed.ncbi.nlm.nih.gov/39680336/ |