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| Format: | Artículo científico |
| Language: | en |
| Published: |
Environmental microbiology
2024
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39702997/ |
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| author | Kong, Yachao Riebe, Jan Feßner, Malte Schaller, Torsten Wölper, Christoph Stappert, Florian Meckelmann, Sven W Krajnc, Matthias Weyrauch, Philip Schmitz, Oliver J Merten, Christian Niemeyer, Jochen Hu, Xiaoke Meckenstock, Rainer U |
| author_facet | Kong, Yachao Riebe, Jan Feßner, Malte Schaller, Torsten Wölper, Christoph Stappert, Florian Meckelmann, Sven W Krajnc, Matthias Weyrauch, Philip Schmitz, Oliver J Merten, Christian Niemeyer, Jochen Hu, Xiaoke Meckenstock, Rainer U Kong, Yachao Riebe, Jan Feßner, Malte Schaller, Torsten Wölper, Christoph Stappert, Florian Meckelmann, Sven W Krajnc, Matthias Weyrauch, Philip Schmitz, Oliver J Merten, Christian Niemeyer, Jochen Hu, Xiaoke Meckenstock, Rainer U |
| collection | PubMed - marine biology |
| contents | A CoA-Transferase and Acyl-CoA Dehydrogenase Convert 2-(Carboxymethyl)cyclohexane-1-Carboxyl-CoA During Anaerobic Naphthalene Degradation. Kong, Yachao Riebe, Jan Feßner, Malte Schaller, Torsten Wölper, Christoph Stappert, Florian Meckelmann, Sven W Krajnc, Matthias Weyrauch, Philip Schmitz, Oliver J Merten, Christian Niemeyer, Jochen Hu, Xiaoke Meckenstock, Rainer U Naphthalenes Anaerobiosis Biodegradation, Environmental Coenzyme A-Transferases Acyl-CoA Dehydrogenase Operon Bacterial Proteins The CoA thioester of 2-(carboxymethyl)cyclohexane-1-carboxylic acid has been identified as a metabolite in anaerobic naphthalene degradation by the sulfate-reducing culture N47. This study identified and characterised two acyl-CoA dehydrogenases (ThnO/ThnT) and an intramolecular CoA-transferase (ThnP) encoded within the substrate-induced thn operon, which contains genes for anaerobic degradation of naphthalene. ThnP is a CoA transferase belonging to the family I (Cat 1 subgroup) that catalyses the intramolecular CoA transfer from the carboxyl group of 2-(carboxymethyl)cyclohexane-1-carboxyl-CoA to its carboxymethyl moiety, forming 2-carboxycyclohexylacetyl-CoA. Neither acetyl-CoA nor succinyl-CoA functions as an exogenous CoA donor for this reaction. The flavin-dependent homotetrameric dehydrogenase ThnO is specific for (1R,2R)-2-carboxycyclohexylacetyl-CoA with an apparent K value of 61.5 μM, whereas ThnT is a promiscuous enzyme catalysing the same reaction at lower rates. Identifying these three enzymes confirmed the involvement of the thn gene cluster in the anaerobic naphthalene degradation pathway. This study establishes a modified metabolic pathway for anaerobic naphthalene degradation upstream of 2-(carboxymethyl)cyclohexane-1-carboxyl-CoA and provides further insight into the subsequent second-ring cleavage reaction. |
| format | Artículo científico |
| id | pubmed_39702997 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Environmental microbiology |
| record_format | pubmed |
| spellingShingle | A CoA-Transferase and Acyl-CoA Dehydrogenase Convert 2-(Carboxymethyl)cyclohexane-1-Carboxyl-CoA During Anaerobic Naphthalene Degradation. Kong, Yachao Riebe, Jan Feßner, Malte Schaller, Torsten Wölper, Christoph Stappert, Florian Meckelmann, Sven W Krajnc, Matthias Weyrauch, Philip Schmitz, Oliver J Merten, Christian Niemeyer, Jochen Hu, Xiaoke Meckenstock, Rainer U Naphthalenes Anaerobiosis Biodegradation, Environmental Coenzyme A-Transferases Acyl-CoA Dehydrogenase Operon Bacterial Proteins A CoA-Transferase and Acyl-CoA Dehydrogenase Convert 2-(Carboxymethyl)cyclohexane-1-Carboxyl-CoA During Anaerobic Naphthalene Degradation. Kong, Yachao Riebe, Jan Feßner, Malte Schaller, Torsten Wölper, Christoph Stappert, Florian Meckelmann, Sven W Krajnc, Matthias Weyrauch, Philip Schmitz, Oliver J Merten, Christian Niemeyer, Jochen Hu, Xiaoke Meckenstock, Rainer U Naphthalenes Anaerobiosis Biodegradation, Environmental Coenzyme A-Transferases Acyl-CoA Dehydrogenase Operon Bacterial Proteins The CoA thioester of 2-(carboxymethyl)cyclohexane-1-carboxylic acid has been identified as a metabolite in anaerobic naphthalene degradation by the sulfate-reducing culture N47. This study identified and characterised two acyl-CoA dehydrogenases (ThnO/ThnT) and an intramolecular CoA-transferase (ThnP) encoded within the substrate-induced thn operon, which contains genes for anaerobic degradation of naphthalene. ThnP is a CoA transferase belonging to the family I (Cat 1 subgroup) that catalyses the intramolecular CoA transfer from the carboxyl group of 2-(carboxymethyl)cyclohexane-1-carboxyl-CoA to its carboxymethyl moiety, forming 2-carboxycyclohexylacetyl-CoA. Neither acetyl-CoA nor succinyl-CoA functions as an exogenous CoA donor for this reaction. The flavin-dependent homotetrameric dehydrogenase ThnO is specific for (1R,2R)-2-carboxycyclohexylacetyl-CoA with an apparent K value of 61.5 μM, whereas ThnT is a promiscuous enzyme catalysing the same reaction at lower rates. Identifying these three enzymes confirmed the involvement of the thn gene cluster in the anaerobic naphthalene degradation pathway. This study establishes a modified metabolic pathway for anaerobic naphthalene degradation upstream of 2-(carboxymethyl)cyclohexane-1-carboxyl-CoA and provides further insight into the subsequent second-ring cleavage reaction. |
| title | A CoA-Transferase and Acyl-CoA Dehydrogenase Convert 2-(Carboxymethyl)cyclohexane-1-Carboxyl-CoA During Anaerobic Naphthalene Degradation. |
| topic | Naphthalenes Anaerobiosis Biodegradation, Environmental Coenzyme A-Transferases Acyl-CoA Dehydrogenase Operon Bacterial Proteins |
| url | https://pubmed.ncbi.nlm.nih.gov/39702997/ |