Shotgun Proteomics Protocol for Insects.
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| Format: | Artículo científico |
| Sprache: | en |
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Methods in molecular biology (Clifton, N.J.)
2025
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| author | Varunjikar, Madhushri S Belghit, Ikram Oveland, Eystein Palmblad, Magnus Rasinger, Josef D |
| author_facet | Varunjikar, Madhushri S Belghit, Ikram Oveland, Eystein Palmblad, Magnus Rasinger, Josef D Varunjikar, Madhushri S Belghit, Ikram Oveland, Eystein Palmblad, Magnus Rasinger, Josef D |
| collection | PubMed - marine biology |
| contents | Shotgun Proteomics Protocol for Insects. Varunjikar, Madhushri S Belghit, Ikram Oveland, Eystein Palmblad, Magnus Rasinger, Josef D Animals Proteomics Insecta Insect Proteins Software Computational Biology Chromatography, High Pressure Liquid Tandem Mass Spectrometry Proteome Peptides Trypsin Shotgun proteomics can be applied to identify and study insect species in diverse research areas such as agriculture, forensics, biodiversity conservation, and food safety. In this chapter, we have provided a detailed protocol for shotgun proteomics analytical methods involving enzymatic digestion of insect proteins using trypsin, separation using high-performance liquid chromatography, and detection of separated peptides using high-resolution mass spectrometry. The protocol also covers the utilization of bioinformatics software for protein identification and spectral library building, proposing both proteomic database-dependent and independent methods. This chapter provides a valuable foundation for applying insect proteomics by discussing a step-by-step protocol. |
| format | Artículo científico |
| id | pubmed_39715999 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Methods in molecular biology (Clifton, N.J.) |
| record_format | pubmed |
| spellingShingle | Shotgun Proteomics Protocol for Insects. Varunjikar, Madhushri S Belghit, Ikram Oveland, Eystein Palmblad, Magnus Rasinger, Josef D Animals Proteomics Insecta Insect Proteins Software Computational Biology Chromatography, High Pressure Liquid Tandem Mass Spectrometry Proteome Peptides Trypsin Shotgun Proteomics Protocol for Insects. Varunjikar, Madhushri S Belghit, Ikram Oveland, Eystein Palmblad, Magnus Rasinger, Josef D Animals Proteomics Insecta Insect Proteins Software Computational Biology Chromatography, High Pressure Liquid Tandem Mass Spectrometry Proteome Peptides Trypsin Shotgun proteomics can be applied to identify and study insect species in diverse research areas such as agriculture, forensics, biodiversity conservation, and food safety. In this chapter, we have provided a detailed protocol for shotgun proteomics analytical methods involving enzymatic digestion of insect proteins using trypsin, separation using high-performance liquid chromatography, and detection of separated peptides using high-resolution mass spectrometry. The protocol also covers the utilization of bioinformatics software for protein identification and spectral library building, proposing both proteomic database-dependent and independent methods. This chapter provides a valuable foundation for applying insect proteomics by discussing a step-by-step protocol. |
| title | Shotgun Proteomics Protocol for Insects. |
| topic | Animals Proteomics Insecta Insect Proteins Software Computational Biology Chromatography, High Pressure Liquid Tandem Mass Spectrometry Proteome Peptides Trypsin |
| url | https://pubmed.ncbi.nlm.nih.gov/39715999/ |