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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Marine drugs
2024
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39728138/ |
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Table of Contents:
- Chemical Composition, Free Radicals and Pathogenic Microbes in the Extract Derived from : In Silico and In Vitro Approaches. Oumassi, Fouad Chebbac, Khalid Ben Ali, Naouar Kaabi, Soundouss El Ansari, Zineb Nejjar Metouekel, Amira El Barnossi, Azeddin El Moussaoui, Abdelfattah Chebaibi, Mohamed Bounab, Loubna Mssillou, Ibrahim Shahat, Abdelaaty Abdelaziz El Bouzdoudi, Brahim L'bachir El Kbiach, Mohammed Antioxidants Computer Simulation Anti-Bacterial Agents Plant Extracts Microbial Sensitivity Tests Free Radicals Seaweed Chromatography, High Pressure Liquid Antifungal Agents Anti-Infective Agents Escherichia coli Marine algae are renowned for their health benefits due to the presence of functional bioactive compounds. In this context, this study aims to valorize the extract of a seaweed, (), through phytochemical characterization using liquid chromatography-mass spectrometry (HPLC-MS), as well as in vitro and in silico evaluation of its biological activities (antioxidant and antimicrobial). Phytochemical characterization revealed that the ethanolic extract of (DdEx) is rich in phenolic compounds, with a total of 22 phycocompounds identified. Antioxidant activity, measured by various methods, showed an IC of 120 µg/mL for the DPPH assay, an EC of 120.53 µg/mL for the FRAP assay, and a total antioxidant power of 685.26 µg AAE/mg according to the phosphomolybdate (TAC) method. Evaluation of antibacterial activity showed a zone of inhibition diameter ranging from 11.93 to 22.58 mm, with the largest zone observed for the () strain. For antifungal activity, inhibition zone diameters ranged from 22.38 to 23.52 mm, with the largest recorded for the () strain. The in silico study identified tetragalloyl-glucose, apigenin-7-O-glucoside, and pentagalloyl-glucose as the most active compounds against NADPH oxidase, with docking scores of -7.723, -7.424, and -6.402 kcal/mol, respectively. Regarding antibacterial activity, apigenin-7-O-glucoside, pelargonidin-3-O-glucoside, and secoisolariciresinol demonstrated high affinity for beta-ketoacyl-[acyl carrier protein] synthase, with docking scores of -7.276, -6.811, and -6.594 kcal/mol, respectively. These in vitro and in silico evaluations showed that extract possesses antioxidant and antimicrobial properties, due to its richness in bioactive compounds identified by HPLC.