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| Format: | Artículo científico |
| Sprache: | en |
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Journal of photochemistry and photobiology. B, Biology
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/39732112/ |
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Inhaltsangabe:
- Novel peptide inhibitor of matrix Metalloproteinases-1 from pufferfish skin collagen hydrolysates and its potential Photoprotective activity via the MAPK/AP-1 signaling pathway. Chen, Bei Cai, Shuilin Cui, Lulu Yu, Ting Qiao, Kun Su, Yongchang Xu, Min Tang, Haiyan Liu, Shuji Yang, Ming Liu, Zhiyu Animals Skin Collagen Ultraviolet Rays Mice Transcription Factor AP-1 Molecular Docking Simulation Reactive Oxygen Species Matrix Metalloproteinase Inhibitors Peptides Takifugu Cell Line Matrix Metalloproteinase 1 Signal Transduction MAP Kinase Signaling System Amino Acid Sequence Protein Hydrolysates Fish Proteins Cell Survival Takifugu bimaculatus, a pufferfish species farmed in Fujian Province, is known for its non-toxic flesh and collagen-rich skin. We identified a novel collagen-derived matrix metalloproteinase 1 (MMP-1) inhibitory peptide, from T. bimaculatus skin with potent anti-photoaging properties. Using multistage membrane and gel filtration chromatography, we purified low-molecular-weight collagen peptides from T. bimaculatus skin (TBSCH-L). Nano-HPLC-MS/MS and virtual molecular docking screening were employed to identify peptides targeting MMP-1. Four anti-photoaging peptide sequences, GDRGFPGE, GPAGPRGA, FPGGPGAK, and RGFPGGDGAA, were identified by assessing the viability of UVB-induced L929 cells. GPAGPRGA (GP8) exhibited the highest MMP-1 inhibitory activity and cellular photoprotection. Surface plasmon resonance confirmed high-affinity binding between MMP-1 and GP8. GP8 significantly reduced intracellular reactive oxygen species (ROS) levels and enhanced superoxide dismutase activity at concentrations of 100-200 μM in UVB-exposed L929 cells. At 200 μM, GP8 significantly decreased malondialdehyde content. GP8 also accelerated migration of L929 cells, demonstrating its wound-healing potential, markedly reduced intracellular β-galactosidase levels, and downregulated phosphorylation levels of extracellular signal-regulated kinases, c-Jun N-terminal kinases, p38 proteins, and c-Jun protein expression within the MAPK/AP-1 signaling pathway, thereby lowering MMP expression in L929 cells. Exposure of zebrafish to 25-100 μM GP8 effectively mitigated UVB-induced damage, restoring up to 31.2 % of caudal fin integrity, while significantly reducing ROS levels, lipid peroxidation, and cellular apoptosis. GP8, a novel marine-derived anti-photoaging peptide, holds promise for applications in cosmetic and functional food sectors.