A vaccine combining ORF132 and ORF25 expressed by Saccharomyces cerevisiae induces protective immunity in Carassius auratus gibelio against CyHV-2.
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Fish & shellfish immunology
2025
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| author | Liang, Jiahui Yang, Maoxia Li, Xinmei Zhou, Qianjin Yang, Guanjun Lu, Jianfei Chen, Jiong |
| author_facet | Liang, Jiahui Yang, Maoxia Li, Xinmei Zhou, Qianjin Yang, Guanjun Lu, Jianfei Chen, Jiong Liang, Jiahui Yang, Maoxia Li, Xinmei Zhou, Qianjin Yang, Guanjun Lu, Jianfei Chen, Jiong |
| collection | PubMed - marine biology |
| contents | A vaccine combining ORF132 and ORF25 expressed by Saccharomyces cerevisiae induces protective immunity in Carassius auratus gibelio against CyHV-2. Liang, Jiahui Yang, Maoxia Li, Xinmei Zhou, Qianjin Yang, Guanjun Lu, Jianfei Chen, Jiong Animals Fish Diseases Saccharomyces cerevisiae Goldfish Herpesviridae Infections Herpesviridae Viral Proteins Viral Vaccines Vaccines, Combined Administration, Oral CyHV-2 is the pathogen of herpesvirus hematopoietic necrosis (HVHN), resulting in significant economic losses in crucian carp. Although multiple oral vaccines have been developed to prevent CyHV-2, they have not achieved ideal protective effects. To improve the protective effect of oral vaccine, a combination vaccine was conducted by mixing recombinant Saccharomyces cerevisiae displaying ORF132 or ORF25 on the cell surface in a 1:1 ratio. Oral immunization with this combined vaccine induced specific antibodies against ORF132 and ORF25 in fish serum, as well as increased the transcription levels of IgT, IgM, IL-1β, IFN-1, and TNF-α in the spleen, head-kidney, and hindgut. Importantly, the combined vaccine provided significant protection against CyHV-2 infection in crucian carp, resulting in a relative percent survival of 80.77 %. Additionally, it suppressed viral load and alleviated pathological damage of CyHV-2-infected fish. The results suggest that the combined vaccine exhibits a better protective effect compared to the single-antigen vaccines, indicating it as a promising approach to prevent CyHV-2 outbreaks. |
| format | Artículo científico |
| id | pubmed_39732378 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | A vaccine combining ORF132 and ORF25 expressed by Saccharomyces cerevisiae induces protective immunity in Carassius auratus gibelio against CyHV-2. Liang, Jiahui Yang, Maoxia Li, Xinmei Zhou, Qianjin Yang, Guanjun Lu, Jianfei Chen, Jiong Animals Fish Diseases Saccharomyces cerevisiae Goldfish Herpesviridae Infections Herpesviridae Viral Proteins Viral Vaccines Vaccines, Combined Administration, Oral A vaccine combining ORF132 and ORF25 expressed by Saccharomyces cerevisiae induces protective immunity in Carassius auratus gibelio against CyHV-2. Liang, Jiahui Yang, Maoxia Li, Xinmei Zhou, Qianjin Yang, Guanjun Lu, Jianfei Chen, Jiong Animals Fish Diseases Saccharomyces cerevisiae Goldfish Herpesviridae Infections Herpesviridae Viral Proteins Viral Vaccines Vaccines, Combined Administration, Oral CyHV-2 is the pathogen of herpesvirus hematopoietic necrosis (HVHN), resulting in significant economic losses in crucian carp. Although multiple oral vaccines have been developed to prevent CyHV-2, they have not achieved ideal protective effects. To improve the protective effect of oral vaccine, a combination vaccine was conducted by mixing recombinant Saccharomyces cerevisiae displaying ORF132 or ORF25 on the cell surface in a 1:1 ratio. Oral immunization with this combined vaccine induced specific antibodies against ORF132 and ORF25 in fish serum, as well as increased the transcription levels of IgT, IgM, IL-1β, IFN-1, and TNF-α in the spleen, head-kidney, and hindgut. Importantly, the combined vaccine provided significant protection against CyHV-2 infection in crucian carp, resulting in a relative percent survival of 80.77 %. Additionally, it suppressed viral load and alleviated pathological damage of CyHV-2-infected fish. The results suggest that the combined vaccine exhibits a better protective effect compared to the single-antigen vaccines, indicating it as a promising approach to prevent CyHV-2 outbreaks. |
| title | A vaccine combining ORF132 and ORF25 expressed by Saccharomyces cerevisiae induces protective immunity in Carassius auratus gibelio against CyHV-2. |
| topic | Animals Fish Diseases Saccharomyces cerevisiae Goldfish Herpesviridae Infections Herpesviridae Viral Proteins Viral Vaccines Vaccines, Combined Administration, Oral |
| url | https://pubmed.ncbi.nlm.nih.gov/39732378/ |