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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of food science
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39736136/ |
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Table of Contents:
- Extraction, characterization, and hemostatic effect of collagen from the scales of Megalonibea fusca. Li, Guangfeng Guo, Tengfei Chen, Huaizhong Huang, Zhihao Chen, Qunjie Wu, Chaoxi Wang, Yifei Animals Collagen Hemostatics Amino Acids Oligochaeta Pepsin A Electrophoresis, Polyacrylamide Gel Humans Collagen Type I Marine collagen is gaining more attraction than terrestrial collagen because it is free of zoonotic disease and religious constrain. In this study, we aimed to investigate and compare the physicochemical properties and functional characteristics of acid-soluble collagen (ASC-MF) and pepsin-soluble collagen (PSC-MF) extracted from scales of Megalonibea fusca. ASC-MF and PSC-MF were evaluated in terms of yield, collagen type, amino acid composition, thermal stability, microstructure, cytotoxicity, and other physicochemical parameters. ASC-MF and PSC-MF depicted 1.72 ± 0.2% and 11.72 ± 0.3% of dry weight yields, respectively, and were identified as type I collagen with an intact triple-helical structure by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), spectroscopic analysis, and electron microscopy. Additionally, compared with many temperate and tropical species, ASC-MF and PSC-MF showed higher thermal stability, with the maximum transition temperature (T) of 53.50°C (ASC-MF) and 43.16°C (PSC-MF). CCK-8 assay showed that ASC-MF and PSC-MF have no cytotoxicity in vitro. The determination of blood clotting index values showed that both ASC-MF and PSC-MF had good hemostatic ability. In summary, these findings show that PSC-MF isolated from the scales of M. fusca may be a feasible alternative to terrestrial collagen sources in food and biomedical applications.