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| Hauptverfasser: | , , , , , , , , , , , |
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| Format: | Artículo científico |
| Sprache: | en |
| Veröffentlicht: |
Journal of applied microbiology
2025
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| Schlagworte: | |
| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/39749841/ |
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Inhaltsangabe:
- Flavuside B exhibits antioxidant and anti-inflammatory properties in Staphylococcus aureus infected skin wound and affect the expression of genes controlling bacterial quorum sensing. Chingizova, Ekaterina A Yurchenko, Ekaterina A Starnovskaya, Sofya S Chingizov, Artur R Kuzmich, Aleksandra S Pislyagin, Evgeny A Vasilchenko, Alexey S Poshvina, Darya V Shilovsky, Gregory A Dibrova, Daria V Aminin, Dmitry L Yurchenko, Anton N Humans Staphylococcus aureus Quorum Sensing Antioxidants Keratinocytes Anti-Inflammatory Agents HaCaT Cells Skin Wound Healing Reactive Oxygen Species Cell Line The aim of this study was to evaluate the antioxidant and anti-inflammatory effects of marine fungal cerebroside flavuside B (FlaB) on Staphylococcus aureus-infected keratinocytes in in vitro skin wounds and to identify FlaB targets in bacterial and human cells. A combination of enzyme-linked immunosorbent assay (ELISA), plate spectrofluorimetry, and flow cytometry with fluorescence dye staining, scratch assay, and real-time cell imaging techniques was used to investigate the effects of FlaB on S. aureus-infected HaCaT keratinocytes. FlaB decreased reactive oxygen species levels, nitrite oxide levels, and TNF-α and IL-18 release in S. aureus-infected HaCaT cells. FlaB reversed the inhibition of HaCaT cell proliferation caused by S. aureus infection. FlaB significantly increased keratinocyte migration and wound healing in an in vitro S. aureus-infected wound skin model. Using real-time qPCR, we found that FlaB caused a 1.7-fold reduction in agrA expression, which controls quorum sensing system in S. aureus. Bioinformatics analysis and molecular docking, together with experimental data, suggest that FlaB targets the pro/antioxidant defense system in human cells. Thus, FlaB can play a dual role as an antibacterial and pro/antioxidant machinery modulator, providing an observable positive effect in S. aureus-infected in vitro skin wounds. Staphylococcal sortase A enzyme and Arg systems are the targets of FlaB in bacterial cells. Nrf2/Bach1 dependent pro/antioxidant defense system is a target of FlaB in human cells. Some suggestions have also been made regarding the biological role of this marine fungal metabolite and its therapeutic possibilities.