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Main Authors:	Zborowsky, Sophia, Tahan, Ran, Lindell, Debbie
Format:	Artículo científico
Language:	en
Published:	Nature microbiology 2025
Subjects:	Synechococcus Bacteriophages RNA, Transfer
Online Access:	https://pubmed.ncbi.nlm.nih.gov/39753669/
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author	Zborowsky, Sophia Tahan, Ran Lindell, Debbie
author_facet	Zborowsky, Sophia Tahan, Ran Lindell, Debbie Zborowsky, Sophia Tahan, Ran Lindell, Debbie
collection	PubMed - marine biology
contents	Adaptive loss of tRNA gene expression leads to phage resistance in a marine Synechococcus cyanobacterium. Zborowsky, Sophia Tahan, Ran Lindell, Debbie Synechococcus Bacteriophages RNA, Transfer Synechococcus is a significant primary producer in the oceans, coexisting with cyanophages, which are important agents of mortality. Bacterial resistance against phage infection is a topic of significant interest, yet little is known for ecologically relevant systems. Here we use exogenous gene expression and gene disruption to investigate mechanisms underlying intracellular resistance of marine Synechococcus WH5701 to the Syn9 cyanophage. The restriction-modification and Gabija defence systems possessed by Synechococcus WH5701 did not contribute to resistance. Instead, resistance was primarily driven by insufficient levels of Leu tRNA, preventing translation of key phage genes in a passive, intracellular mode of resistance. Restoring cellular tRNA expression rendered the cyanobacterium sensitive to infection. We propose an evolutionary scenario whereby changes in cell codon usage, acquisition of tRNAs by the phage and loss of cell and phage tRNA expression resulted in an effective means of resistance, highlighting the dynamic interplay between bacteria and phages in shaping their co-evolutionary trajectories.
format	Artículo científico
id	pubmed_39753669
institution	PubMed
language	en
publishDate	2025
publisher	Nature microbiology
record_format	pubmed
spellingShingle	Adaptive loss of tRNA gene expression leads to phage resistance in a marine Synechococcus cyanobacterium. Zborowsky, Sophia Tahan, Ran Lindell, Debbie Synechococcus Bacteriophages RNA, Transfer Adaptive loss of tRNA gene expression leads to phage resistance in a marine Synechococcus cyanobacterium. Zborowsky, Sophia Tahan, Ran Lindell, Debbie Synechococcus Bacteriophages RNA, Transfer Synechococcus is a significant primary producer in the oceans, coexisting with cyanophages, which are important agents of mortality. Bacterial resistance against phage infection is a topic of significant interest, yet little is known for ecologically relevant systems. Here we use exogenous gene expression and gene disruption to investigate mechanisms underlying intracellular resistance of marine Synechococcus WH5701 to the Syn9 cyanophage. The restriction-modification and Gabija defence systems possessed by Synechococcus WH5701 did not contribute to resistance. Instead, resistance was primarily driven by insufficient levels of Leu tRNA, preventing translation of key phage genes in a passive, intracellular mode of resistance. Restoring cellular tRNA expression rendered the cyanobacterium sensitive to infection. We propose an evolutionary scenario whereby changes in cell codon usage, acquisition of tRNAs by the phage and loss of cell and phage tRNA expression resulted in an effective means of resistance, highlighting the dynamic interplay between bacteria and phages in shaping their co-evolutionary trajectories.
title	Adaptive loss of tRNA gene expression leads to phage resistance in a marine Synechococcus cyanobacterium.
topic	Synechococcus Bacteriophages RNA, Transfer
url	https://pubmed.ncbi.nlm.nih.gov/39753669/

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