Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Zoological research
2025
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39757016/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266260615135234 |
|---|---|
| author | Nie, Li Wu, Xiang-Yu Zhao, Zi-Yue Fei, Chen-Jie Zhu, Ting-Fang Shao, Jian-Zhong Chen, Jiong |
| author_facet | Nie, Li Wu, Xiang-Yu Zhao, Zi-Yue Fei, Chen-Jie Zhu, Ting-Fang Shao, Jian-Zhong Chen, Jiong Nie, Li Wu, Xiang-Yu Zhao, Zi-Yue Fei, Chen-Jie Zhu, Ting-Fang Shao, Jian-Zhong Chen, Jiong |
| collection | PubMed - marine biology |
| contents | Palmitoylation-mediated NLRP3 inflammasome activation in teleosts highlights evolutionary divergence in immune regulation. Nie, Li Wu, Xiang-Yu Zhao, Zi-Yue Fei, Chen-Jie Zhu, Ting-Fang Shao, Jian-Zhong Chen, Jiong Animals NLR Family, Pyrin Domain-Containing 3 Protein Inflammasomes Lipoylation Zebrafish Biological Evolution Immunity, Innate Perciformes NLRP3 inflammasome activation is pivotal for cytokine secretion and pyroptosis in response to diverse stimuli, playing a crucial role in innate immunity. While extensively studied in mammals, the regulatory mechanisms governing NLRP3 activation in non-mammalian vertebrates remain largely unexplored. Teleosts, as basal vertebrates, represent an ideal model for exploring the evolutionary trajectory of inflammasome regulation. In this study, ABE assays, confocal microscopy, and biochemical analyses were applied to systematically characterize the mechanisms underlying NLRP3 inflammasome in teleosts, using large yellow croakers ( , ) and zebrafish ( , ) as representative models. Our findings revealed a previously unrecognized palmitoylation-dependent regulatory mechanism essential for teleost NLRP3 activation. Specifically, zDHHC18-mediated palmitoylation at a teleost-specific cysteine residue (C946 in NLRP3, C1037 in NLRP3) was required for the translocation of NLRP3 to the dispersed trans-Golgi network, facilitating its subsequent recruitment to the microtubule-organizing center. This membrane trafficking was crucial for inflammasome assembly and downstream inflammatory responses. These findings provide new insights into the distinct regulatory mechanisms of NLRP3 activation in teleosts, highlighting an evolutionary divergence that contributes to innate immunity adaptation in early vertebrates. |
| format | Artículo científico |
| id | pubmed_39757016 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Zoological research |
| record_format | pubmed |
| spellingShingle | Palmitoylation-mediated NLRP3 inflammasome activation in teleosts highlights evolutionary divergence in immune regulation. Nie, Li Wu, Xiang-Yu Zhao, Zi-Yue Fei, Chen-Jie Zhu, Ting-Fang Shao, Jian-Zhong Chen, Jiong Animals NLR Family, Pyrin Domain-Containing 3 Protein Inflammasomes Lipoylation Zebrafish Biological Evolution Immunity, Innate Perciformes Palmitoylation-mediated NLRP3 inflammasome activation in teleosts highlights evolutionary divergence in immune regulation. Nie, Li Wu, Xiang-Yu Zhao, Zi-Yue Fei, Chen-Jie Zhu, Ting-Fang Shao, Jian-Zhong Chen, Jiong Animals NLR Family, Pyrin Domain-Containing 3 Protein Inflammasomes Lipoylation Zebrafish Biological Evolution Immunity, Innate Perciformes NLRP3 inflammasome activation is pivotal for cytokine secretion and pyroptosis in response to diverse stimuli, playing a crucial role in innate immunity. While extensively studied in mammals, the regulatory mechanisms governing NLRP3 activation in non-mammalian vertebrates remain largely unexplored. Teleosts, as basal vertebrates, represent an ideal model for exploring the evolutionary trajectory of inflammasome regulation. In this study, ABE assays, confocal microscopy, and biochemical analyses were applied to systematically characterize the mechanisms underlying NLRP3 inflammasome in teleosts, using large yellow croakers ( , ) and zebrafish ( , ) as representative models. Our findings revealed a previously unrecognized palmitoylation-dependent regulatory mechanism essential for teleost NLRP3 activation. Specifically, zDHHC18-mediated palmitoylation at a teleost-specific cysteine residue (C946 in NLRP3, C1037 in NLRP3) was required for the translocation of NLRP3 to the dispersed trans-Golgi network, facilitating its subsequent recruitment to the microtubule-organizing center. This membrane trafficking was crucial for inflammasome assembly and downstream inflammatory responses. These findings provide new insights into the distinct regulatory mechanisms of NLRP3 activation in teleosts, highlighting an evolutionary divergence that contributes to innate immunity adaptation in early vertebrates. |
| title | Palmitoylation-mediated NLRP3 inflammasome activation in teleosts highlights evolutionary divergence in immune regulation. |
| topic | Animals NLR Family, Pyrin Domain-Containing 3 Protein Inflammasomes Lipoylation Zebrafish Biological Evolution Immunity, Innate Perciformes |
| url | https://pubmed.ncbi.nlm.nih.gov/39757016/ |