Nucleosome Spacing Can Fine-Tune Higher Order Chromatin Assembly.
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| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
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bioRxiv : the preprint server for biology
2024
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| _version_ | 1868266260607795200 |
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| author | Chen, Lifeng Maristany, M Julia Farr, Stephen E Luo, Jinyue Gibson, Bryan A Doolittle, Lynda K Espinosa, Jorge R Huertas, Jan Redding, Sy Collepardo-Guevara, Rosana Rosen, Michael K |
| author_facet | Chen, Lifeng Maristany, M Julia Farr, Stephen E Luo, Jinyue Gibson, Bryan A Doolittle, Lynda K Espinosa, Jorge R Huertas, Jan Redding, Sy Collepardo-Guevara, Rosana Rosen, Michael K Chen, Lifeng Maristany, M Julia Farr, Stephen E Luo, Jinyue Gibson, Bryan A Doolittle, Lynda K Espinosa, Jorge R Huertas, Jan Redding, Sy Collepardo-Guevara, Rosana Rosen, Michael K |
| collection | PubMed - marine biology |
| contents | Nucleosome Spacing Can Fine-Tune Higher Order Chromatin Assembly. Chen, Lifeng Maristany, M Julia Farr, Stephen E Luo, Jinyue Gibson, Bryan A Doolittle, Lynda K Espinosa, Jorge R Huertas, Jan Redding, Sy Collepardo-Guevara, Rosana Rosen, Michael K Cellular chromatin displays heterogeneous structure and dynamics, properties that control diverse nuclear processes. Models invoke phase separation of conformational ensembles of chromatin fibers as a mechanism regulating chromatin organization . Here we combine biochemistry and molecular dynamics simulations to examine, at single base-pair resolution, how nucleosome spacing controls chromatin phase separation. We show that as DNA linkers extend from 25 bp to 30 bp, as examplars of 10N+5 and 10N (integer N) bp lengths, chromatin condensates become less thermodynamically stable and nucleosome mobility increases. Simulations reveal that this is due to trade-offs between inter- and intramolecular nucleosome stacking, favored by rigid 10N+5 and 10N bp linkers, respectively. A remodeler can induce or inhibit phase separation by moving nucleosomes, changing the balance between intra- and intermolecular stacking. The intrinsic phase separation capacity of chromatin enables fine tuning of compaction and dynamics, likely contributing to heterogeneous chromatin organization . |
| format | Artículo científico |
| id | pubmed_39763792 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | bioRxiv : the preprint server for biology |
| record_format | pubmed |
| spellingShingle | Nucleosome Spacing Can Fine-Tune Higher Order Chromatin Assembly. Chen, Lifeng Maristany, M Julia Farr, Stephen E Luo, Jinyue Gibson, Bryan A Doolittle, Lynda K Espinosa, Jorge R Huertas, Jan Redding, Sy Collepardo-Guevara, Rosana Rosen, Michael K Nucleosome Spacing Can Fine-Tune Higher Order Chromatin Assembly. Chen, Lifeng Maristany, M Julia Farr, Stephen E Luo, Jinyue Gibson, Bryan A Doolittle, Lynda K Espinosa, Jorge R Huertas, Jan Redding, Sy Collepardo-Guevara, Rosana Rosen, Michael K Cellular chromatin displays heterogeneous structure and dynamics, properties that control diverse nuclear processes. Models invoke phase separation of conformational ensembles of chromatin fibers as a mechanism regulating chromatin organization . Here we combine biochemistry and molecular dynamics simulations to examine, at single base-pair resolution, how nucleosome spacing controls chromatin phase separation. We show that as DNA linkers extend from 25 bp to 30 bp, as examplars of 10N+5 and 10N (integer N) bp lengths, chromatin condensates become less thermodynamically stable and nucleosome mobility increases. Simulations reveal that this is due to trade-offs between inter- and intramolecular nucleosome stacking, favored by rigid 10N+5 and 10N bp linkers, respectively. A remodeler can induce or inhibit phase separation by moving nucleosomes, changing the balance between intra- and intermolecular stacking. The intrinsic phase separation capacity of chromatin enables fine tuning of compaction and dynamics, likely contributing to heterogeneous chromatin organization . |
| title | Nucleosome Spacing Can Fine-Tune Higher Order Chromatin Assembly. |
| url | https://pubmed.ncbi.nlm.nih.gov/39763792/ |