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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
International journal of molecular sciences
2024
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/39769456/ |
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| _version_ | 1868266258162515968 |
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| author | Kim, Eun-A Kang, Nalae Heo, Jun-Ho Park, Areumi Heo, Seong-Yeong Kim, Hyun-Soo Heo, Soo-Jin |
| author_facet | Kim, Eun-A Kang, Nalae Heo, Jun-Ho Park, Areumi Heo, Seong-Yeong Kim, Hyun-Soo Heo, Soo-Jin Kim, Eun-A Kang, Nalae Heo, Jun-Ho Park, Areumi Heo, Seong-Yeong Kim, Hyun-Soo Heo, Soo-Jin |
| collection | PubMed - marine biology |
| contents | Antiviral Activity of Extracts and Dieckol Against Zika Virus. Kim, Eun-A Kang, Nalae Heo, Jun-Ho Park, Areumi Heo, Seong-Yeong Kim, Hyun-Soo Heo, Soo-Jin Zika Virus Antiviral Agents Animals Chlorocebus aethiops Vero Cells Benzofurans Phaeophyceae Molecular Docking Simulation Virus Replication Plant Extracts Zika Virus Infection Viral Nonstructural Proteins RNA-Dependent RNA Polymerase Humans Viral Proteases Serine Endopeptidases Nucleoside-Triphosphatase DEAD-box RNA Helicases and its major compound dieckol, both natural marine products, possess antioxidant, anti-inflammatory, and metabolic-regulating effects. Zika virus (ZIKV), an arbovirus from the family, is transmitted by mosquitoes and causes serious illnesses in humans. This study aimed to evaluate the anti-ZIKV potential of and dieckol. The antiviral activity of extract (ECE), prepared with 80% ethanol, was assessed in ZIKV-infected Vero E6 cells through MTT assay, plaque assay, and quantitative polymerase chain reaction (qPCR), demonstrating no cytotoxicity and a significant reduction in viral titers and ZIKV mRNA levels. In addition, ECE decreased the expression of tumor necrosis factor-α and interferon-induced protein with tetratricopeptide repeats in the ZIKV-infected cells. Dieckol, the primary active compound in ECE, exhibited potent anti-ZIKV activity, with a half maximal inhibitory concentration (IC), value of 4.8 µM. In silico molecular docking analysis revealed that dieckol forms stable complexes with key ZIKV proteins, including the envelope, NS2B/NS3, and RNA-dependent RNA polymerase (RdRp) protein, exhibiting high binding energies of -438.09 kcal/mol, -1040.51 kcal/mol, and -1043.40 kcal/mol, respectively. Overall, our findings suggest that ECE and dieckol are promising candidates for the development of anti-ZIKV agents. |
| format | Artículo científico |
| id | pubmed_39769456 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | International journal of molecular sciences |
| record_format | pubmed |
| spellingShingle | Antiviral Activity of Extracts and Dieckol Against Zika Virus. Kim, Eun-A Kang, Nalae Heo, Jun-Ho Park, Areumi Heo, Seong-Yeong Kim, Hyun-Soo Heo, Soo-Jin Zika Virus Antiviral Agents Animals Chlorocebus aethiops Vero Cells Benzofurans Phaeophyceae Molecular Docking Simulation Virus Replication Plant Extracts Zika Virus Infection Viral Nonstructural Proteins RNA-Dependent RNA Polymerase Humans Viral Proteases Serine Endopeptidases Nucleoside-Triphosphatase DEAD-box RNA Helicases Antiviral Activity of Extracts and Dieckol Against Zika Virus. Kim, Eun-A Kang, Nalae Heo, Jun-Ho Park, Areumi Heo, Seong-Yeong Kim, Hyun-Soo Heo, Soo-Jin Zika Virus Antiviral Agents Animals Chlorocebus aethiops Vero Cells Benzofurans Phaeophyceae Molecular Docking Simulation Virus Replication Plant Extracts Zika Virus Infection Viral Nonstructural Proteins RNA-Dependent RNA Polymerase Humans Viral Proteases Serine Endopeptidases Nucleoside-Triphosphatase DEAD-box RNA Helicases and its major compound dieckol, both natural marine products, possess antioxidant, anti-inflammatory, and metabolic-regulating effects. Zika virus (ZIKV), an arbovirus from the family, is transmitted by mosquitoes and causes serious illnesses in humans. This study aimed to evaluate the anti-ZIKV potential of and dieckol. The antiviral activity of extract (ECE), prepared with 80% ethanol, was assessed in ZIKV-infected Vero E6 cells through MTT assay, plaque assay, and quantitative polymerase chain reaction (qPCR), demonstrating no cytotoxicity and a significant reduction in viral titers and ZIKV mRNA levels. In addition, ECE decreased the expression of tumor necrosis factor-α and interferon-induced protein with tetratricopeptide repeats in the ZIKV-infected cells. Dieckol, the primary active compound in ECE, exhibited potent anti-ZIKV activity, with a half maximal inhibitory concentration (IC), value of 4.8 µM. In silico molecular docking analysis revealed that dieckol forms stable complexes with key ZIKV proteins, including the envelope, NS2B/NS3, and RNA-dependent RNA polymerase (RdRp) protein, exhibiting high binding energies of -438.09 kcal/mol, -1040.51 kcal/mol, and -1043.40 kcal/mol, respectively. Overall, our findings suggest that ECE and dieckol are promising candidates for the development of anti-ZIKV agents. |
| title | Antiviral Activity of Extracts and Dieckol Against Zika Virus. |
| topic | Zika Virus Antiviral Agents Animals Chlorocebus aethiops Vero Cells Benzofurans Phaeophyceae Molecular Docking Simulation Virus Replication Plant Extracts Zika Virus Infection Viral Nonstructural Proteins RNA-Dependent RNA Polymerase Humans Viral Proteases Serine Endopeptidases Nucleoside-Triphosphatase DEAD-box RNA Helicases |
| url | https://pubmed.ncbi.nlm.nih.gov/39769456/ |