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Main Authors:	Gu, Jiayu, Zhang, Xinqiao, Liu, Tianlang, Guo, Yunxue
Format:	Artículo científico
Language:	en
Published:	Viruses 2024
Subjects:	Pseudomonas aeruginosa Pseudomonas Phages Biofilms Wastewater Phage Therapy Genome, Viral Gene Expression Profiling
Online Access:	https://pubmed.ncbi.nlm.nih.gov/39772127/
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author	Gu, Jiayu Zhang, Xinqiao Liu, Tianlang Guo, Yunxue
author_facet	Gu, Jiayu Zhang, Xinqiao Liu, Tianlang Guo, Yunxue Gu, Jiayu Zhang, Xinqiao Liu, Tianlang Guo, Yunxue
collection	PubMed - marine biology
contents	Isolation and Characterization of a Lytic Phage PaTJ Against . Gu, Jiayu Zhang, Xinqiao Liu, Tianlang Guo, Yunxue Pseudomonas aeruginosa Pseudomonas Phages Biofilms Wastewater Phage Therapy Genome, Viral Gene Expression Profiling is a major global threat to human health, and phage therapy has emerged as a promising strategy for treating infections caused by multidrug-resistant pathogens. In this study, we isolated and characterized a lytic phage, PaTJ, from wastewater. PaTJ belongs to the phage family , and is featured by short latency (30 min) and large burst size (10 PFU per infected cell). Our investigation revealed that PaTJ utilizes the type IV Pili (T4P) as a receptor. Transcriptome analysis of PaTJ infected host at latent stage showed distinct expression patterns of PaTJ encoding genes involved in replication and structure assembly, without expression of the majority of toxic accessory genes responsible for phage release. In addition, host bacteria exhibited specific induction of host metabolism-related genes in response to the PaTJ's infection. Furthermore, our findings demonstrated the PaTJ's potential in degrading biofilms. This work sheds light on the multifaceted impact of this lytic phage PaTJ on , presenting potential applications in both gene expression modulation and biofilm management.
format	Artículo científico
id	pubmed_39772127
institution	PubMed
language	en
publishDate	2024
publisher	Viruses
record_format	pubmed
spellingShingle	Isolation and Characterization of a Lytic Phage PaTJ Against . Gu, Jiayu Zhang, Xinqiao Liu, Tianlang Guo, Yunxue Pseudomonas aeruginosa Pseudomonas Phages Biofilms Wastewater Phage Therapy Genome, Viral Gene Expression Profiling Isolation and Characterization of a Lytic Phage PaTJ Against . Gu, Jiayu Zhang, Xinqiao Liu, Tianlang Guo, Yunxue Pseudomonas aeruginosa Pseudomonas Phages Biofilms Wastewater Phage Therapy Genome, Viral Gene Expression Profiling is a major global threat to human health, and phage therapy has emerged as a promising strategy for treating infections caused by multidrug-resistant pathogens. In this study, we isolated and characterized a lytic phage, PaTJ, from wastewater. PaTJ belongs to the phage family , and is featured by short latency (30 min) and large burst size (10 PFU per infected cell). Our investigation revealed that PaTJ utilizes the type IV Pili (T4P) as a receptor. Transcriptome analysis of PaTJ infected host at latent stage showed distinct expression patterns of PaTJ encoding genes involved in replication and structure assembly, without expression of the majority of toxic accessory genes responsible for phage release. In addition, host bacteria exhibited specific induction of host metabolism-related genes in response to the PaTJ's infection. Furthermore, our findings demonstrated the PaTJ's potential in degrading biofilms. This work sheds light on the multifaceted impact of this lytic phage PaTJ on , presenting potential applications in both gene expression modulation and biofilm management.
title	Isolation and Characterization of a Lytic Phage PaTJ Against .
topic	Pseudomonas aeruginosa Pseudomonas Phages Biofilms Wastewater Phage Therapy Genome, Viral Gene Expression Profiling
url	https://pubmed.ncbi.nlm.nih.gov/39772127/

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