Peripheral B Lymphocyte Serves as a Reservoir for the Persistently Covert Infection of Mandarin Fish Ranavirus.
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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Viruses
2024
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| _version_ | 1868266258157273089 |
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| author | Zhang, Wenfeng Gong, Hui Sun, Qianqian Fu, Yuting Wu, Xiaosi Deng, Hengwei Weng, Shaoping He, Jianguo Dong, Chuanfu |
| author_facet | Zhang, Wenfeng Gong, Hui Sun, Qianqian Fu, Yuting Wu, Xiaosi Deng, Hengwei Weng, Shaoping He, Jianguo Dong, Chuanfu Zhang, Wenfeng Gong, Hui Sun, Qianqian Fu, Yuting Wu, Xiaosi Deng, Hengwei Weng, Shaoping He, Jianguo Dong, Chuanfu |
| collection | PubMed - marine biology |
| contents | Peripheral B Lymphocyte Serves as a Reservoir for the Persistently Covert Infection of Mandarin Fish Ranavirus. Zhang, Wenfeng Gong, Hui Sun, Qianqian Fu, Yuting Wu, Xiaosi Deng, Hengwei Weng, Shaoping He, Jianguo Dong, Chuanfu Animals Ranavirus DNA Virus Infections B-Lymphocytes Fish Diseases Viral Load Persistent Infection Disease Reservoirs Fishes Mandarin fish ranavirus (MRV) is a distinctive member among the genus of the family . The persistently covert infection of MRV was previously observed in a natural outbreak of MRV, but the underlying mechanism remains unclear. Here, we show that mandarin fish peripheral B lymphocytes are implemented as viral reservoirs to maintain the persistent infection. When mandarin fish were infected with a sublethal dosage of MRV under a nonpermissive temperature (19 °C) and a permissive temperature (26 °C), all of the fish in the 19 °C group survived and entered the persistent phase of infection, characterized by a very low viral load in white blood cells, whereas some of the fish died of MRV infection in the 26 °C group, and the survival fish then initiated a persistent infection status. Raising the temperature, vaccination and dexamethasone treatment can reactivate the quiescent MRV to replicate and result in partial mortality. The viral reservoir investigation showed that IgM-labeled B lymphocytes, but not CD3Δ-labeled T lymphocytes and MRC-1-labeled macrophages, are target cells for the persistent infection of MRV. Moreover, the reactivation of the quiescent MRV was confirmed through a non-TLR5 signal pathway manner. Collectively, we demonstrate the presence of the B cell-dependent persistent infection of ranavirus, and provide a new clue for better understanding the complex infection mechanism of vertebrate iridovirus. |
| format | Artículo científico |
| id | pubmed_39772201 |
| institution | PubMed |
| language | en |
| publishDate | 2024 |
| publisher | Viruses |
| record_format | pubmed |
| spellingShingle | Peripheral B Lymphocyte Serves as a Reservoir for the Persistently Covert Infection of Mandarin Fish Ranavirus. Zhang, Wenfeng Gong, Hui Sun, Qianqian Fu, Yuting Wu, Xiaosi Deng, Hengwei Weng, Shaoping He, Jianguo Dong, Chuanfu Animals Ranavirus DNA Virus Infections B-Lymphocytes Fish Diseases Viral Load Persistent Infection Disease Reservoirs Fishes Peripheral B Lymphocyte Serves as a Reservoir for the Persistently Covert Infection of Mandarin Fish Ranavirus. Zhang, Wenfeng Gong, Hui Sun, Qianqian Fu, Yuting Wu, Xiaosi Deng, Hengwei Weng, Shaoping He, Jianguo Dong, Chuanfu Animals Ranavirus DNA Virus Infections B-Lymphocytes Fish Diseases Viral Load Persistent Infection Disease Reservoirs Fishes Mandarin fish ranavirus (MRV) is a distinctive member among the genus of the family . The persistently covert infection of MRV was previously observed in a natural outbreak of MRV, but the underlying mechanism remains unclear. Here, we show that mandarin fish peripheral B lymphocytes are implemented as viral reservoirs to maintain the persistent infection. When mandarin fish were infected with a sublethal dosage of MRV under a nonpermissive temperature (19 °C) and a permissive temperature (26 °C), all of the fish in the 19 °C group survived and entered the persistent phase of infection, characterized by a very low viral load in white blood cells, whereas some of the fish died of MRV infection in the 26 °C group, and the survival fish then initiated a persistent infection status. Raising the temperature, vaccination and dexamethasone treatment can reactivate the quiescent MRV to replicate and result in partial mortality. The viral reservoir investigation showed that IgM-labeled B lymphocytes, but not CD3Δ-labeled T lymphocytes and MRC-1-labeled macrophages, are target cells for the persistent infection of MRV. Moreover, the reactivation of the quiescent MRV was confirmed through a non-TLR5 signal pathway manner. Collectively, we demonstrate the presence of the B cell-dependent persistent infection of ranavirus, and provide a new clue for better understanding the complex infection mechanism of vertebrate iridovirus. |
| title | Peripheral B Lymphocyte Serves as a Reservoir for the Persistently Covert Infection of Mandarin Fish Ranavirus. |
| topic | Animals Ranavirus DNA Virus Infections B-Lymphocytes Fish Diseases Viral Load Persistent Infection Disease Reservoirs Fishes |
| url | https://pubmed.ncbi.nlm.nih.gov/39772201/ |