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Main Authors:	Yang, Yu-Chen S H, Tsai, Chung-Che, Yang, Yung-Ning, Liu, Feng-Cheng, Lee, Sheng-Yang, Yang, Jen-Chang, Crawford, Dana R, Chiu, Hsien-Chung, Lu, Mei-Chin, Li, Zi-Lin, Chen, Yi-Chen, Chu, Tin-Yi, Whang-Peng, Jacqueline, Lin, Hung-Yun, Wang, Kuan
Format:	Artículo científico
Language:	en
Published:	Oncology reports 2025
Subjects:	Humans Cholangiocarcinoma Epidermal Growth Factor Cell Proliferation Signal Transduction B7-H1 Antigen Cell Line, Tumor Phosphatidylinositol 3-Kinases Bile Duct Neoplasms Gene Expression Regulation, Neoplastic Cell Survival
Online Access:	https://pubmed.ncbi.nlm.nih.gov/39791224/
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author	Yang, Yu-Chen S H Tsai, Chung-Che Yang, Yung-Ning Liu, Feng-Cheng Lee, Sheng-Yang Yang, Jen-Chang Crawford, Dana R Chiu, Hsien-Chung Lu, Mei-Chin Li, Zi-Lin Chen, Yi-Chen Chu, Tin-Yi Whang-Peng, Jacqueline Lin, Hung-Yun Wang, Kuan
author_facet	Yang, Yu-Chen S H Tsai, Chung-Che Yang, Yung-Ning Liu, Feng-Cheng Lee, Sheng-Yang Yang, Jen-Chang Crawford, Dana R Chiu, Hsien-Chung Lu, Mei-Chin Li, Zi-Lin Chen, Yi-Chen Chu, Tin-Yi Whang-Peng, Jacqueline Lin, Hung-Yun Wang, Kuan Yang, Yu-Chen S H Tsai, Chung-Che Yang, Yung-Ning Liu, Feng-Cheng Lee, Sheng-Yang Yang, Jen-Chang Crawford, Dana R Chiu, Hsien-Chung Lu, Mei-Chin Li, Zi-Lin Chen, Yi-Chen Chu, Tin-Yi Whang-Peng, Jacqueline Lin, Hung-Yun Wang, Kuan
collection	PubMed - marine biology
contents	Heteronemin suppresses EGF‑induced proliferation through the PI3K/PD‑L1 signaling pathways in cholangiocarcinoma. Yang, Yu-Chen S H Tsai, Chung-Che Yang, Yung-Ning Liu, Feng-Cheng Lee, Sheng-Yang Yang, Jen-Chang Crawford, Dana R Chiu, Hsien-Chung Lu, Mei-Chin Li, Zi-Lin Chen, Yi-Chen Chu, Tin-Yi Whang-Peng, Jacqueline Lin, Hung-Yun Wang, Kuan Humans Cholangiocarcinoma Epidermal Growth Factor Cell Proliferation Signal Transduction B7-H1 Antigen Cell Line, Tumor Phosphatidylinositol 3-Kinases Bile Duct Neoplasms Gene Expression Regulation, Neoplastic Cell Survival Epidermal growth factor (EGF) binds with its surface receptor to stimulate gene expression and cancer cell proliferation. EGF stimulates cancer cell growth via phosphoinositide 3‑kinase (PI3K) and programmed cell death ligand 1 (PD‑L1) pathways. As an integrin αvβ3 antagonist, heteronemin exhibits potent cytotoxic effects against cancer cells. It inhibits critical signal transduction pathways promoted by the EGF. In the current study, EGF‑induced signal activation and proliferative effects were investigated in cholangiocarcinoma cells and its molecular targets using qPCR and western blotting analyses. In addition, cell viability assays were performed to assess the growth effects of EGF and heteronemin. Heteronemin reversed the effects of EGF and was further enhanced by blockage of PI3K's activity. In summary, EGF stimulates cholangiocarcinoma cell growth. On the other hand, heteronemin inhibited PI3K activation and PD‑L1 expression to reverse the stimulative effects of EGF‑induced gene expression and proliferation in cholangiocarcinoma cells.
format	Artículo científico
id	pubmed_39791224
institution	PubMed
language	en
publishDate	2025
publisher	Oncology reports
record_format	pubmed
spellingShingle	Heteronemin suppresses EGF‑induced proliferation through the PI3K/PD‑L1 signaling pathways in cholangiocarcinoma. Yang, Yu-Chen S H Tsai, Chung-Che Yang, Yung-Ning Liu, Feng-Cheng Lee, Sheng-Yang Yang, Jen-Chang Crawford, Dana R Chiu, Hsien-Chung Lu, Mei-Chin Li, Zi-Lin Chen, Yi-Chen Chu, Tin-Yi Whang-Peng, Jacqueline Lin, Hung-Yun Wang, Kuan Humans Cholangiocarcinoma Epidermal Growth Factor Cell Proliferation Signal Transduction B7-H1 Antigen Cell Line, Tumor Phosphatidylinositol 3-Kinases Bile Duct Neoplasms Gene Expression Regulation, Neoplastic Cell Survival Heteronemin suppresses EGF‑induced proliferation through the PI3K/PD‑L1 signaling pathways in cholangiocarcinoma. Yang, Yu-Chen S H Tsai, Chung-Che Yang, Yung-Ning Liu, Feng-Cheng Lee, Sheng-Yang Yang, Jen-Chang Crawford, Dana R Chiu, Hsien-Chung Lu, Mei-Chin Li, Zi-Lin Chen, Yi-Chen Chu, Tin-Yi Whang-Peng, Jacqueline Lin, Hung-Yun Wang, Kuan Humans Cholangiocarcinoma Epidermal Growth Factor Cell Proliferation Signal Transduction B7-H1 Antigen Cell Line, Tumor Phosphatidylinositol 3-Kinases Bile Duct Neoplasms Gene Expression Regulation, Neoplastic Cell Survival Epidermal growth factor (EGF) binds with its surface receptor to stimulate gene expression and cancer cell proliferation. EGF stimulates cancer cell growth via phosphoinositide 3‑kinase (PI3K) and programmed cell death ligand 1 (PD‑L1) pathways. As an integrin αvβ3 antagonist, heteronemin exhibits potent cytotoxic effects against cancer cells. It inhibits critical signal transduction pathways promoted by the EGF. In the current study, EGF‑induced signal activation and proliferative effects were investigated in cholangiocarcinoma cells and its molecular targets using qPCR and western blotting analyses. In addition, cell viability assays were performed to assess the growth effects of EGF and heteronemin. Heteronemin reversed the effects of EGF and was further enhanced by blockage of PI3K's activity. In summary, EGF stimulates cholangiocarcinoma cell growth. On the other hand, heteronemin inhibited PI3K activation and PD‑L1 expression to reverse the stimulative effects of EGF‑induced gene expression and proliferation in cholangiocarcinoma cells.
title	Heteronemin suppresses EGF‑induced proliferation through the PI3K/PD‑L1 signaling pathways in cholangiocarcinoma.
topic	Humans Cholangiocarcinoma Epidermal Growth Factor Cell Proliferation Signal Transduction B7-H1 Antigen Cell Line, Tumor Phosphatidylinositol 3-Kinases Bile Duct Neoplasms Gene Expression Regulation, Neoplastic Cell Survival
url	https://pubmed.ncbi.nlm.nih.gov/39791224/

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