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| Auteurs principaux: | , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
International journal of biological macromolecules
2025
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| Sujets: | |
| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/39793815/ |
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| _version_ | 1868266258133155840 |
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| author | Lin, Tianxiang Liu, Lu Zeng, Liang Zhao, Congxin Xiao, Shu Ma, Haitao Li, Jun Mao, Fan Qin, Yanping Zhang, Yuehuan Zhang, Yang Xiang, Zhiming Yu, Ziniu |
| author_facet | Lin, Tianxiang Liu, Lu Zeng, Liang Zhao, Congxin Xiao, Shu Ma, Haitao Li, Jun Mao, Fan Qin, Yanping Zhang, Yuehuan Zhang, Yang Xiang, Zhiming Yu, Ziniu Lin, Tianxiang Liu, Lu Zeng, Liang Zhao, Congxin Xiao, Shu Ma, Haitao Li, Jun Mao, Fan Qin, Yanping Zhang, Yuehuan Zhang, Yang Xiang, Zhiming Yu, Ziniu |
| collection | PubMed - marine biology |
| contents | ChNLRC4, a cytoplasmic pattern recognition receptor, activates the pyroptosis signaling pathway in Mollusca. Lin, Tianxiang Liu, Lu Zeng, Liang Zhao, Congxin Xiao, Shu Ma, Haitao Li, Jun Mao, Fan Qin, Yanping Zhang, Yuehuan Zhang, Yang Xiang, Zhiming Yu, Ziniu Animals Pyroptosis Signal Transduction Humans HEK293 Cells Crassostrea Receptors, Pattern Recognition Caspase 1 Hemocytes Mollusca Cytoplasm Inflammasomes NLR inflammasomes recognize pathogen-associated molecular patterns (PAMPs), triggering Caspase-1 activation and leading to gasdermin D (GSDMD)-mediated pyroptosis, a crucial immune response in mammals. The functional GSDME-mediated pyroptosis has been reported in invertebrates, yet the existence of an NLR-Caspase-GSDME axis mediating pyroptosis signaling cascades remains unclear. In this study, we reported an NLRC4 homolog named ChNLRC4, a pattern recognition receptor from the oyster Crassostrea hongkongensis that is able to bind to LPS and Lys-type PGN through its LRR domain. ChNLRC4 interacted with ChCaspase-1 through CARD-CARD domain homotypic interactions and enhanced ChCaspase-1 activity. Additionally, overexpression of ChNLRC4 promoted ChCaspase-1-mediated cleavage of ChGSDME, leading to pyroptosis in HEK293T cells. Furthermore, knockdown of chnlrc4 resulted in a significant reduction in the death rate of hemocytes, immune infiltration of hemocytes, cilium shedding, and bacterial clearance. Collectively, this study provides insight into the role of NLR within the pyroptosis signaling pathway in oysters. |
| format | Artículo científico |
| id | pubmed_39793815 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of biological macromolecules |
| record_format | pubmed |
| spellingShingle | ChNLRC4, a cytoplasmic pattern recognition receptor, activates the pyroptosis signaling pathway in Mollusca. Lin, Tianxiang Liu, Lu Zeng, Liang Zhao, Congxin Xiao, Shu Ma, Haitao Li, Jun Mao, Fan Qin, Yanping Zhang, Yuehuan Zhang, Yang Xiang, Zhiming Yu, Ziniu Animals Pyroptosis Signal Transduction Humans HEK293 Cells Crassostrea Receptors, Pattern Recognition Caspase 1 Hemocytes Mollusca Cytoplasm Inflammasomes ChNLRC4, a cytoplasmic pattern recognition receptor, activates the pyroptosis signaling pathway in Mollusca. Lin, Tianxiang Liu, Lu Zeng, Liang Zhao, Congxin Xiao, Shu Ma, Haitao Li, Jun Mao, Fan Qin, Yanping Zhang, Yuehuan Zhang, Yang Xiang, Zhiming Yu, Ziniu Animals Pyroptosis Signal Transduction Humans HEK293 Cells Crassostrea Receptors, Pattern Recognition Caspase 1 Hemocytes Mollusca Cytoplasm Inflammasomes NLR inflammasomes recognize pathogen-associated molecular patterns (PAMPs), triggering Caspase-1 activation and leading to gasdermin D (GSDMD)-mediated pyroptosis, a crucial immune response in mammals. The functional GSDME-mediated pyroptosis has been reported in invertebrates, yet the existence of an NLR-Caspase-GSDME axis mediating pyroptosis signaling cascades remains unclear. In this study, we reported an NLRC4 homolog named ChNLRC4, a pattern recognition receptor from the oyster Crassostrea hongkongensis that is able to bind to LPS and Lys-type PGN through its LRR domain. ChNLRC4 interacted with ChCaspase-1 through CARD-CARD domain homotypic interactions and enhanced ChCaspase-1 activity. Additionally, overexpression of ChNLRC4 promoted ChCaspase-1-mediated cleavage of ChGSDME, leading to pyroptosis in HEK293T cells. Furthermore, knockdown of chnlrc4 resulted in a significant reduction in the death rate of hemocytes, immune infiltration of hemocytes, cilium shedding, and bacterial clearance. Collectively, this study provides insight into the role of NLR within the pyroptosis signaling pathway in oysters. |
| title | ChNLRC4, a cytoplasmic pattern recognition receptor, activates the pyroptosis signaling pathway in Mollusca. |
| topic | Animals Pyroptosis Signal Transduction Humans HEK293 Cells Crassostrea Receptors, Pattern Recognition Caspase 1 Hemocytes Mollusca Cytoplasm Inflammasomes |
| url | https://pubmed.ncbi.nlm.nih.gov/39793815/ |