Generation of glucosylantimycins by heterologous expression of a promiscuous glycosyltransferase in a deepsea-derived .
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| Autores principales: | , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Natural product research
2026
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| _version_ | 1868266255338700801 |
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| author | Duan, He Zhang, Meifang Wang, Xin Xiao, Fei Li, Wenli |
| author_facet | Duan, He Zhang, Meifang Wang, Xin Xiao, Fei Li, Wenli Duan, He Zhang, Meifang Wang, Xin Xiao, Fei Li, Wenli |
| collection | PubMed - marine biology |
| contents | Generation of glucosylantimycins by heterologous expression of a promiscuous glycosyltransferase in a deepsea-derived . Duan, He Zhang, Meifang Wang, Xin Xiao, Fei Li, Wenli Streptomyces Humans Glycosyltransferases HeLa Cells Molecular Structure Hep G2 Cells Glycosylation Antimycins are a class of depsipeptide compounds that exhibit diverse bioactivities. However, their potential clinical applications are hampered by high cell toxicities. Glycosylation usually has profound impacts on the physicochemical properties, bioactivities and toxicities of natural products. In this study, we overexpressed an artificial glycosyltransferase (GT) gene in deepsea-derived ZH66, leading to the discovery of three new antimycin derivatives glucosylantimycins A, B and C (-). Their structures were determined by a combination of spectroscopic methods, including high resolution electrospray ionisation mass spectrometry (HRESIMS) and nuclear magnetic resonance (NMR) analysis. The glycosylation remarkably improved the water solubility but simultaneously reduced the cytotoxicities of antimycins towards human cervix epidermoid carcinoma (HeLa) and human hepatic carcinoma (HepG2) cell lines. |
| format | Artículo científico |
| id | pubmed_39804083 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Natural product research |
| record_format | pubmed |
| spellingShingle | Generation of glucosylantimycins by heterologous expression of a promiscuous glycosyltransferase in a deepsea-derived . Duan, He Zhang, Meifang Wang, Xin Xiao, Fei Li, Wenli Streptomyces Humans Glycosyltransferases HeLa Cells Molecular Structure Hep G2 Cells Glycosylation Generation of glucosylantimycins by heterologous expression of a promiscuous glycosyltransferase in a deepsea-derived . Duan, He Zhang, Meifang Wang, Xin Xiao, Fei Li, Wenli Streptomyces Humans Glycosyltransferases HeLa Cells Molecular Structure Hep G2 Cells Glycosylation Antimycins are a class of depsipeptide compounds that exhibit diverse bioactivities. However, their potential clinical applications are hampered by high cell toxicities. Glycosylation usually has profound impacts on the physicochemical properties, bioactivities and toxicities of natural products. In this study, we overexpressed an artificial glycosyltransferase (GT) gene in deepsea-derived ZH66, leading to the discovery of three new antimycin derivatives glucosylantimycins A, B and C (-). Their structures were determined by a combination of spectroscopic methods, including high resolution electrospray ionisation mass spectrometry (HRESIMS) and nuclear magnetic resonance (NMR) analysis. The glycosylation remarkably improved the water solubility but simultaneously reduced the cytotoxicities of antimycins towards human cervix epidermoid carcinoma (HeLa) and human hepatic carcinoma (HepG2) cell lines. |
| title | Generation of glucosylantimycins by heterologous expression of a promiscuous glycosyltransferase in a deepsea-derived . |
| topic | Streptomyces Humans Glycosyltransferases HeLa Cells Molecular Structure Hep G2 Cells Glycosylation |
| url | https://pubmed.ncbi.nlm.nih.gov/39804083/ |