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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Biology letters
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39809328/ |
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| _version_ | 1868266255331360770 |
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| author | Brémaud, Julie Debelli, Alizée Khorami, Hajar Hosseini Stewart, Donald T Angers, Annie Angers, Bernard Breton, Sophie |
| author_facet | Brémaud, Julie Debelli, Alizée Khorami, Hajar Hosseini Stewart, Donald T Angers, Annie Angers, Bernard Breton, Sophie Brémaud, Julie Debelli, Alizée Khorami, Hajar Hosseini Stewart, Donald T Angers, Annie Angers, Bernard Breton, Sophie |
| collection | PubMed - marine biology |
| contents | Demystifying the functions of the mitochondrial ORFan proteins in bivalves with doubly uniparental inheritance. Brémaud, Julie Debelli, Alizée Khorami, Hajar Hosseini Stewart, Donald T Angers, Annie Angers, Bernard Breton, Sophie Animals Male Female Mitochondrial Proteins Open Reading Frames Mytilus edulis Bivalvia Mitochondria Strict maternal inheritance of mitochondria is known to be the rule in animals, but over 100 species across six orders of bivalves possess doubly uniparental inheritance (DUI) of mitochondria. Under DUI, two distinctive sex-specific mitogenomes coexist. In marine and freshwater mussels, each mitogenome has an additional protein-coding gene, called female- and male-specific open reading frame or and , respectively. The function(s) of the associated FORF and MORF proteins remain unknown. Herein, we show that these proteins present similar tissue expression patterns in two distantly related DUI species: MORF was only expressed in male gonads, whereas FORF was expressed in all tissues of both sexes in the marine mussel and the freshwater mussel . Moreover, MORF was only expressed during the reproductive season, while FORF presented no clear seasonality pattern in . Immunocytochemistry revealed the presence of both proteins in mitochondria and acrosomes of late spermatids and mature sperm. We hypothesize that MORF has a key function in spermatogenesis, while FORF has a more general function in both sexes. We also propose that both proteins may be involved in the fertilization process. The involvement of MORF in paternal mitochondrial transmission is also discussed. |
| format | Artículo científico |
| id | pubmed_39809328 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Biology letters |
| record_format | pubmed |
| spellingShingle | Demystifying the functions of the mitochondrial ORFan proteins in bivalves with doubly uniparental inheritance. Brémaud, Julie Debelli, Alizée Khorami, Hajar Hosseini Stewart, Donald T Angers, Annie Angers, Bernard Breton, Sophie Animals Male Female Mitochondrial Proteins Open Reading Frames Mytilus edulis Bivalvia Mitochondria Demystifying the functions of the mitochondrial ORFan proteins in bivalves with doubly uniparental inheritance. Brémaud, Julie Debelli, Alizée Khorami, Hajar Hosseini Stewart, Donald T Angers, Annie Angers, Bernard Breton, Sophie Animals Male Female Mitochondrial Proteins Open Reading Frames Mytilus edulis Bivalvia Mitochondria Strict maternal inheritance of mitochondria is known to be the rule in animals, but over 100 species across six orders of bivalves possess doubly uniparental inheritance (DUI) of mitochondria. Under DUI, two distinctive sex-specific mitogenomes coexist. In marine and freshwater mussels, each mitogenome has an additional protein-coding gene, called female- and male-specific open reading frame or and , respectively. The function(s) of the associated FORF and MORF proteins remain unknown. Herein, we show that these proteins present similar tissue expression patterns in two distantly related DUI species: MORF was only expressed in male gonads, whereas FORF was expressed in all tissues of both sexes in the marine mussel and the freshwater mussel . Moreover, MORF was only expressed during the reproductive season, while FORF presented no clear seasonality pattern in . Immunocytochemistry revealed the presence of both proteins in mitochondria and acrosomes of late spermatids and mature sperm. We hypothesize that MORF has a key function in spermatogenesis, while FORF has a more general function in both sexes. We also propose that both proteins may be involved in the fertilization process. The involvement of MORF in paternal mitochondrial transmission is also discussed. |
| title | Demystifying the functions of the mitochondrial ORFan proteins in bivalves with doubly uniparental inheritance. |
| topic | Animals Male Female Mitochondrial Proteins Open Reading Frames Mytilus edulis Bivalvia Mitochondria |
| url | https://pubmed.ncbi.nlm.nih.gov/39809328/ |