Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of xenobiotics
2025
|
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39846542/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- Nanoplastic-Induced Developmental Toxicity in Ascidians: Comparative Analysis of Chorionated and Dechorionated Embryos. Eliso, Maria Concetta Corsi, Ilaria Spagnuolo, Antonietta Dumollard, Rémi Nanoplastics pose a growing threat to marine ecosystems, particularly affecting the early developmental stages of marine organisms. This study investigates the effects of amino-modified polystyrene nanoparticles (PS-NH, 50 nm) on the embryonic development of , a model ascidian species. Both chorionated and dechorionated embryos were exposed to increasing concentrations of PS-NH so morphological alterations could be assessed with a high-content analysis of the phenotypes and genotoxicity. PS-NH induced the same morphological alterations in both chorionated and dechorionated embryos, with dechorionated embryos being more sensitive (EC = 3.0 μg mL) than chorionated ones (EC = 6.26 μg mL). Interestingly, results from the morphological analysis showed two concentration-dependent mechanisms of action: (i) at concentrations near the EC, neurodevelopmental abnormalities resembling the ones induced by exposure to known endocrine disruptors (EDs) were observed, and (ii) at higher concentrations (15 μg mL and 7.5 μg mL for chorionated and dechorionated embryos, respectively), a nonspecific toxicity was evident, likely due to general oxidative stress. The phenotypes resulting from the PS-NH treatment were not related to DNA damage, as revealed by a genotoxicity assay performed on neurula embryos. Our data suggest that PS-NH-induced toxicity is primarily mediated through oxidative stress, probably triggered by interactions between the positive charges of the PS NPs and the negative charges on the cell membranes. The lack of a protective chorion further exacerbated these effects, highlighting its role in mitigating/protecting against NP-induced damage.