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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Fish & shellfish immunology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39848419/ |
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| _version_ | 1868266252627083264 |
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| author | Sohn, Min-Young Jeong, Ji-Min Kang, Gyoungsik Woo, Won-Sik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il |
| author_facet | Sohn, Min-Young Jeong, Ji-Min Kang, Gyoungsik Woo, Won-Sik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il Sohn, Min-Young Jeong, Ji-Min Kang, Gyoungsik Woo, Won-Sik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il |
| collection | PubMed - marine biology |
| contents | Identification and characterization of CD83 and CD276 as markers of dendritic cells in olive flounder (Paralichthys olivaceus). Sohn, Min-Young Jeong, Ji-Min Kang, Gyoungsik Woo, Won-Sik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il Animals Antigens, CD CD83 Antigen Immunoglobulins Dendritic Cells Biomarkers Novirhabdovirus Fish Proteins Membrane Glycoproteins Viral Vaccines Flatfishes Fish Diseases Hemorrhagic Septicemia, Viral Dendritic cells (DCs) play a pivotal role in activating naïve T-cells and bridging innate and adaptive immunity. This study aimed to identify and characterize CD83 and CD276 as potential markers for DCs in olive flounder (Paralichthys olivaceus). Specific antibodies against these markers were developed and used to analyze their distribution in peripheral blood leukocytes (PBLs) and intestinal tissues. Flow cytometry and immunohistochemistry revealed that CD83 and CD276 are expressed on DCs, with peak expression observed one week after oral administration of an inactivated viral hemorrhagic septicemia virus (VHSV) vaccine. Gene expression analysis further demonstrated significant activation of immune-related genes, including CD3, IgM, and TLRs, indicating that oral vaccine administration effectively activates the intestinal mucosal immune system. These findings provide valuable insights into fish immune responses and establish CD83 and CD276 as promising DC markers, contributing to the development of mucosal vaccine strategies in aquaculture. |
| format | Artículo científico |
| id | pubmed_39848419 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | Identification and characterization of CD83 and CD276 as markers of dendritic cells in olive flounder (Paralichthys olivaceus). Sohn, Min-Young Jeong, Ji-Min Kang, Gyoungsik Woo, Won-Sik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il Animals Antigens, CD CD83 Antigen Immunoglobulins Dendritic Cells Biomarkers Novirhabdovirus Fish Proteins Membrane Glycoproteins Viral Vaccines Flatfishes Fish Diseases Hemorrhagic Septicemia, Viral Identification and characterization of CD83 and CD276 as markers of dendritic cells in olive flounder (Paralichthys olivaceus). Sohn, Min-Young Jeong, Ji-Min Kang, Gyoungsik Woo, Won-Sik Kim, Kyung-Ho Son, Ha-Jeong Park, Chan-Il Animals Antigens, CD CD83 Antigen Immunoglobulins Dendritic Cells Biomarkers Novirhabdovirus Fish Proteins Membrane Glycoproteins Viral Vaccines Flatfishes Fish Diseases Hemorrhagic Septicemia, Viral Dendritic cells (DCs) play a pivotal role in activating naïve T-cells and bridging innate and adaptive immunity. This study aimed to identify and characterize CD83 and CD276 as potential markers for DCs in olive flounder (Paralichthys olivaceus). Specific antibodies against these markers were developed and used to analyze their distribution in peripheral blood leukocytes (PBLs) and intestinal tissues. Flow cytometry and immunohistochemistry revealed that CD83 and CD276 are expressed on DCs, with peak expression observed one week after oral administration of an inactivated viral hemorrhagic septicemia virus (VHSV) vaccine. Gene expression analysis further demonstrated significant activation of immune-related genes, including CD3, IgM, and TLRs, indicating that oral vaccine administration effectively activates the intestinal mucosal immune system. These findings provide valuable insights into fish immune responses and establish CD83 and CD276 as promising DC markers, contributing to the development of mucosal vaccine strategies in aquaculture. |
| title | Identification and characterization of CD83 and CD276 as markers of dendritic cells in olive flounder (Paralichthys olivaceus). |
| topic | Animals Antigens, CD CD83 Antigen Immunoglobulins Dendritic Cells Biomarkers Novirhabdovirus Fish Proteins Membrane Glycoproteins Viral Vaccines Flatfishes Fish Diseases Hemorrhagic Septicemia, Viral |
| url | https://pubmed.ncbi.nlm.nih.gov/39848419/ |