Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Molecules (Basel, Switzerland)
2025
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39860094/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266249932242947 |
|---|---|
| author | Banti, Matteo Garcia-Gil, Mercedes Guidotti, Lorenzo Di Giuseppe, Graziano Rapposelli, Simona Monti, Daniela Tampucci, Silvia De Leo, Marinella Gado, Francesca Nieri, Paola Manera, Clementina |
| author_facet | Banti, Matteo Garcia-Gil, Mercedes Guidotti, Lorenzo Di Giuseppe, Graziano Rapposelli, Simona Monti, Daniela Tampucci, Silvia De Leo, Marinella Gado, Francesca Nieri, Paola Manera, Clementina Banti, Matteo Garcia-Gil, Mercedes Guidotti, Lorenzo Di Giuseppe, Graziano Rapposelli, Simona Monti, Daniela Tampucci, Silvia De Leo, Marinella Gado, Francesca Nieri, Paola Manera, Clementina |
| collection | PubMed - marine biology |
| contents | Characterization and Otoprotective Effects of Polysaccharides from . Banti, Matteo Garcia-Gil, Mercedes Guidotti, Lorenzo Di Giuseppe, Graziano Rapposelli, Simona Monti, Daniela Tampucci, Silvia De Leo, Marinella Gado, Francesca Nieri, Paola Manera, Clementina Spirulina Reactive Oxygen Species Animals Cisplatin Polysaccharides Oxidative Stress Cell Survival Mice Cell Line Antioxidants Protective Agents Humans Polysaccharides, Bacterial Antineoplastic Agents Organ of Corti Hearing loss is one of the most common sensory disorders in humans, and a large number of cases are due to ear cell damage caused by ototoxic drugs including anticancer agents, such as cisplatin. The recent literature reported that hearing loss is promoted by an excessive generation of reactive oxygen species (ROS) in cochlea cells, which causes oxidative stress. Recently, polysaccharides from the cyanobacterium showed many biological activities, including antioxidant activity, suggesting their potential use to combat hearing loss. On these bases, this study describes the extraction, purification, and characterization of water-soluble polysaccharides from (SPPs) and the investigation of their protective role against cisplatin toxicity on House Ear Institute-Organ of Corti (HEI-OC1) cells. The results showed that SPPs (5-80 µg/mL) induced a dose-dependent increase in viability, statistically significant at 40 µg/mL and 80 µg/mL. Moreover, SPPs, evaluated at 80 µg/mL, inhibited the cisplatin-induced ROS level increase in HEI-OC1. This evidence highlights the potential of SPPs as natural candidates to protect cochlear ear cells against ototoxic oxidative agents. Moreover, in view of the potential use of microalgal polysaccharides to realize hydrogels, SPPs could also represent a healthy carrier for other topically administered otoprotective agents. |
| format | Artículo científico |
| id | pubmed_39860094 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Molecules (Basel, Switzerland) |
| record_format | pubmed |
| spellingShingle | Characterization and Otoprotective Effects of Polysaccharides from . Banti, Matteo Garcia-Gil, Mercedes Guidotti, Lorenzo Di Giuseppe, Graziano Rapposelli, Simona Monti, Daniela Tampucci, Silvia De Leo, Marinella Gado, Francesca Nieri, Paola Manera, Clementina Spirulina Reactive Oxygen Species Animals Cisplatin Polysaccharides Oxidative Stress Cell Survival Mice Cell Line Antioxidants Protective Agents Humans Polysaccharides, Bacterial Antineoplastic Agents Organ of Corti Characterization and Otoprotective Effects of Polysaccharides from . Banti, Matteo Garcia-Gil, Mercedes Guidotti, Lorenzo Di Giuseppe, Graziano Rapposelli, Simona Monti, Daniela Tampucci, Silvia De Leo, Marinella Gado, Francesca Nieri, Paola Manera, Clementina Spirulina Reactive Oxygen Species Animals Cisplatin Polysaccharides Oxidative Stress Cell Survival Mice Cell Line Antioxidants Protective Agents Humans Polysaccharides, Bacterial Antineoplastic Agents Organ of Corti Hearing loss is one of the most common sensory disorders in humans, and a large number of cases are due to ear cell damage caused by ototoxic drugs including anticancer agents, such as cisplatin. The recent literature reported that hearing loss is promoted by an excessive generation of reactive oxygen species (ROS) in cochlea cells, which causes oxidative stress. Recently, polysaccharides from the cyanobacterium showed many biological activities, including antioxidant activity, suggesting their potential use to combat hearing loss. On these bases, this study describes the extraction, purification, and characterization of water-soluble polysaccharides from (SPPs) and the investigation of their protective role against cisplatin toxicity on House Ear Institute-Organ of Corti (HEI-OC1) cells. The results showed that SPPs (5-80 µg/mL) induced a dose-dependent increase in viability, statistically significant at 40 µg/mL and 80 µg/mL. Moreover, SPPs, evaluated at 80 µg/mL, inhibited the cisplatin-induced ROS level increase in HEI-OC1. This evidence highlights the potential of SPPs as natural candidates to protect cochlear ear cells against ototoxic oxidative agents. Moreover, in view of the potential use of microalgal polysaccharides to realize hydrogels, SPPs could also represent a healthy carrier for other topically administered otoprotective agents. |
| title | Characterization and Otoprotective Effects of Polysaccharides from . |
| topic | Spirulina Reactive Oxygen Species Animals Cisplatin Polysaccharides Oxidative Stress Cell Survival Mice Cell Line Antioxidants Protective Agents Humans Polysaccharides, Bacterial Antineoplastic Agents Organ of Corti |
| url | https://pubmed.ncbi.nlm.nih.gov/39860094/ |