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| Format: | Artículo científico |
| Language: | en |
| Published: |
The Science of the total environment
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39864248/ |
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| _version_ | 1868266249928048641 |
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| author | Baensch, Christin Kröger, Andrea Wilkes, Heinz |
| author_facet | Baensch, Christin Kröger, Andrea Wilkes, Heinz Baensch, Christin Kröger, Andrea Wilkes, Heinz |
| collection | PubMed - marine biology |
| contents | Hydrocarbon biodegradation processes at a historic oil production site - A signature metabolite study. Baensch, Christin Kröger, Andrea Wilkes, Heinz Biodegradation, Environmental Petroleum Hydrocarbons Water Pollutants, Chemical Environmental Monitoring Petroleum Pollution Soil Pollutants Gas Chromatography-Mass Spectrometry Decades of research demonstrated that microbes can remediate petroleum-contaminated environments through biodegradation of hydrocarbons. Recent studies have applied signature metabolite analysis to investigate hydrocarbon-contaminated sites, focusing primarily on aquifer systems and metabolites of relatively water-soluble monoaromatic hydrocarbons. However, the number of studies involving non-targeted analysis and identification of individual metabolites in environmental samples is limited. In addition, metabolite patterns in crude oil-contaminated samples are rarely compared to those in the unaltered crude oils. In this study, we identified metabolites in complex environmental samples and crude oils from a former oil sands mining site using non-targeted gas chromatography-mass spectrometry. Based on these results, we suggest potential biodegradation processes that may account for the formation of the observed metabolites. The non-target results from water samples contaminated with crude oil showed that aromatic acids were the dominant metabolites. We observed that arylacetic acids were particularly abundant in the water samples when the metabolite patterns were compared with those found in crude oil and oil sands samples formerly produced at the site, as well as those found in oil-contaminated soil samples. The dominance of arylacetic acids is evidence that the microbial communities have a limited capacity for α-oxidation, i.e. an enzymatic attack on the methylene group, which is necessary to further degrade these compounds. Therefore, aromatic acids may be enriched at sites with long-term exposure to crude oil. Our study demonstrates that non-targeted metabolite analysis can provide an insight into the prevalent pattern of carboxylic acid metabolites and degradation processes in hydrocarbon-contaminated habitats. |
| format | Artículo científico |
| id | pubmed_39864248 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | The Science of the total environment |
| record_format | pubmed |
| spellingShingle | Hydrocarbon biodegradation processes at a historic oil production site - A signature metabolite study. Baensch, Christin Kröger, Andrea Wilkes, Heinz Biodegradation, Environmental Petroleum Hydrocarbons Water Pollutants, Chemical Environmental Monitoring Petroleum Pollution Soil Pollutants Gas Chromatography-Mass Spectrometry Hydrocarbon biodegradation processes at a historic oil production site - A signature metabolite study. Baensch, Christin Kröger, Andrea Wilkes, Heinz Biodegradation, Environmental Petroleum Hydrocarbons Water Pollutants, Chemical Environmental Monitoring Petroleum Pollution Soil Pollutants Gas Chromatography-Mass Spectrometry Decades of research demonstrated that microbes can remediate petroleum-contaminated environments through biodegradation of hydrocarbons. Recent studies have applied signature metabolite analysis to investigate hydrocarbon-contaminated sites, focusing primarily on aquifer systems and metabolites of relatively water-soluble monoaromatic hydrocarbons. However, the number of studies involving non-targeted analysis and identification of individual metabolites in environmental samples is limited. In addition, metabolite patterns in crude oil-contaminated samples are rarely compared to those in the unaltered crude oils. In this study, we identified metabolites in complex environmental samples and crude oils from a former oil sands mining site using non-targeted gas chromatography-mass spectrometry. Based on these results, we suggest potential biodegradation processes that may account for the formation of the observed metabolites. The non-target results from water samples contaminated with crude oil showed that aromatic acids were the dominant metabolites. We observed that arylacetic acids were particularly abundant in the water samples when the metabolite patterns were compared with those found in crude oil and oil sands samples formerly produced at the site, as well as those found in oil-contaminated soil samples. The dominance of arylacetic acids is evidence that the microbial communities have a limited capacity for α-oxidation, i.e. an enzymatic attack on the methylene group, which is necessary to further degrade these compounds. Therefore, aromatic acids may be enriched at sites with long-term exposure to crude oil. Our study demonstrates that non-targeted metabolite analysis can provide an insight into the prevalent pattern of carboxylic acid metabolites and degradation processes in hydrocarbon-contaminated habitats. |
| title | Hydrocarbon biodegradation processes at a historic oil production site - A signature metabolite study. |
| topic | Biodegradation, Environmental Petroleum Hydrocarbons Water Pollutants, Chemical Environmental Monitoring Petroleum Pollution Soil Pollutants Gas Chromatography-Mass Spectrometry |
| url | https://pubmed.ncbi.nlm.nih.gov/39864248/ |