3D scaffold of hydroxyapatite/β tricalcium phosphate from mussel shells: Synthesis, characterization and cytotoxicity.
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| Format: | Artículo científico |
| Sprache: | en |
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Heliyon
2025
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| author | Fonseca, Sabrina Cunha da Freitas, Rosangela Borges Sotiles, Anne Raquel Schemczssen-Graeff, Zelinda Miranda, Inaiê Maiala De Almeida Biscaia, Stellee Marcela Petris Wypych, Fernando Silva Trindade, Edvaldo da Leão, Moira Pedroso Zielak, João César Franco, Célia Regina Cavichiolo |
| author_facet | Fonseca, Sabrina Cunha da Freitas, Rosangela Borges Sotiles, Anne Raquel Schemczssen-Graeff, Zelinda Miranda, Inaiê Maiala De Almeida Biscaia, Stellee Marcela Petris Wypych, Fernando Silva Trindade, Edvaldo da Leão, Moira Pedroso Zielak, João César Franco, Célia Regina Cavichiolo Fonseca, Sabrina Cunha da Freitas, Rosangela Borges Sotiles, Anne Raquel Schemczssen-Graeff, Zelinda Miranda, Inaiê Maiala De Almeida Biscaia, Stellee Marcela Petris Wypych, Fernando Silva Trindade, Edvaldo da Leão, Moira Pedroso Zielak, João César Franco, Célia Regina Cavichiolo |
| collection | PubMed - marine biology |
| contents | 3D scaffold of hydroxyapatite/β tricalcium phosphate from mussel shells: Synthesis, characterization and cytotoxicity. Fonseca, Sabrina Cunha da Freitas, Rosangela Borges Sotiles, Anne Raquel Schemczssen-Graeff, Zelinda Miranda, Inaiê Maiala De Almeida Biscaia, Stellee Marcela Petris Wypych, Fernando Silva Trindade, Edvaldo da Leão, Moira Pedroso Zielak, João César Franco, Célia Regina Cavichiolo Bone tissue substitutes are increasing in importance. Hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) act as a cell matrix and improve its mechanical properties. One of their raw materials is marine-origin by-products. To synthesize, characterize, and evaluate the cellular cytotoxicity of a 3D biomaterial based on HA and β-TCP from mussel shells. We prepared pellets with 150, 200, and 250 mg and evaluated them through sintering, XRD, FTIR, ICP-OES, Scanning Electron Microscopic (SEM), and immunocytochemical tests. The Alamar Blue method was applied to the Balb-T3T cell line within 72 h to evaluate cytotoxicity. Our biomaterials presented a smooth surface with slight irregularity and porosities presenting different diameters and morphologies and showed chemical, morphological, and ultrastructural similarity to bone hydroxyapatite, mainly the 150 and 200 mg pellets. We produced promising HA/β-TCP bioceramics with characteristics that allowed cell culture, promoting adhesion, spreading, and proliferation. |
| format | Artículo científico |
| id | pubmed_39866499 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Heliyon |
| record_format | pubmed |
| spellingShingle | 3D scaffold of hydroxyapatite/β tricalcium phosphate from mussel shells: Synthesis, characterization and cytotoxicity. Fonseca, Sabrina Cunha da Freitas, Rosangela Borges Sotiles, Anne Raquel Schemczssen-Graeff, Zelinda Miranda, Inaiê Maiala De Almeida Biscaia, Stellee Marcela Petris Wypych, Fernando Silva Trindade, Edvaldo da Leão, Moira Pedroso Zielak, João César Franco, Célia Regina Cavichiolo 3D scaffold of hydroxyapatite/β tricalcium phosphate from mussel shells: Synthesis, characterization and cytotoxicity. Fonseca, Sabrina Cunha da Freitas, Rosangela Borges Sotiles, Anne Raquel Schemczssen-Graeff, Zelinda Miranda, Inaiê Maiala De Almeida Biscaia, Stellee Marcela Petris Wypych, Fernando Silva Trindade, Edvaldo da Leão, Moira Pedroso Zielak, João César Franco, Célia Regina Cavichiolo Bone tissue substitutes are increasing in importance. Hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) act as a cell matrix and improve its mechanical properties. One of their raw materials is marine-origin by-products. To synthesize, characterize, and evaluate the cellular cytotoxicity of a 3D biomaterial based on HA and β-TCP from mussel shells. We prepared pellets with 150, 200, and 250 mg and evaluated them through sintering, XRD, FTIR, ICP-OES, Scanning Electron Microscopic (SEM), and immunocytochemical tests. The Alamar Blue method was applied to the Balb-T3T cell line within 72 h to evaluate cytotoxicity. Our biomaterials presented a smooth surface with slight irregularity and porosities presenting different diameters and morphologies and showed chemical, morphological, and ultrastructural similarity to bone hydroxyapatite, mainly the 150 and 200 mg pellets. We produced promising HA/β-TCP bioceramics with characteristics that allowed cell culture, promoting adhesion, spreading, and proliferation. |
| title | 3D scaffold of hydroxyapatite/β tricalcium phosphate from mussel shells: Synthesis, characterization and cytotoxicity. |
| url | https://pubmed.ncbi.nlm.nih.gov/39866499/ |