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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
BMC biology
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/39875888/ |
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| _version_ | 1868266249908125696 |
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| author | Liu, Yi Bian, Chao Ma, Ka Yan Yang, Yexin Wang, Yuanyuan Liu, Chao Ouyang, Guochang Xu, Meng Sun, Jinhui Shao, Changwei Chen, Jiehu Shi, Qiong Mu, Xidong |
| author_facet | Liu, Yi Bian, Chao Ma, Ka Yan Yang, Yexin Wang, Yuanyuan Liu, Chao Ouyang, Guochang Xu, Meng Sun, Jinhui Shao, Changwei Chen, Jiehu Shi, Qiong Mu, Xidong Liu, Yi Bian, Chao Ma, Ka Yan Yang, Yexin Wang, Yuanyuan Liu, Chao Ouyang, Guochang Xu, Meng Sun, Jinhui Shao, Changwei Chen, Jiehu Shi, Qiong Mu, Xidong |
| collection | PubMed - marine biology |
| contents | Reference genome provide insights into sex determination of silver aworana (Osteoglossum bicirrhosum). Liu, Yi Bian, Chao Ma, Ka Yan Yang, Yexin Wang, Yuanyuan Liu, Chao Ouyang, Guochang Xu, Meng Sun, Jinhui Shao, Changwei Chen, Jiehu Shi, Qiong Mu, Xidong Animals Male Female Sex Determination Processes Genome Fishes Silver arowana (Osteoglossum bicirrhosum) is a basal fish species with sexual monomorphism, while its sex determination mechanism has been poorly understood, posing a significant challenge to its captive breeding efforts. We constructed two high-quality chromosome-level genome assemblies for both female and male silver arowana, with scaffold N50 values over 10 Mb. Combining re-sequencing data of 109 individuals, we identified a female-specific region, which was localized in a non-coding region, i.e., around 26-kb upstream of foxl2 gene (encoding forkhead box L2). Its strong interaction with the neighboring foxl2 on the same chromosome suggests foxl2 as a candidate sex-related gene in silver arowana. We subsequently propose a complex gene network in the sex determination process of silver arowana, with foxl2 acting as the central contributor. Transcriptome sequencing of gonads support our hypothesis that the regulation of foxl2 can be influenced by the spatial proximity of the female-specific fragment, thereby promoting ovarian function or inhibiting testicular function to stimulate gonadal differentiation. Furthermore, we found the sex chromosomes to be homomorphic with a potentially recent origin, as a linkage disequilibrium analysis proved minor recombination suppression. These results taken together serve as a crucial foundation for conducting extensive investigations on the evolution and differentiation of sex-determining mechanisms, as well as the emergence and development of sex chromosomes in various fishes. |
| format | Artículo científico |
| id | pubmed_39875888 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | BMC biology |
| record_format | pubmed |
| spellingShingle | Reference genome provide insights into sex determination of silver aworana (Osteoglossum bicirrhosum). Liu, Yi Bian, Chao Ma, Ka Yan Yang, Yexin Wang, Yuanyuan Liu, Chao Ouyang, Guochang Xu, Meng Sun, Jinhui Shao, Changwei Chen, Jiehu Shi, Qiong Mu, Xidong Animals Male Female Sex Determination Processes Genome Fishes Reference genome provide insights into sex determination of silver aworana (Osteoglossum bicirrhosum). Liu, Yi Bian, Chao Ma, Ka Yan Yang, Yexin Wang, Yuanyuan Liu, Chao Ouyang, Guochang Xu, Meng Sun, Jinhui Shao, Changwei Chen, Jiehu Shi, Qiong Mu, Xidong Animals Male Female Sex Determination Processes Genome Fishes Silver arowana (Osteoglossum bicirrhosum) is a basal fish species with sexual monomorphism, while its sex determination mechanism has been poorly understood, posing a significant challenge to its captive breeding efforts. We constructed two high-quality chromosome-level genome assemblies for both female and male silver arowana, with scaffold N50 values over 10 Mb. Combining re-sequencing data of 109 individuals, we identified a female-specific region, which was localized in a non-coding region, i.e., around 26-kb upstream of foxl2 gene (encoding forkhead box L2). Its strong interaction with the neighboring foxl2 on the same chromosome suggests foxl2 as a candidate sex-related gene in silver arowana. We subsequently propose a complex gene network in the sex determination process of silver arowana, with foxl2 acting as the central contributor. Transcriptome sequencing of gonads support our hypothesis that the regulation of foxl2 can be influenced by the spatial proximity of the female-specific fragment, thereby promoting ovarian function or inhibiting testicular function to stimulate gonadal differentiation. Furthermore, we found the sex chromosomes to be homomorphic with a potentially recent origin, as a linkage disequilibrium analysis proved minor recombination suppression. These results taken together serve as a crucial foundation for conducting extensive investigations on the evolution and differentiation of sex-determining mechanisms, as well as the emergence and development of sex chromosomes in various fishes. |
| title | Reference genome provide insights into sex determination of silver aworana (Osteoglossum bicirrhosum). |
| topic | Animals Male Female Sex Determination Processes Genome Fishes |
| url | https://pubmed.ncbi.nlm.nih.gov/39875888/ |