Specific modulation of 28S_Um2402 rRNA 2'--ribose methylation as a novel epitranscriptomic marker of ZEB1-induced epithelial-mesenchymal transition in different mammary cell contexts.
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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NAR cancer
2025
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| _version_ | 1868266249904979970 |
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| author | Morin, Chloé Paraqindes, Hermes Van Long, Flora Nguyen Isaac, Caroline Thomas, Emilie Pedri, Dennis Pulido-Vicuna, Carlos Ariel Morel, Anne-Pierre Marchand, Virginie Motorin, Yuri Carrere, Marjorie Auclair, Jessie Attignon, Valéry Pommier, Roxane M Ruiz, Emmanuelle Bourdelais, Fleur Catez, Frédéric Durand, Sébastien Ferrari, Anthony Viari, Alain Marine, Jean-Christophe Puisieux, Alain Diaz, Jean-Jacques Moyret-Lalle, Caroline Marcel, Virginie |
| author_facet | Morin, Chloé Paraqindes, Hermes Van Long, Flora Nguyen Isaac, Caroline Thomas, Emilie Pedri, Dennis Pulido-Vicuna, Carlos Ariel Morel, Anne-Pierre Marchand, Virginie Motorin, Yuri Carrere, Marjorie Auclair, Jessie Attignon, Valéry Pommier, Roxane M Ruiz, Emmanuelle Bourdelais, Fleur Catez, Frédéric Durand, Sébastien Ferrari, Anthony Viari, Alain Marine, Jean-Christophe Puisieux, Alain Diaz, Jean-Jacques Moyret-Lalle, Caroline Marcel, Virginie Morin, Chloé Paraqindes, Hermes Van Long, Flora Nguyen Isaac, Caroline Thomas, Emilie Pedri, Dennis Pulido-Vicuna, Carlos Ariel Morel, Anne-Pierre Marchand, Virginie Motorin, Yuri Carrere, Marjorie Auclair, Jessie Attignon, Valéry Pommier, Roxane M Ruiz, Emmanuelle Bourdelais, Fleur Catez, Frédéric Durand, Sébastien Ferrari, Anthony Viari, Alain Marine, Jean-Christophe Puisieux, Alain Diaz, Jean-Jacques Moyret-Lalle, Caroline Marcel, Virginie |
| collection | PubMed - marine biology |
| contents | Specific modulation of 28S_Um2402 rRNA 2'--ribose methylation as a novel epitranscriptomic marker of ZEB1-induced epithelial-mesenchymal transition in different mammary cell contexts. Morin, Chloé Paraqindes, Hermes Van Long, Flora Nguyen Isaac, Caroline Thomas, Emilie Pedri, Dennis Pulido-Vicuna, Carlos Ariel Morel, Anne-Pierre Marchand, Virginie Motorin, Yuri Carrere, Marjorie Auclair, Jessie Attignon, Valéry Pommier, Roxane M Ruiz, Emmanuelle Bourdelais, Fleur Catez, Frédéric Durand, Sébastien Ferrari, Anthony Viari, Alain Marine, Jean-Christophe Puisieux, Alain Diaz, Jean-Jacques Moyret-Lalle, Caroline Marcel, Virginie Epithelial-Mesenchymal Transition Humans Zinc Finger E-box-Binding Homeobox 1 Female Methylation Zinc Finger E-box Binding Homeobox 2 RNA, Ribosomal, 28S Cell Line, Tumor RNA, Small Nucleolar Triple Negative Breast Neoplasms Twist-Related Protein 1 Gene Expression Regulation, Neoplastic Epigenesis, Genetic Nuclear Proteins The epithelial-mesenchymal transition (EMT) is a dynamic transdifferentiation of epithelial cells into mesenchymal cells. EMT programs exhibit great diversity, based primarily on the distinct impact of molecular activities of the EMT transcription factors. Using a panel of cancer cell lines and a series of 71 triple-negative primary breast tumors, we report that the EMT transcription factor ZEB1 modulates site-specific chemical modifications of ribosomal RNA (rRNA). Overexpression of ZEB1 and ZEB2, but not TWIST1, decreased the level of 2'--ribose methylation (2'Ome) of 28S rRNA at position Um2402. ZEB1 overexpression specifically reduced the expression of the corresponding C/D box small nucleolar RNAs (snoRNAs) SNORD143/144, which guide the rRNA 2'Ome complex at the 28S_Um2402 site. During ZEB1-induced EMT induction/reversion, the levels of both 2'Ome at 28S_Um2402 and SNORD143/144 were dynamically comodulated. Taken together, these data demonstrate that 2'Ome rRNA epitranscriptomics is a novel marker of ZEB1-induced EMT. |
| format | Artículo científico |
| id | pubmed_39877292 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | NAR cancer |
| record_format | pubmed |
| spellingShingle | Specific modulation of 28S_Um2402 rRNA 2'--ribose methylation as a novel epitranscriptomic marker of ZEB1-induced epithelial-mesenchymal transition in different mammary cell contexts. Morin, Chloé Paraqindes, Hermes Van Long, Flora Nguyen Isaac, Caroline Thomas, Emilie Pedri, Dennis Pulido-Vicuna, Carlos Ariel Morel, Anne-Pierre Marchand, Virginie Motorin, Yuri Carrere, Marjorie Auclair, Jessie Attignon, Valéry Pommier, Roxane M Ruiz, Emmanuelle Bourdelais, Fleur Catez, Frédéric Durand, Sébastien Ferrari, Anthony Viari, Alain Marine, Jean-Christophe Puisieux, Alain Diaz, Jean-Jacques Moyret-Lalle, Caroline Marcel, Virginie Epithelial-Mesenchymal Transition Humans Zinc Finger E-box-Binding Homeobox 1 Female Methylation Zinc Finger E-box Binding Homeobox 2 RNA, Ribosomal, 28S Cell Line, Tumor RNA, Small Nucleolar Triple Negative Breast Neoplasms Twist-Related Protein 1 Gene Expression Regulation, Neoplastic Epigenesis, Genetic Nuclear Proteins Specific modulation of 28S_Um2402 rRNA 2'--ribose methylation as a novel epitranscriptomic marker of ZEB1-induced epithelial-mesenchymal transition in different mammary cell contexts. Morin, Chloé Paraqindes, Hermes Van Long, Flora Nguyen Isaac, Caroline Thomas, Emilie Pedri, Dennis Pulido-Vicuna, Carlos Ariel Morel, Anne-Pierre Marchand, Virginie Motorin, Yuri Carrere, Marjorie Auclair, Jessie Attignon, Valéry Pommier, Roxane M Ruiz, Emmanuelle Bourdelais, Fleur Catez, Frédéric Durand, Sébastien Ferrari, Anthony Viari, Alain Marine, Jean-Christophe Puisieux, Alain Diaz, Jean-Jacques Moyret-Lalle, Caroline Marcel, Virginie Epithelial-Mesenchymal Transition Humans Zinc Finger E-box-Binding Homeobox 1 Female Methylation Zinc Finger E-box Binding Homeobox 2 RNA, Ribosomal, 28S Cell Line, Tumor RNA, Small Nucleolar Triple Negative Breast Neoplasms Twist-Related Protein 1 Gene Expression Regulation, Neoplastic Epigenesis, Genetic Nuclear Proteins The epithelial-mesenchymal transition (EMT) is a dynamic transdifferentiation of epithelial cells into mesenchymal cells. EMT programs exhibit great diversity, based primarily on the distinct impact of molecular activities of the EMT transcription factors. Using a panel of cancer cell lines and a series of 71 triple-negative primary breast tumors, we report that the EMT transcription factor ZEB1 modulates site-specific chemical modifications of ribosomal RNA (rRNA). Overexpression of ZEB1 and ZEB2, but not TWIST1, decreased the level of 2'--ribose methylation (2'Ome) of 28S rRNA at position Um2402. ZEB1 overexpression specifically reduced the expression of the corresponding C/D box small nucleolar RNAs (snoRNAs) SNORD143/144, which guide the rRNA 2'Ome complex at the 28S_Um2402 site. During ZEB1-induced EMT induction/reversion, the levels of both 2'Ome at 28S_Um2402 and SNORD143/144 were dynamically comodulated. Taken together, these data demonstrate that 2'Ome rRNA epitranscriptomics is a novel marker of ZEB1-induced EMT. |
| title | Specific modulation of 28S_Um2402 rRNA 2'--ribose methylation as a novel epitranscriptomic marker of ZEB1-induced epithelial-mesenchymal transition in different mammary cell contexts. |
| topic | Epithelial-Mesenchymal Transition Humans Zinc Finger E-box-Binding Homeobox 1 Female Methylation Zinc Finger E-box Binding Homeobox 2 RNA, Ribosomal, 28S Cell Line, Tumor RNA, Small Nucleolar Triple Negative Breast Neoplasms Twist-Related Protein 1 Gene Expression Regulation, Neoplastic Epigenesis, Genetic Nuclear Proteins |
| url | https://pubmed.ncbi.nlm.nih.gov/39877292/ |